Results 11 to 20 of about 4,870,076 (306)
Upregulation of PHLDA2 in Dicer knockdown HEK293 cells
Biochimica et Biophysica Acta (BBA) - General Subjects, 2007 It has been reported that RNAi-dependent chromatin silencing in vertebrates is not restricted to the centromeres. To address whether RNAi machinery could regulate the chromatin structure of imprinted genes, we knocked down Dicer in HEK293 cells and found that the expression of PHLDA2, one of the several genes in the imprinted gene domain of 11p15.5 ...
Kai-Fu, Tang +9 more
core +5 more sources
Functional Reconstruction of NLRs in HEK293 Cells
, 2016 Inflammasomes are molecular complexes that initiate innate immune response. They are mainly expressed by immune cells; however, molecular manipulations in these cells remain very difficult. Here, we describe a simple protocol to overexpress and activate functional NRLP3 inflammasomes in HEK293 cells.
Compan, Vincent, Lopez-Castejon, Gloria
core +6 more sources
Sodium Current Measurements in HEK293 Cells
Bio-Protocol, 2013 This protocol is used to measure sodium currents from heterologously transfected cells lines, such as HEK293 cells. Standard whole cell patch clamp technique is used to assess ion channel function. This protocol has been used to study cardiac type Nav1.5
Helena Riuró +5 more
doaj +2 more sources
Bio-Protocol, 2021 Recombinant proteins are an essential milestone for a plethora of different applications ranging from pharmaceutical to clinical, and mammalian cell lines are among the currently preferred systems to obtain large amounts of proteins of interest due to ...
Silvia Faravelli +5 more
doaj +2 more sources
Transient Gene Expression in Suspension HEK293-EBNA1 Cells
, 2018 Transient gene expression in human embryo kidney 293 (HEK293) cells is an established approach for the rapid production of large amounts of recombinant proteins (r-proteins). Milligram to gram quantities of r-proteins can be typically obtained within less than 10 days following transfection. In this chapter, we describe a simple and robust transfection
L’Abbé, Denis +4 more
openaire +3 more sources
VPS35 Deficiency Markedly Reduces the Proliferation of HEK293 Cells. [PDF]
Genes (Basel)Background/Objectives: The retromer protein complex is involved in various physiological processes, especially endosomal trafficking, and its dysregulation has been linked to Alzheimer’s disease and Parkinson’s disease, as well as VPS35 knockout (KO), causing early embryonic lethality.
Lee S +7 more
europepmc +3 more sources
Acta Virologica, 2023 Human adenovirus-5 (hAd5) is an important gene delivery vector, which has been widely used in various fields of biomedicine, such as gene therapy, cancer therapy, and vaccine development.
Wenbo Xie +4 more
doaj +1 more source
Characterization of amiodarone action on currents in hERG-T618 gain-of-function mutations
Open Life Sciences, 2023 Ami (10 μM) had a greater retarding effect on the hERG-T618I channel than WT (P < 0.05). The half-inhibitory concentration for the mutant (4.56 μM) was approximately 1.82 times that of WT (2.51 μM).
Lin Min +5 more
doaj +1 more source
Drug Delivery, 2023 Extracellular vesicles (EVs) are lipid membrane-bound particles involved in cell-to-cell communication through a delivery of regulatory molecules essential for physiological processes.
Stefania Zuppone +2 more
doaj +1 more source
Culture of HEK293-EBNA1 Cells for Production of Recombinant Proteins [PDF]
Cold Spring Harbor Protocols, 2008 INTRODUCTIONFast and efficient production of recombinant proteins (r-proteins) remains a major challenge for the academic and biopharmaceutical communities. Pure r-proteins are often required in large amounts (hundreds of milligrams to gram quantities) when being developed as biotherapeutics, or in smaller quantities (milligrams) for high-throughput ...
Roseanne, Tom +2 more
openaire +2 more sources