Gene transfection efficiency of OTMCS-PEI-R18/DNA polyplexes.
(A) Representative fluorescence images for transfection in Hela cells using OTMCS-PEI-R18 at w/w ratios of 5, 10, 20, and 30. (B) Transfection efficiency of OTMCS-PEI-R18/DNA complexes in Hela cell line at w/w ratios of 5, 10, 20, and 30.
Yuan Mao (403724) +6 more
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Evaluation of Transfected HEP-2 Cell Line Using ß-Galactosidase Reporter Assay System
Liposome-mediated transfection of cancer cells provide a valuable experimental technique to study cellular gene expression and may also be adapted for gene therapy studies.
Baghdad Science Journal
doaj +1 more source
The spatial orientation of histidine via five-armed alkylamino siloxane improved the properties of the cationic gene delivery vector [PDF]
The efficiency of gene transfection using cationic polymers primarily depends on factors such as compact polyplex formation, cellular uptake, endosomal escape, cytoplasmic transport, nuclear entry, and the dissociation and release of plasmid from the ...
Viola B. Morris, Chandra P. Sharma
doaj +1 more source
Enhancement of the efficiency of femtosecond optical transfection
Cell transfection is the process in which extra cellular nucleic acids such as DNA, RNA, Si-RNA can be deliberately injected into the cytoplasm of the cell.
Bavishna B. Praveen +10 more
core +1 more source
Optimization of Calcium Phosphate Transfection Protocol
Introduction. Despite the advancement of modern transfection methods, such as the use of polyethylenimine- and Lipofectamine-based reagents, their high cost limits their application in large-scale research and biotechnological processes.
Amina M. M. Al-Khadj Aioub +3 more
doaj +1 more source
A Highly Efficient siRNA Transfection Method in Primary Cultured Cortical Neurons
Transfecting neurons remains technically challenging due to their sensitivity. Conventional methods, such as Lipofectamine 2000 or Lipofectamine RNAiMAX, often result in significant cytotoxicity, which limits their utility.
Xiaorong Wang +4 more
doaj +1 more source
Transient transfection of Cryptosporidium parvum using green fluorescent protein (GFP) as a marker [PDF]
Cryptosporidium parvum is a protozoan parasite that infects a variety of mammals. The parasite has been shown to harbor a dsRNA virus (CPV) and in the present study, we have developed a CPV transient transfection system for this parasite by using green ...
Yu, X. +8 more
core
pH dependent high transfection efficiency of mouse neuroblastomas using TransFectin
pH dependent high transfection efficiency of mouse neuroblastomas using ...
S Seet (13295490) +4 more
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High Transfection Efficiency of Neural Stem Cells with Magnetofection
Primary neural stem cells (NSCs) can be cultivated and differentiated in vitro but are difficult to transfect using conventional methods. We describe a simple and rapid magnetofection-based method suitable for the lab bench as well as for high-throughput projects.
Cédric Sapet +6 more
openaire +3 more sources
Fatemeh Nouri,1 Hossein Sadeghpour,2 Reza Heidari,3 Ali Dehshahri,1,4,5 1Department of Pharmaceutical Biotechnology, 2Department of Medicinal Chemistry, 3Department of Pharmacology and Toxicology, School of Pharmacy, 4Center for Nanotechnology in Drug ...
Dehshahri A +3 more
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