Results 21 to 30 of about 3,929,037 (185)

Extracellular Nanovesicle Enhanced Gene Transfection Using Polyethyleneimine in HEK293T Cells and Zebrafish Embryos

open access: yesFrontiers in Bioengineering and Biotechnology, 2020
It is a hot topic to improve efficiency and decrease toxicity of gene transfection reagents. The extracellular nanovesicles (EVs) that are released by cells play an important role in intercellular communication and are naturally designed for genetic ...
Zhenzhen Zhang   +10 more
doaj   +1 more source

Efficient Nonviral Cutaneous Transfection

open access: yesMolecular Therapy, 2000
Preclinical in vivo rodent, porcine, and primate experiments aimed at enhancing nonviral transgene delivery to skin have been performed. These investigations have identified a compound (aurintricarboxylic acid or ATA) that enhances transfection activity of "naked" plasmid and pulsed electrical fields (electroporation or EP) that synergistically boosts ...
Glasspool-Malone, Jill   +3 more
openaire   +2 more sources

Tailoring optical fibers for cell transfection [PDF]

open access: yes, 2012
Optical transfection is a promising technique for the delivery of foreign genetic material into cells by transiently changing the permeability of the cell membrane.
Ma, Nan
core   +2 more sources

Fluorescence In Situ Hybridization Method for Measuring Transfection Efficiency

open access: yesBioTechniques, 1996
We describe here the use of fluorescence in situ hybridization (FISH) to measure the transfection efficiency of the transient expression vector pCMVcat in lymphoblasts and fibroblasts.
Lie Cheng, Corazon D. Bucana, Qingyi Wei
doaj   +1 more source

Laser-induced breakdown of an optically trapped gold nanoparticle for single cell transfection [PDF]

open access: yes, 2013
The cell selective introduction of therapeutic agents remains a challenging problem. Here we demonstrate spatially controlled cavitation instigated by laser-induced breakdown of an optically trapped single gold nanoparticle of diameter 100 nm. The energy
Gunn-Moore, Frank J   +12 more
core   +1 more source

Magnetofection potentiates gene delivery to cultured endothelial cells [PDF]

open access: yes, 2003
Modification of cellular functions by overexpression of genes is increasingly practised for research of signalling pathways, but restricted by limitations of low efficiency. We investigated whether the novel technique of magnetofection (MF) could enhance
Sohn, Hae-Young   +6 more
core   +1 more source

Supplementary Table 1 - High transfection efficiency and cell viability of immune cells with nanomaterials-based transfection reagent

open access: yes, 2022
High transfection efficiency and cell viability of immune cells with nanomaterials-based transfection reagent - Supplementary Table 1 Transfection efficiencies on day
Song Wang (144220), Dan Tian (697937)
core   +1 more source

Comparison of transfection efficiency of polymer-based and lipid-based transfection reagents

open access: yesBratislava Medical Journal, 2018
In this study, the optimal dose of Lipofectamine 3000 and Turbofect to transfect adherent cell lines such as CHO-K1 and HEK293 cells in comparison with non-adherent H9T-cells with pEGFP-N1 and pCDH was identified.Lipofectamine 3000 is a new transfection reagent which is claimed to be more efficient than other transfection reagents like Turbofect ...
P, Rahimi   +5 more
openaire   +3 more sources

Optical transfection and injection techniques applied to mammalian and embryonic cells [PDF]

open access: yes, 2012
The delivery of biomolecules into living cells is an important methodology in cell and molecular biology. Optical methods using lasers are attractive tools for such application.
Torres, Maria Leilani
core   +2 more sources

Hydrazone-modulated peptides for efficient gene transfection [PDF]

open access: yesJournal of Materials Chemistry B, 2017
The synthetic potential of dynamic bond formation is introduced for the delivery of plasmid DNA by modulated amphiphilic peptides. The synthetic advantage of these dynamic bonds allowed the identification of improved reagents (better efficiency and lower toxicity) for plasmid transfection assays in human HeLa cells.
Iria Louzao   +2 more
openaire   +3 more sources

Home - About - Disclaimer - Privacy