Results 31 to 40 of about 3,929,037 (185)

Divalent Metal Ions Boost Effect of Nucleic Acids Delivered by Cell-Penetrating Peptides

Cells, 2022
Cell-penetrating peptides (CPPs) are promising tools for the transfection of various substances, including nucleic acids, into cells. The aim of the current work was to search for novel safe and effective approaches for enhancing transfection efficiency ...
Maria Maloverjan, Kärt Padari, Aare Abroi, Ana Rebane, Margus Pooga   +4 more
doaj   +1 more source

Improvement of transcription- and transfection-efficiency by synthetic polyampholytes [PDF]

Nucleic Acids Symposium Series, 2008
Water-soluble PEG derivatives having both amino- and carboxyl-pendants (PEG-ACs) were synthesized, and examined for their transcription- and transfection-enhancing activity on DNA/polycation complexes. PEG-AC could be deposited onto the surface of DNA/polyethylenimine(PEI) complexes, and enhanced their transcriptional activity.
Chieko, Yoshihara, Tomoko, Ito, Chwen-Yang, Shew, Yoshiyuki, Koyama   +3 more
openaire   +2 more sources

The mechanism of high transfection efficiency of human serum albumin conjugated polyethylenimine in A549 cells

Journal of Medical Sciences, 2015
Background: In our previous study, HSA-PEI demonstrated high pDNA transfection efficiency and low cytotoxicity. Materials and Methods: In this study, the relationship between albumin receptors and the high pDNA transfection efficiency of HSA-PEI was ...
Ssu-Wei Peng, Wei-Hsu Ko, Ming-Kung Yeh, Chiao-Hsi Chiang, Jiin-Long Chen   +4 more
doaj   +1 more source

A method for concentrating lipid peptide DNA and siRNA nanocomplexes that retains their structure and transfection efficiency. [PDF]

, 2015
Nonviral gene and small interfering RNA (siRNA) delivery formulations are extensively used for biological and therapeutic research in cell culture experiments, but less so in in vivo and clinical research.
Hart, S.L., Zhou, Haiyan, Munye, Mustafa M, Hart, Stephen L, Munye, MM, Munye MM, Tagalakis AD, Tagalakis, Aristides D, Tagalakis, Aristides, White, Edward A, White, EA, Castellaro, S, Zhou, H, Zhou H, Castellaro S, White EA, Bienemann, A, White, E.A., McCarthy, David, Munye, M.M., Castellaro, Sara, Tagalakis, AD, Hart, SL, Bienemann, Ali S, McCarthy, D, Bienemann A, Hart SL, McCarthy D   +27 more
core   +1 more source

Exploitation of De Novo Helper-Lipids for Effective Gene Delivery.

Journal of Pharmacy & Pharmaceutical Sciences, 2009
Purpose: In gene delivery, a fusogenic lipid such as dioleyl phosphatidylethanolamine (DOPE) which is a component of cationic liposomal vector is important factor for effective transfection efficiency. We investigated the effect of penetration enhancers
Tomoaki Kurosaki, Takashi Kitahara, Mugen Teshima, Koyo Nishida, Junzo Nakamura, Mikiro Nakashima, Hideto To, Hiromitsu Hukuchi, Tomoyuki Hamamoto, Hitoshi Sasaki   +9 more
doaj   +1 more source

Cationic amphiphiles based on malonic acid amides as transfection mediators

Тонкие химические технологии, 2020
Objectives. The aim of this work is to synthesize cationic amphiphiles based on malonic acid amides. The target compounds should contain saturated and unsaturated alkyl chains in the hydrophobic portion, and one or two positive charges in the polar head ...
N. A. Romanova, U. A. Budanova, Yu. L. Sebyakin   +2 more
doaj   +1 more source

Femtosecond optical transfection of cells:viability and efficiency

Optics Express, 2006
Photoporation is a rapidly expanding technique for the introduction of macromolecules into single cells. However, there remains no study into the true efficiency of this procedure. Here, we present a detailed analysis of transfection efficiency and cell viability for femtosecond optical transfection using a titanium sapphire laser at 800 nm ...
D, Stevenson, B, Agate, X, Tsampoula, P, Fischer, C T A, Brown, W, Sibbett, A, Riches, F, Gunn-Moore, K, Dholakia   +8 more
openaire   +2 more sources

Efficient transfection of primary zebrafish fibroblasts by nucleofection [PDF]

Cytotechnology, 2006
Although various gene delivery techniques are available, their application in zebrafish cell cultures has not been extensively studied. Here, we report that nucleofection of zebrafish primary embryonic fibroblasts results in higher transfection efficiency in comparison to other non-viral gene delivery methods. The transfection was performed using green
Rossen, Badakov, Anna, Jaźwińska
openaire   +2 more sources

Characterization of cationic lipid DNA transfection complexes differing in susceptability to serum inhibition

BMC Biotechnology, 2002
Background Cationic lipid DNA complexes based on DOTAP (1,2-dioleoyl-3-(trimethyammonium) propane) and mixtures of DOTAP and cholesterol (DC) have been previously optimized for transfection efficiency in the absence of serum and used as a non-viral gene ...
Überla Klaus, Nchinda Godwin, Zschörnig Olaf   +2 more
doaj   +1 more source

The Prodigious Potential of mRNA Electrotransfer as a Substitute to Conventional DNA-Based Transient Transfection

Cells, 2023
Transient transfection of foreign DNA is the most widely used laboratory technique to study gene function and product. However, the transfection efficiency depends on many parameters, including DNA quantity and quality, transfection methods and target ...
Théo Juncker, Bruno Chatton, Mariel Donzeau   +2 more
doaj   +1 more source