Results 31 to 40 of about 280,113 (289)
High-efficiency formation of influenza virus transfectants [PDF]
Journal of Virology, 1991 cDNA-derived RNAs were introduced into the genomes of influenza viruses by using an improved ribonucleoprotein (RNP) transfection protocol. Up to 10(5) viral transfectants with a novel neuraminidase gene could be obtained by using a 35-mm dish (10(6) cells) for RNP transfection.
M, Enami, P, Palese
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Hydrazone-modulated peptides for efficient gene transfection [PDF]
Journal of Materials Chemistry B, 2017 The synthetic potential of dynamic bond formation is introduced for the delivery of plasmid DNA by modulated amphiphilic peptides. The synthetic advantage of these dynamic bonds allowed the identification of improved reagents (better efficiency and lower toxicity) for plasmid transfection assays in human HeLa cells.
Iria Louzao +2 more
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Divalent Metal Ions Boost Effect of Nucleic Acids Delivered by Cell-Penetrating Peptides
Cells, 2022 Cell-penetrating peptides (CPPs) are promising tools for the transfection of various substances, including nucleic acids, into cells. The aim of the current work was to search for novel safe and effective approaches for enhancing transfection efficiency ...
Maria Maloverjan +4 more
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Journal of Medical Sciences, 2015 Background: In our previous study, HSA-PEI demonstrated high pDNA transfection efficiency and low cytotoxicity. Materials and Methods: In this study, the relationship between albumin receptors and the high pDNA transfection efficiency of HSA-PEI was ...
Ssu-Wei Peng +4 more
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Efficient transfection of primary zebrafish fibroblasts by nucleofection [PDF]
Cytotechnology, 2006 Although various gene delivery techniques are available, their application in zebrafish cell cultures has not been extensively studied. Here, we report that nucleofection of zebrafish primary embryonic fibroblasts results in higher transfection efficiency in comparison to other non-viral gene delivery methods. The transfection was performed using green
Rossen, Badakov, Anna, Jaźwińska
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Exploitation of De Novo Helper-Lipids for Effective Gene Delivery.
Journal of Pharmacy & Pharmaceutical Sciences, 2009 Purpose: In gene delivery, a fusogenic lipid such as dioleyl phosphatidylethanolamine (DOPE) which is a component of cationic liposomal vector is important factor for effective transfection efficiency. We investigated the effect of penetration enhancers
Tomoaki Kurosaki +9 more
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The effect of environmental pH on polymeric transfection efficiency [PDF]
Biomaterials, 2012 Although polymers, polyplexes, and cells are exposed to various extracellular and intracellular pH environments during polyplex preparation and polymeric transfection, the impact of environmental pH on polymeric transfection has not yet been investigated.
Han Chang, Kang +3 more
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Transfection efficiency in SV-LECs. [PDF]
, 2016 SV-LECs were transfected with control vector (pcDNA) or a green fluorescence protein expression vector pEGFP as described in the “Materials and methods” section. After transfection, cells were harvested and resuspended in PBS.
Ming-Jen Hsu (490331) +5 more
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Cationic amphiphiles based on malonic acid amides as transfection mediators
Тонкие химические технологии, 2020 Objectives. The aim of this work is to synthesize cationic amphiphiles based on malonic acid amides. The target compounds should contain saturated and unsaturated alkyl chains in the hydrophobic portion, and one or two positive charges in the polar head ...
N. A. Romanova +2 more
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Electroporation for the efficient transfection of mammalian cells with DNA [PDF]
Nucleic Acids Research, 1987 A simple and reproducible procedure for the introduction of DNA into mammalian cells by electroporation is described. The parameters involving the cells, the DNA, and the electric field are investigated. The procedure has been applied to a broad range of animal cells.
G, Chu, H, Hayakawa, P, Berg
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