Results 1 to 10 of about 3,929,037 (185)
High-Efficiency Transfection of Suspension Cell Lines
BioTechniques, 2012 Transfection of suspension cells has proven to be very difficult using conventional methods. Here, we present a simple and time-saving new transfection protocol wherein cell culture plates coated with chicken egg white are seeded with suspension cells ...
Herbert Fuhrmann +2 more
exaly +6 more sources
A Flow Cytometric Method to Determine Transfection Efficiency [PDF]
Bio-Protocol, 2019 Mammalian cell transfection is a powerful technique commonly used in molecular biology to express exogenous DNA or RNA in cells and study gene and protein function.
Wenli Mu +6 more
doaj +9 more sources
Assessment of Different Transfection Parameters in Efficiency Optimization
Cell Transplantation, 2004 Achieving optimal transfection efficiency is the most critical step in overcoming the primary obstacle to success in nonviral-mediated gene therapy.
Jonathan Rt Lakey +2 more
exaly +5 more sources
The challenging nature of primary T lymphocytes for transfection: Effect of protamine sulfate on the transfection efficiency of chemical transfection reagents [PDF]
Research in Pharmaceutical Sciences, 2020 Background and purpose: The optimization of an effective non-viral gene delivery method for genetic manipulation of primary human T cells has been a major challenge in immunotherapy researches.
Ilnaz Rahimmanesh +2 more
doaj +5 more sources
BioTechniques, 2022 Gene manipulation in non-adhesive cells, especially lymphocytes, was difficult due to their low efficiency and high toxicity. Electroporation was reported as a highly efficient method for human and mouse lymphocytes.
Song Wang, Dan Tian
doaj +3 more sources
Effect of Protein Corona on The Transfection Efficiency of Lipid-Coated Graphene Oxide-Based Cell Transfection Reagents [PDF]
Pharmaceutics, 2020 Coating graphene oxide nanoflakes with cationic lipids leads to highly homogeneous nanoparticles (GOCL NPs) with optimised physicochemical properties for gene delivery applications.
Erica Quagliarini +6 more
doaj +2 more sources
Gene delivery ability of polyethylenimine and polyethylene glycol dual-functionalized nanographene oxide in 11 different cell lines [PDF]
Royal Society Open Science, 2017 We recently developed a polyethylenimine (PEI) and polyethylene glycol (PEG) dual-functionalized reduced graphene oxide (GO) (PEG−nrGO−PEI, RGPP) for high-efficient gene delivery in HepG2 and Hela cell lines. To evaluate the feasibility and applicability
Liping Wu +6 more
doaj +1 more source
Pharmaceutics, 2022 The generation of reactive oxygen species (ROS) can affect cationic liposome-mediated transfection. In this study, we focused on a specific class of antioxidants, flavonoids, to investigate the transfection efficiency using cationic liposome/plasmid DNA ...
Die Hu +4 more
doaj +1 more source
Improving Ultrasound Gene Transfection Efficiency in Vitro [PDF]
Advanced Ultrasound in Diagnosis and Therapy, 2019 Objective: The purpose of this study was to optimize ultrasound-targeted microbubble destruction (UTMD) on RAGE plasmid transfection in human coronary artery endothelial cells (HCAECs) and improve gene transfection efficiency in vitro.Methods: SonoVue ...
Xianghong Luo, MD, Jianhui Zhang, MD, Sihui Shao, MD, Rong Wu, MD, Lianfang Du, MD, Jie Yuan, PhD, Zhaojun Li, MD
doaj +1 more source
BioTechniques, 2023 Transfection, a nonviral method of nucleic acid delivery, often exhibits poor efficiency in vivo. The needle-based in vivo delivery of transfection reagents can be invasive.
Nathan Liu +5 more
doaj +1 more source