Sodium Current Measurements in HEK293 Cells
Bio-Protocol, 2013 This protocol is used to measure sodium currents from heterologously transfected cells lines, such as HEK293 cells. Standard whole cell patch clamp technique is used to assess ion channel function. This protocol has been used to study cardiac type Nav1.5
Helena Riuró +5 more
doaj +2 more sources
Dose-Response, 2018 Metal nanoparticles are widely used in industry, agriculture, textiles, drugs, and so on. The adverse effect of green platinum nanoparticles on human embryonic kidney (HEK293) cells is not well established.
Rafa S. Almeer +4 more
doaj +2 more sources
Antioxidant Efficacy of Esculetin against Tert-Butyl Hydroperoxide-Induced Oxidative Stress in HEK293 Cells [PDF]
Current Issues in Molecular Biology, 2022 Esculetin is an antioxidant and anti-inflammatory compound derived from coumarin. Oxidative stress can cause overproduction of reactive oxygen species (ROS), which can lead to the development of chronic kidney failure.
Woo Kwon Jung +4 more
doaj +2 more sources
Endogenous calcium channels in human embryonic kidney (HEK293) cells [PDF]
British Journal of Pharmacology, 1996 We have identified endogenous calcium channel currents in HEK293 cells. Whole cell endogenous currents (ISr‐HEK) were studied in single HEK293 cells with 10 mM strontium as the charge carrier by the patch clamp technique. The kinetic properties and pharmacological features of ISr‐HEK were characterized and compared with the properties of a ...
Manfred Grabner, Hartmut H Glossmann
exaly +3 more sources
Human Mas-related G protein-coupled receptors-X1 induce chemokine receptor 2 expression in rat dorsal root ganglia neurons and release of chemokine ligand 2 from the human LAD-2 mast cell line [PDF]
, 2013 Primate-specific Mas-related G protein-coupled receptors-X1 (MRGPR-X1) are highly enriched in dorsal root ganglia (DRG) neurons and induce acute pain. Herein, we analyzed effects of MRGPR-X1 on serum response factors (SRF) or nuclear factors of activated
A Breit +73 more
core +24 more sources
Scalable Production of AAV Vectors in Orbitally Shaken HEK293 Cells [PDF]
Molecular Therapy: Methods & Clinical Development, 2019 Adeno-associated virus (AAV) vectors are currently among the most commonly applied for in vivo gene therapy approaches. The evaluation of vectors during clinical development requires the production of considerable amounts of highly pure and potent ...
Daniel Blessing +7 more
doaj +5 more sources
MicroRNA and Hemophilia-A Disease: Bioinformatics Prediction and Experimental Analysis [PDF]
Cell Journal, 2021 Objective: Hemophilia-A is a common genetic abnormality resulted from decreased or lack of factor VIII (FVIII) pro-coagulant protein function caused by mutations in the F8 gene.
Halimeh Rezaei +2 more
doaj +1 more source
The Inflammasome Activity of NLRP3 Is Independent of NEK7 in HEK293 Cells Co-Expressing ASC. [PDF]
Int J Mol Sci, 2022 Machtens DA +4 more
europepmc +2 more sources
Kidney Diseases, 2022 Introduction: Acute kidney injury (AKI) is a major public health problem worldwide. However, there is no definitive therapies to treat established AKI. In this study, we used FG-4592 to induce hypoxia inducible factor (HIF) expression in cells and then ...
Zhao-Ying Ding +7 more
doaj +1 more source
A Comparative Study on the Efficiency of CRISPR-Cas9 in Human Embryonic Kidney 293 Cells and Peripheral Blood Mononuclear Cells for Disruption in Programmed Cell Death Protein 1 [PDF]
Journal of Genetic Resources, 2022 CRISPR-Cas9 is the most important tool in genome engineering in recent years. The efficiency of this instrument on active and non-active genes is variable.
Seyed Ali Alambeladi +2 more
doaj +1 more source