Results 1 to 10 of about 280,113 (289)
A Flow Cytometric Method to Determine Transfection Efficiency [PDF]
Bio-Protocol, 2019 Mammalian cell transfection is a powerful technique commonly used in molecular biology to express exogenous DNA or RNA in cells and study gene and protein function.
Wenli Mu +6 more
doaj +9 more sources
Assessment of Different Transfection Parameters in Efficiency Optimization
Cell Transplantation, 2004 Achieving optimal transfection efficiency is the most critical step in overcoming the primary obstacle to success in nonviral-mediated gene therapy.
A. T. L. Young +4 more
doaj +5 more sources
High-efficiency transfection of suspension cell lines [PDF]
BioTechniques, 2012 Transfection of suspension cells has proven to be very difficult using conventional methods. Here, we present a simple and time-saving new transfection protocol wherein cell culture plates coated with chicken egg white are seeded with suspension cells ...
Shereen Basiouni +2 more
doaj +4 more sources
Transfection efficiency boost of cholesterol-containing lipoplexes [PDF]
Biochimica Et Biophysica Acta - Biomembranes, 2012 Most lipid formulations require cholesterol for successful transfection, but the precise reason remains to be more clearly understood. Here, we have studied the effect of cholesterol on the transfection efficiency (TE) of lipoplexes in vitro. Addition of cholesterol to highly effective DC-Chol-DOPE/DNA lipoplexes increases TE, with 40 mol% cholesterol ...
Daniela Pozzi +2 more
exaly +9 more sources
The challenging nature of primary T lymphocytes for transfection: Effect of protamine sulfate on the transfection efficiency of chemical transfection reagents [PDF]
Research in Pharmaceutical Sciences, 2020 Background and purpose: The optimization of an effective non-viral gene delivery method for genetic manipulation of primary human T cells has been a major challenge in immunotherapy researches.
Ilnaz Rahimmanesh +2 more
doaj +5 more sources
Lipid-based Transfection Reagents Exhibit Cryo-induced Increase in Transfection Efficiency [PDF]
Molecular Therapy: Nucleic Acids, 2016 The advantages of lipid-based transfection reagents have permitted their widespread use in molecular biology and gene therapy. This study outlines the effect of cryo-manipulation of a cationic lipid-based formulation, Lipofectamine 2000, which, after ...
Helena Sork +17 more
doaj +3 more sources
ACS OmegaFor the process of transient transfection (TTF), DNA is often transported into the cells using polyplexes. The polyplex uptake and the subsequent transient expression of the gene of interest are of great importance for a successful transfection. In this study, we investigated a 3D-printed microfluidic system designed to facilitate direct TTF for ...
Michaela Dehne +7 more
doaj +3 more sources
BioTechniques, 2022 Gene manipulation in non-adhesive cells, especially lymphocytes, was difficult due to their low efficiency and high toxicity. Electroporation was reported as a highly efficient method for human and mouse lymphocytes.
Song Wang, Dan Tian
doaj +3 more sources
BioTechniques, 1996 A variety of methods have been described for introducing DNA into cultured mammalian cells. Among these, calcium phosphate (CaPO4)-mediated transfection has gained wide acceptance due to the procedure's low cost, simplicity and suitability for a wide ...
Guohong Zhang, Steven R. Kain
doaj +3 more sources
Cortisol increases transfection efficiency of cells [PDF]
FEBS Letters, 1997 DNA uptake can be facilitated by addition of physiological amounts of 11β‐hydroxy glucocorticosteroids (such as cortisol) during transfection. In the presence of cortisol, but not of the inactive 11‐keto glucocorticoid cortisone, twice as many cells uptake and express the reporter gene.
Bernasconi AG +5 more
openaire +5 more sources