Results 61 to 70 of about 280,113 (289)
Transfection efficiency of rE.mi of different passages. [PDF]
, 2014 Transfection efficiency of rE.mi of different passages.
Xun Suo (317782) +5 more
core +1 more source
Optimization of chemical transfection in airway epithelial cell lines
BMC BiotechnologyBackground Chemical transfection is a widely employed technique in airway epithelium research, enabling the study of gene expression changes and effects. Additionally, it has been explored for its potential application in delivering gene therapies. Here,
Tony J. F. Guo +5 more
doaj +1 more source
Efficient Transfection of siRNA by Peptide Dendrimer–Lipid Conjugates [PDF]
ChemBioChem, 2016 AbstractEfficient delivery of small interfering RNA (siRNA) into cells is the basis of target‐gene‐specific silencing and, ultimately, gene therapy. However, current transfection reagents are relatively inefficient, and very few studies provide the sort of systematic understanding based on structure–activity relationships that would provide rationales ...
Albert Kwok +5 more
openaire +4 more sources
Continuous monitoring of cell transfection efficiency with micropatterned substrates
Biotechnology and Bioengineering, 2021 AbstractThe effect of cell–cell contact on gene transfection is mainly unknown. Usually, transfection is carried out in batch cell cultures without control over cellular interactions, and efficiency analysis relies on complex and expensive protocols commonly involving flow cytometry as the final analytical step.
Enrique Azuaje‐Hualde +5 more
openaire +3 more sources
The role of miR‐335‐5p in the redifferentiation of BRAF p.V600E thyroid cancers
Molecular Oncology, EarlyView.The BRAF p.V600E mutation promotes thyroid cancer dedifferentiation and radioiodine resistance. Using a network approach, we identified miR‐335‐5p as a key regulator of BRAF‐mutated thyroid tumors. Restoring miR‐335‐5p increased thyroid‐specific gene expression and iodine uptake in cells and organoids.
Valeria Pecce +11 more
wiley +1 more source
Optical transfection and injection techniques applied to mammalian and embryonic cells [PDF]
, 2012 The delivery of biomolecules into living cells is an important methodology in cell and molecular biology. Optical methods using lasers are attractive tools for such application.
Torres, Maria Leilani
core
Genosomes: An Introspection into Transfection, Future Perspectives and Applications [PDF]
Advanced Pharmaceutical BulletinThe advent of advanced gene delivery platforms has transformed the precision targeting of therapeutic nucleic acids, such as miRNA, siRNA, and pDNA, for the treatment of genetic and acquired diseases, including cystic fibrosis, malignancies, sickle cell ...
Abhirami Subramony +5 more
doaj +1 more source
Exploration of high-efficiency transfection methods for sheep fibroblasts OAR-L1
浙江大学学报. 农业与生命科学版, 2022 In order to achieve high-efficiency expression of exogenous protein in sheep lung fibroblasts OAR-L1, and to explore a suitable transfection method for the cell line, the transfection efficiencies of polyethyleneimine (PEI), LipofectamineTM 2000 ...
WU Fei +9 more
doaj +1 more source
Molecular Oncology, EarlyView.Keratin 19 (KRT19) is overexpressed in high‐grade serous ovarian cancer with high levels of Kallikrein‐related peptidases (KLK) 4–7 and is associated with poor survival. In vivo analyses demonstrate that elevated KRT19 increases peritoneal tumour burden.
Sophia Bielesch +13 more
wiley +1 more source
Use of Primary and Secondary Polyvinylamines for Efficient Gene Transfection [PDF]
Biomacromolecules, 2017 Gene transfection with polymeric carrier remains a challenge; particularly, high transfection levels combined with low toxicity are hard to achieve. We herein revisit polyvinylamines, an old and neglected family of cationic polymers. They can be readily obtained by controlled hydrolysis of polyvinylamides prepared through (controlled) radical ...
Dréan, Mathilde +6 more
openaire +3 more sources