Results 11 to 20 of about 280,113 (289)

Femtosecond optical transfection of cells:viability and efficiency [PDF]

Optics Express, 2006
Photoporation is a rapidly expanding technique for the introduction of macromolecules into single cells. However, there remains no study into the true efficiency of this procedure. Here, we present a detailed analysis of transfection efficiency and cell viability for femtosecond optical transfection using a titanium sapphire laser at 800 nm ...
D, Stevenson, B, Agate, X, Tsampoula, P, Fischer, C T A, Brown, W, Sibbett, A, Riches, F, Gunn-Moore, K, Dholakia   +8 more
openaire   +3 more sources

Effect of Osmolarity and Presence of Serum on the Efficiency of Cell Transfection Using Immunoporation [PDF]

Analytical Cellular Pathology, 2001
Immunoporation is a novel method of cell transfection based upon the use of a new type of beads, Immunofect beads, that can be targeted to make holes in different types of cells depending on the type of bead used.
C. Tzavelas, P. Smith, E. Horefti, D. Rickwood   +3 more
doaj   +2 more sources

Effect of Protein Corona on The Transfection Efficiency of Lipid-Coated Graphene Oxide-Based Cell Transfection Reagents [PDF]

Pharmaceutics, 2020
Coating graphene oxide nanoflakes with cationic lipids leads to highly homogeneous nanoparticles (GOCL NPs) with optimised physicochemical properties for gene delivery applications.
Erica Quagliarini, Riccardo Di Santo, Sara Palchetti, Gianmarco Ferri, Francesco Cardarelli, Daniela Pozzi, Giulio Caracciolo   +6 more
doaj   +2 more sources

Transfection efficiency boost by designer multicomponent lipoplexes [PDF]

Biochimica et Biophysica Acta (BBA) - Biomembranes, 2007
Cationic liposome-DNA complexes (lipoplexes) have emerged as leading nonviral gene carriers in worldwide gene therapy clinical trials. Arriving at therapeutic dosages requires the full understanding of the mechanism of transfection. We investigated the correlation between structural evolution of multicomponent lipoplexes when interacting with cellular ...
CARACCIOLO, Giulio, POZZI, DANIELA, CAMINITI, Ruggero, Cristina Marchini, Maura Montani, Augusto Amici, Heinz Amenitsch   +6 more
core   +8 more sources

Efficient transfection of the archaebacterium Halobacterium halobium [PDF]

Journal of Bacteriology, 1987
We developed an efficient polyethylene glycol-mediated spheroplast transfection method for the extremely halophilic archaebacterium Halobacterium halobium. The 59-kilobase-pair linear phage phi H DNA molecule routinely produced between 5 X 10(6) and 2 X 10(7) transfectants per microgram of DNA.
S W, Cline, W F, Doolittle
openaire   +2 more sources

Efficient Transfection of Embryonic and Adult Stem Cells [PDF]

STEM CELLS, 2004
The ability of embryonic stem cells and adult stem cells to differentiate into specific cell types holds immense potential for therapeutic use in cell and gene therapy. Realization of this potential depends on efficient and optimized protocols for genetic manipulation of stem cells. In the study reported here, we demonstrate the use of nucleofection as
Lakshmipathy, Uma, Pelacho, Beatriz, Sudo, Kazuhiro, Linehan, Jonathan L., Coucouvanis, Electra, Kaufman, Dan S., Verfaillie, Catherine   +6 more
openaire   +2 more sources

Gene delivery ability of polyethylenimine and polyethylene glycol dual-functionalized nanographene oxide in 11 different cell lines [PDF]

Royal Society Open Science, 2017
We recently developed a polyethylenimine (PEI) and polyethylene glycol (PEG) dual-functionalized reduced graphene oxide (GO) (PEG−nrGO−PEI, RGPP) for high-efficient gene delivery in HepG2 and Hela cell lines. To evaluate the feasibility and applicability
Liping Wu, Jinshan Xie, Tan Li, Zihao Mai, Lu Wang, Xiaoping Wang, Tongsheng Chen   +6 more
doaj   +1 more source

Flavonoids Enhance Lipofection Efficiency and Ameliorate Cytotoxicity in Colon26 and HepG2 Cells via Oxidative Stress Regulation

Pharmaceutics, 2022
The generation of reactive oxygen species (ROS) can affect cationic liposome-mediated transfection. In this study, we focused on a specific class of antioxidants, flavonoids, to investigate the transfection efficiency using cationic liposome/plasmid DNA ...
Die Hu, Shintaro Fumoto, Hirotaka Miyamoto, Masakazu Tanaka, Koyo Nishida   +4 more
doaj   +1 more source

Improving Ultrasound Gene Transfection Efficiency in Vitro [PDF]

Advanced Ultrasound in Diagnosis and Therapy, 2019
Objective: The purpose of this study was to optimize ultrasound-targeted microbubble destruction (UTMD) on RAGE plasmid transfection in human coronary artery endothelial cells (HCAECs) and improve gene transfection efficiency in vitro.Methods: SonoVue ...
Xianghong Luo, MD, Jianhui Zhang, MD, Sihui Shao, MD, Rong Wu, MD, Lianfang Du, MD, Jie Yuan, PhD, Zhaojun Li, MD
doaj   +1 more source

A novel use of a needle-free injection system for improved nucleic acid delivery and expression in vivo

BioTechniques, 2023
Transfection, a nonviral method of nucleic acid delivery, often exhibits poor efficiency in vivo. The needle-based in vivo delivery of transfection reagents can be invasive.
Nathan Liu, Hani Abd-Ul-Salam, Noémie Joannette-Lafrance, Jingjing Li, Karim Menassa, Monzur Murshed   +5 more
doaj   +1 more source