Results 21 to 30 of about 280,113 (289)

Extracellular Nanovesicle Enhanced Gene Transfection Using Polyethyleneimine in HEK293T Cells and Zebrafish Embryos

Frontiers in Bioengineering and Biotechnology, 2020
It is a hot topic to improve efficiency and decrease toxicity of gene transfection reagents. The extracellular nanovesicles (EVs) that are released by cells play an important role in intercellular communication and are naturally designed for genetic ...
Zhenzhen Zhang, Zhenzhen Zhang, Kai Wen, Chao Zhang, Fabrice Laroche, Zhenglong Wang, Qiang Zhou, Zunfeng Liu, Jan Pieter Abrahams, Xiang Zhou, Xiang Zhou   +10 more
doaj   +1 more source

Efficient Nonviral Cutaneous Transfection

Molecular Therapy, 2000
Preclinical in vivo rodent, porcine, and primate experiments aimed at enhancing nonviral transgene delivery to skin have been performed. These investigations have identified a compound (aurintricarboxylic acid or ATA) that enhances transfection activity of "naked" plasmid and pulsed electrical fields (electroporation or EP) that synergistically boosts ...
Glasspool-Malone, Jill, Somiari, Stella, Drabick, Joseph J., Malone, Robert W.   +3 more
openaire   +2 more sources

Fluorescence In Situ Hybridization Method for Measuring Transfection Efficiency

BioTechniques, 1996
We describe here the use of fluorescence in situ hybridization (FISH) to measure the transfection efficiency of the transient expression vector pCMVcat in lymphoblasts and fibroblasts.
Lie Cheng, Corazon D. Bucana, Qingyi Wei
doaj   +1 more source

Laser-induced breakdown of an optically trapped gold nanoparticle for single cell transfection [PDF]

, 2013
The cell selective introduction of therapeutic agents remains a challenging problem. Here we demonstrate spatially controlled cavitation instigated by laser-induced breakdown of an optically trapped single gold nanoparticle of diameter 100 nm. The energy
Gunn-Moore, Frank J, Arita, Yoshihiko, Antkowiak, Maciej, Ploschner, Martin, Dholakia, Kishan, Yoshihiko Arita, Maciej Antkowiak, Frank Gunn-Moore, Gunn-Moore, Frank, Martin Ploschner, Kishan Dholakia   +10 more
core   +1 more source

Incorporation of Indole Significantly Improves the Transfection Efficiency of Guanidinium-Containing Poly(methacrylamide)s [PDF]

, 2020
In this study, we report a highly efficient transfection agent based on terpolymer consisting of N-(2-hydroxypropyl)methacrylamide (HPMA), N-(3-guanidinopropyl) methacrylamide (GPMA), and N-(2-indolethyl)methacrylamide (IEMA) monomers by analogy to the ...
Leon, Zartner, Kalina, Peneva, Ceren, Cokca, Ilja, tabujew, Dagmar, Fischer   +4 more
core   +1 more source

Magnetofection potentiates gene delivery to cultured endothelial cells [PDF]

, 2003
Modification of cellular functions by overexpression of genes is increasingly practised for research of signalling pathways, but restricted by limitations of low efficiency. We investigated whether the novel technique of magnetofection (MF) could enhance
Sohn, Hae-Young, Gloe, Torsten, Krötz, Florian, Plank, Christian, Pohl, Ulrich   +4 more
core   +1 more source

Improvement of transcription- and transfection-efficiency by synthetic polyampholytes [PDF]

Nucleic Acids Symposium Series, 2008
Water-soluble PEG derivatives having both amino- and carboxyl-pendants (PEG-ACs) were synthesized, and examined for their transcription- and transfection-enhancing activity on DNA/polycation complexes. PEG-AC could be deposited onto the surface of DNA/polyethylenimine(PEI) complexes, and enhanced their transcriptional activity.
Chieko, Yoshihara, Tomoko, Ito, Chwen-Yang, Shew, Yoshiyuki, Koyama   +3 more
openaire   +2 more sources

A method for concentrating lipid peptide DNA and siRNA nanocomplexes that retains their structure and transfection efficiency. [PDF]

, 2015
Nonviral gene and small interfering RNA (siRNA) delivery formulations are extensively used for biological and therapeutic research in cell culture experiments, but less so in in vivo and clinical research.
Hart, S.L., Zhou, Haiyan, Munye, Mustafa M, Hart, Stephen L, Munye, MM, Munye MM, Tagalakis AD, Tagalakis, Aristides D, Tagalakis, Aristides, White, Edward A, White, EA, Castellaro, S, Zhou, H, Zhou H, Castellaro S, White EA, Bienemann, A, White, E.A., McCarthy, David, Munye, M.M., Castellaro, Sara, Tagalakis, AD, Hart, SL, Bienemann, Ali S, McCarthy, D, Bienemann A, Hart SL, McCarthy D   +27 more
core   +2 more sources

Poly(ethylene glycol)-Based Peptidomimetic “PEGtide” of Oligo-Arginine allows for efficient siRNA Transfection and gene inhibition [PDF]

, 2019
While a wide range of experimental and commercial transfection reagents are currently available, persistent problems remain regarding their suitability for continued development.
McCarthy, Joanna, Marc Devocelle (7895822), Forde, Éanna B., Joanna McCarthy (6818351), Gary Hessman (6818357), O'Driscoll, Caitríona M., Hibbitts, Alan, Devocelle, Marc, Cryan, Sally-Ann, O'Connor, Aoife M., Éanna B Forde (8009981), Hessman, Gary, Sally-Ann Cryan (7896101), Caitriona M O'Driscoll (8009984), Aoife M O'Connor (8009978), Alan Hibbitts (7895084)   +15 more
core   +1 more source

Comparison of transfection efficiency of polymer-based and lipid-based transfection reagents

Bratislava Medical Journal, 2018
In this study, the optimal dose of Lipofectamine 3000 and Turbofect to transfect adherent cell lines such as CHO-K1 and HEK293 cells in comparison with non-adherent H9T-cells with pEGFP-N1 and pCDH was identified.Lipofectamine 3000 is a new transfection reagent which is claimed to be more efficient than other transfection reagents like Turbofect ...
P, Rahimi, V Iranpur, Mobarakeh, S, Kamalzare, F, SajadianFard, R, Vahabpour, R, Zabihollahi   +5 more
openaire   +3 more sources