Results 51 to 60 of about 3,929,037 (185)

Genosomes: An Introspection into Transfection, Future Perspectives and Applications [PDF]

Advanced Pharmaceutical Bulletin
The advent of advanced gene delivery platforms has transformed the precision targeting of therapeutic nucleic acids, such as miRNA, siRNA, and pDNA, for the treatment of genetic and acquired diseases, including cystic fibrosis, malignancies, sickle cell ...
Abhirami Subramony, Divyatha Raj, Fiza Fairooz, Aparna Venugopal, Aparna Rajesh, Sreeja C Nair   +5 more
doaj   +1 more source

Electroporation for the efficient transfection of mammalian cells with DNA [PDF]

Nucleic Acids Research, 1987
A simple and reproducible procedure for the introduction of DNA into mammalian cells by electroporation is described. The parameters involving the cells, the DNA, and the electric field are investigated. The procedure has been applied to a broad range of animal cells.
G, Chu, H, Hayakawa, P, Berg
openaire   +2 more sources

Transfection efficiency in SV-LECs.

, 2016
SV-LECs were transfected with control vector (pcDNA) or a green fluorescence protein expression vector pEGFP as described in the “Materials and methods” section. After transfection, cells were harvested and resuspended in PBS.
Ming-Jen Hsu (490331), Yu-Han Huang (276128), George Ou (490330), Shiu-Wen Huang (789688), Ya-Fen Hsu (490324), Hung-Yu Yang (490323)   +5 more
core   +1 more source

Fully Integrated Self-Powered Electrical Stimulation Cell Culture Dish for Noncontact High-Efficiency Plasmid Transfection

, 2021
Plasmid DNA transfection of mammalian cells is widely used in biomedical research and genetic drug delivery, but low transfection efficiency, especially in the context of the primary cells, limits its application.
Youbin Zheng, Miaomiao Yuan, Ning Tang, Xuemei Fu, Ning Tang (138218), Rongjun Zhang, Hossam Haick (1279257), Shuxiang Xu (11676219), Li Cheng (228291), Xiaoying Li (147528), Jingfeng Liu (1392460), Rongjun Zhang (2359339), Yang Xu, Yang Xu (178421), Jingfeng Liu, Li Cheng, Shuxiang Xu, Miaomiao Yuan (2005606), Xiaoying Li, Xuemei Fu (5549207), Youbin Zheng (8442225), Hossam Haick   +21 more
core   +1 more source

Comparing chemical transfection, electroporation, and lentiviral vector transduction to achieve optimal transfection conditions in the Vero cell line

BMC Molecular and Cell Biology
Background Transfection is an important analytical method for studying gene expression in the cellular environment. There are some barriers to efficient DNA transfection in host cells, including circumventing the plasma membrane, escaping endosomal ...
Parisa Jamour, Abbas Jamali, Arash Ghalyanchi Langeroudi, Behrouz Ebadi Sharafabad, Asghar Abdoli   +4 more
doaj   +1 more source

Comparison of different kinds of nonviral vectors for gene delivery to human periodontal ligament stem cells

Journal of Dental Sciences, 2015
Background/purpose: Inducing human-periodontal-ligament-stem-cell (hPDLSC) differentiation by DNA transfection is a promising way for periodontal tissue engineering. However, there are only a few studies that focus on the introduction of foreign DNA into
Yajing Wang, Shuhuan Shang, Chengzhang Li   +2 more
doaj   +1 more source

Electroporation of mouse embryonic stem cells with Neon device

Ветеринария сегодня, 2022
Mouse embryonic stem cells are widely used as a promising material for producing of new cellular systems with desired properties in cellular and molecularbiology, pharmacology, virology, medicine, veterinary medicine and biotechnology. Each type of cells
I. P. Savchenkova, A. A. Savchenkova
doaj   +1 more source

Primary Neuronal Culture and Transient Transfection

Bio-Protocol
Primary neuronal culture and transient transfection offer a pair of crucial tools for neuroscience research, providing a controlled environment to study the behavior, function, and interactions of neurons in vitro.
Shun- Tseng, Peng- Chen, Eric Hwang
doaj   +1 more source

Efficient Transfection of siRNA by Peptide Dendrimer–Lipid Conjugates [PDF]

ChemBioChem, 2016
AbstractEfficient delivery of small interfering RNA (siRNA) into cells is the basis of target‐gene‐specific silencing and, ultimately, gene therapy. However, current transfection reagents are relatively inefficient, and very few studies provide the sort of systematic understanding based on structure–activity relationships that would provide rationales ...
Albert Kwok, Gabriela A. Eggimann, Marc Heitz, Jean‐Louis Reymond, Florian Hollfelder, Tamis Darbre   +5 more
openaire   +4 more sources

Enhancement of transfection efficiency with NLS and SPB-NLS

Molecular Medicine Reports, 2014
Low positive cell screening efficiency severely hinders the development of transgenic animals. The major rate-limiting step of positive cell screening is DNA entering the nucleus, particularly for large DNA molecules. To enhance the transport of large DNA molecules into the nucleus, particularly for the production of transgenic animals, nuclear ...
Jian, Lin, Tao, Qin, Li Qi, Zhu, Qiang, Zhang, Qing Hua, Yu, Qian, Yang   +5 more
openaire   +3 more sources