Results 11 to 20 of about 3,929,037 (185)

Lipid-based Transfection Reagents Exhibit Cryo-induced Increase in Transfection Efficiency [PDF]

Molecular Therapy: Nucleic Acids, 2016
The advantages of lipid-based transfection reagents have permitted their widespread use in molecular biology and gene therapy. This study outlines the effect of cryo-manipulation of a cationic lipid-based formulation, Lipofectamine 2000, which, after ...
Helena Sork, Joel Z Nordin, Janne J Turunen, Oscar PB Wiklander, Burcu Bestas, Eman M Zaghloul, Helerin Margus, Kärt Padari, Adil D Duru, Giulia Corso, Jeremy Bost, Pieter Vader, Margus Pooga, CI Edvard Smith, Matthew JA Wood, Raymond M Schiffelers, Mattias Hällbrink, Samir EL Andaloussi   +17 more
doaj   +3 more sources

Cortisol increases transfection efficiency of cells [PDF]

FEBS Letters, 1997
DNA uptake can be facilitated by addition of physiological amounts of 11β‐hydroxy glucocorticosteroids (such as cortisol) during transfection. In the presence of cortisol, but not of the inactive 11‐keto glucocorticoid cortisone, twice as many cells uptake and express the reporter gene.
Bernasconi AG, Rebuffat AG, Lovati E, Frey BM, Frey FJ, Galli I   +5 more
openaire   +4 more sources

Transfection Maximizer Increases the Efficiency of Calcium Phosphate Transfections with Mammalian Cells

BioTechniques, 1996
A variety of methods have been described for introducing DNA into cultured mammalian cells. Among these, calcium phosphate (CaPO4)-mediated transfection has gained wide acceptance due to the procedure's low cost, simplicity and suitability for a wide ...
Guohong Zhang, Steven R. Kain
doaj   +3 more sources

Effect of Osmolarity and Presence of Serum on the Efficiency of Cell Transfection Using Immunoporation [PDF]

Analytical Cellular Pathology, 2001
Immunoporation is a novel method of cell transfection based upon the use of a new type of beads, Immunofect beads, that can be targeted to make holes in different types of cells depending on the type of bead used.
C. Tzavelas, P. Smith, E. Horefti, D. Rickwood   +3 more
doaj   +2 more sources

Microfluidic Transfection System and Temperature Strongly Influence the Efficiency of Transient Transfection

ACS Omega
For the process of transient transfection (TTF), DNA is often transported into the cells using polyplexes. The polyplex uptake and the subsequent transient expression of the gene of interest are of great importance for a successful transfection. In this study, we investigated a 3D-printed microfluidic system designed to facilitate direct TTF for ...
Michaela Dehne, Simon Valentin Neidinger, Michael Stark, Antonia Camilla Adamo, Xenia Kraus, Nicolas Färber, Christoph Westerhausen, Janina Bahnemann   +7 more
doaj   +3 more sources

Transfection efficiency boost by designer multicomponent lipoplexes [PDF]

Biochimica et Biophysica Acta (BBA) - Biomembranes, 2007
Cationic liposome-DNA complexes (lipoplexes) have emerged as leading nonviral gene carriers in worldwide gene therapy clinical trials. Arriving at therapeutic dosages requires the full understanding of the mechanism of transfection. We investigated the correlation between structural evolution of multicomponent lipoplexes when interacting with cellular ...
CARACCIOLO, Giulio, POZZI, DANIELA, CAMINITI, Ruggero, Cristina Marchini, Maura Montani, Augusto Amici, Heinz Amenitsch   +6 more
openaire   +5 more sources

Transfection efficiency of lipoplexes for site-directed delivery

Journal of Liposome Research, 2009
Targeted gene delivery is a promising strategy to cure disease on its basic level at the site of interest. The ultrastructure, internalization, and transfection efficiency of lipoplexes was investigated. We found that at a charge ratio (rho) of 4.0 lipoplexes had optimum characteristics for gene delivery in vitro. To decrease the size of lipoplexes, we
Jellema, Reint K., Bomans, Paul, Deckers, Niko, Ungethum, Liset, Reutelingsperger, Chris P. M., Hofstra, Leo, Frederik, Peter M.   +6 more
openaire   +4 more sources

Efficient transfection of the archaebacterium Halobacterium halobium [PDF]

Journal of Bacteriology, 1987
We developed an efficient polyethylene glycol-mediated spheroplast transfection method for the extremely halophilic archaebacterium Halobacterium halobium. The 59-kilobase-pair linear phage phi H DNA molecule routinely produced between 5 X 10(6) and 2 X 10(7) transfectants per microgram of DNA.
S W, Cline, W F, Doolittle
openaire   +2 more sources

Efficient Transfection of Embryonic and Adult Stem Cells [PDF]

STEM CELLS, 2004
The ability of embryonic stem cells and adult stem cells to differentiate into specific cell types holds immense potential for therapeutic use in cell and gene therapy. Realization of this potential depends on efficient and optimized protocols for genetic manipulation of stem cells. In the study reported here, we demonstrate the use of nucleofection as
Lakshmipathy, Uma, Pelacho, Beatriz, Sudo, Kazuhiro, Linehan, Jonathan L., Coucouvanis, Electra, Kaufman, Dan S., Verfaillie, Catherine   +6 more
openaire   +2 more sources

Optical sorting and photo-transfection of mammalian cells [PDF]

, 2010
Recently, laser light sources of different regimes have emerged as an essential tool in the biophotonics research area. Classic applications include, for example: manipulating single cells and their subcellular organelles, sorting cells in microfluidic ...
Mthunzi, Patience
core   +2 more sources