Quantification of endogenous and exogenous protein expressions of Na,K-ATPase with super-resolution PALM/STORM imaging. [PDF]
PLoS ONE, 2018 Transient transfection of fluorescent fusion proteins is a key enabling technology in fluorescent microscopy to spatio-temporally map cellular protein distributions.
Kristoffer Bernhem +2 more
doaj +1 more source
Fluorescence In Situ Hybridization Method for Measuring Transfection Efficiency
BioTechniques, 1996 We describe here the use of fluorescence in situ hybridization (FISH) to measure the transfection efficiency of the transient expression vector pCMVcat in lymphoblasts and fibroblasts.
Lie Cheng, Corazon D. Bucana, Qingyi Wei
doaj +1 more source
Oncogenic microRNA-4534 regulates PTEN pathway in prostate cancer. [PDF]
, 2016 Prostate carcinogenesis involves alterations in several signaling pathways, the most prominent being the PI3K/AKT pathway. This pathway is constitutively active and drives prostate cancer (PCa) progression to advanced metastatic disease. PTEN, a critical
Chowdhary, Harshika +19 more
core +2 more sources
Scientific Reports, 2021 Infection of hepatocytes by hepatitis B virus (HBV) depends on surface expression of its receptor Na+-taurocholate-cotransporting polypeptide (NTCP), but sufficient NTCP expression is lacking in most cell lines.
Andreas Oswald +5 more
doaj +1 more source
Functional expression of TRPV4 channels in human collecting duct cells: implications for secondary hypertension in diabetic nephropathy [PDF]
, 2012 Background. The Vanilloid subfamily of transient receptor potential (TRPV) ion channels has been widely implicated in detecting osmotic and mechanical stress.
Bland, Rosemary +2 more
core +3 more sources
Genome editing in potato via CRISPR-Cas9 ribonucleoprotein delivery [PDF]
, 2018 Clustered regularly interspaced short palindromic repeats and CRISPR-associated protein-9 (CRISPR-Cas9) can be used as an efficient tool for genome editing in potato (Solanum tuberosum).
Andersson +22 more
core +1 more source
cAMP Signaling Enhances HIV-1 Long Terminal Repeat (LTR)-directed Transcription and Viral Replication in Bone Marrow Progenitor Cells. [PDF]
, 2017 CD34+ hematopoietic progenitor cells have been shown to be susceptible to HIV-1 infection, possibly due to a low-level expression of CXCR4, a coreceptor for HIV-1 entry.
Banerjee, Anupam +5 more
core +3 more sources
Localization of the Major NF-κB-activating Site and the Sole TRAF3 Binding Site of LMP-1 Defines Two Distinct Signaling Motifs [PDF]
, 1997 The TRAF3 molecule interacts with the cytoplasmic carboxyl terminus (COOH terminus) of the Epstein-Barr virus-encoded oncogene LMP-1. NF-κB activation is a downstream signaling event of tumor necrosis factor receptor-associated factor (TRAF) molecules in
Baltimore, David +3 more
core +1 more source
High efficiency transfection of thymic epithelial cell lines and primary thymic epithelial cells by Nucleofection [PDF]
, 2011 Thymic epithelial cells (TECs) are required for the development and differentiation of T cells and are sufficient for the positive and negative selection of developing T cells.
Brian G. Condie +2 more
core +2 more sources
Suitability of a CMV/EGFP cassette to monitor stable expression from human artificial chromosomes but not transient transfer in the cells forming viable clones [PDF]
, 2004 Human artificial chromosomes (HACs) were generated by transfer of telomerized PAC constructs containing alpha satellite DNA of various human chromosomes. To monitor which cells took up constructs and subsequently formed stable clones under blasticidin S (
Christan, S. +3 more
core +1 more source