Results 61 to 70 of about 3,929,037 (185)

Demethylation using the epigenetic modifier, 5-azacytidine, increases the efficiency of transient transfection of macrophages

Journal of Lipid Research, 2005
This study was aimed at developing a method for high-efficiency transient transfection of macrophages. Seven methods were evaluated for transient transfection of murine macrophage RAW 264.7 cells.
Geneviève Escher, Anh Hoang, Suzan Georges, Urbain Tchoua, Assam El-Osta, Zygmunt Krozowski, Dmitri Sviridov   +6 more
doaj   +1 more source

Combinational use of lipid-based reagents for efficient transfection of primary fibroblasts and hepatoblasts

BioTechniques, 2017
Commercially available lipid-based transfection reagents are widely used to deliver DNA to cells. However, these lipid-based transfection reagents show poor gene transfer efficiency in primary cells.
Kazuhiro Ishiguro, Osamu Watanabe, Masanao Nakamura, Takeshi Yamamura, Masanobu Matsushita, Hidemi Goto, Yoshiki Hirooka   +6 more
doaj   +1 more source

An improved method for the rescue of recombinant Newcastle disease virus

BioTechniques, 2020
Reverse genetics has been used to generate recombinant Newcastle disease virus with enhanced immunogenic properties for vaccine development. The system, which involves co-transfecting the viral antigenomic plasmid with three helper plasmids into a T7 RNA
Pheik-Sheen Cheow, Tiong Kit Tan, Adelene Ai-Lian Song, Khatijah Yusoff, Suet Lin Chia   +4 more
doaj   +1 more source

Use of Primary and Secondary Polyvinylamines for Efficient Gene Transfection [PDF]

Biomacromolecules, 2017
Gene transfection with polymeric carrier remains a challenge; particularly, high transfection levels combined with low toxicity are hard to achieve. We herein revisit polyvinylamines, an old and neglected family of cationic polymers. They can be readily obtained by controlled hydrolysis of polyvinylamides prepared through (controlled) radical ...
Dréan, Mathilde, Debuigne, Antoine, Gonçalves, Cristine, Jérôme, Christine, Midoux, Patrick, Rieger, Jutta, Guegan, Philippe   +6 more
openaire   +3 more sources

Transfection efficiency of PEG-Bu.

, 2013
Transfection efficiency of PEG-Bu/pGL3-Control complexes at various w/w ratios in serum–free media, and transfection efficiency of PEG-Bu/pGL3-Control complexes (at optimal w/w) in serum-containing (10%) media in (A, C) BRL-3A, and (B, D) HeLa cell lines
Shu-Yan Chen (435261), Yu-Qiang Wang (435258), Jing Su (23377), Jing Sheng (435260), Fei Wang (19534), Xiao-Qin Deng (435259)   +5 more
core   +1 more source

A novel rapid and reproducible flow cytometric method for optimization of transfection efficiency in cells.

PLoS ONE, 2017
Transfection is one of the most frequently used techniques in molecular biology that is also applicable for gene therapy studies in humans. One of the biggest challenges to investigate the protein function and interaction in gene therapy studies is to ...
Stefanie Homann, Christian Hofmann, Aleksandr M Gorin, Huy Cong Xuan Nguyen, Diana Huynh, Phillip Hamid, Neil Maithel, Vahe Yacoubian, Wenli Mu, Athanasios Kossyvakis, Shubhendu Sen Roy, Otto Orlean Yang, Theodoros Kelesidis   +12 more
doaj   +1 more source

Efficient siRNA delivery and gene silencing using a lipopolypeptide hybrid vector mediated by a caveolae-mediated and temperature-dependent endocytic pathway

Journal of Nanobiotechnology, 2019
Background We developed a non-viral vector, a combination of HIV-1 Tat peptide modified with histidine and cysteine (mTat) and polyethylenimine, jetPEI (PEI), displaying the high efficiency of plasmid DNA transfection with little toxicity.
Hironori Kasai, Kenji Inoue, Kentaro Imamura, Carlo Yuvienco, Jin K. Montclare, Seiichi Yamano   +5 more
doaj   +1 more source

In vitro transfection efficiency of CP/DNA nanoparticles.

, 2014
(a) In vitro transfection efficiency of CP/DNA nanoparticles in both chondrocytes and synoviocytes compared to that of naked pDNA, CS/DNA nanoparticles, PEI (25 kDa)/DNA nanoparticles, and Lipofectamine™ 2000 (n = 3; 48 h post-transfection; error bars ...
Huading Lu (5520116), Huiqing Zhao (436302), Yuhu Dai (436301), Lulu Lv (436300)   +3 more
core   +1 more source

Incorporation of Indole Significantly Improves the Transfection Efficiency of Guanidinium-Containing Poly(methacrylamide)s [PDF]

, 2020
In this study, we report a highly efficient transfection agent based on terpolymer consisting of N-(2-hydroxypropyl)methacrylamide (HPMA), N-(3-guanidinopropyl) methacrylamide (GPMA), and N-(2-indolethyl)methacrylamide (IEMA) monomers by analogy to the ...
Leon, Zartner, Kalina, Peneva, Ceren, Cokca, Ilja, tabujew, Dagmar, Fischer   +4 more
core   +2 more sources

Use of Flow Cytometry to Rapidly Optimize the Transfection of Animal Cells

BioTechniques, 2000
Plasmid transfection is the first step in the generation of stably transformed animal cells and is also a useful tool for analyzing transient gene expression.
Michelle B.A. Keith, Patrick J. Farrell, Kostas Iatrou, Leo A. Behie   +3 more
doaj   +1 more source