Results 81 to 90 of about 280,113 (289)

Loss of IGF‐1R impairs DNA‐PKcs recruitment to chromatin leading to defective end‐joining

Molecular Oncology, EarlyView.
IGF‐1R promotes radioresistance by facilitating DNA‐PKcs recruitment to chromatin, enabling non‐homologous end‐joining (NHEJ) repair of double‐strand breaks. Inhibition or loss of IGF‐1R disrupts this recruitment to damage sites, driving compensatory reliance on microhomology‐mediated end‐joining (MMEJ) repair.
Matthew O. Ellis, Jack V. Mills, Wojciech Niedzwiedz, Valentine M. Macaulay   +3 more
wiley   +1 more source

Preparation, characterization, and transfection efficiency of low molecular weight polyethylenimine-based nanoparticles for delivery of the plasmid encoding CD200 gene [PDF]

, 2017
Fatemeh Nouri,1 Hossein Sadeghpour,2 Reza Heidari,3 Ali Dehshahri,1,4,5 1Department of Pharmaceutical Biotechnology, 2Department of Medicinal Chemistry, 3Department of Pharmacology and Toxicology, School of Pharmacy, 4Center for Nanotechnology in Drug ...
Dehshahri A, Nouri F, Sadeghpour H, Heidari R   +3 more
core  

Characterization of Ku70(2)-NLS as bipartite nuclear localization sequence for non-viral gene delivery. [PDF]

, 2012
Several barriers have to be overcome in order to achieve gene expression in target cells, e.g. cellular uptake, endosomal release and translocation to the nucleus.
Maucksch, Christof, Mittal, Rashmi, Rudolph, Carsten, Rashmi Mittal, Mittal Rashmi, Christof Maucksch, Alexander Bohla, Maucksch Christof, Carsten Rudolph, Joseph Rosenecker, Bohla, Alexander, Matschke, Johannes, Rosenecker Joseph, Bohla Alexander, Matschke Johannes, Rosenecker, Joseph, Rudolph Carsten, Johannes Matschke   +17 more
core   +1 more source

Primary Neuronal Culture and Transient Transfection

Bio-Protocol
Primary neuronal culture and transient transfection offer a pair of crucial tools for neuroscience research, providing a controlled environment to study the behavior, function, and interactions of neurons in vitro.
Shun- Tseng, Peng- Chen, Eric Hwang
doaj   +1 more source

USP29‐regulated noncanonical stabilization of the hypoxia‐inducible factor‐α in aggressive prostate cancer

Molecular Oncology, EarlyView.
We identify USP29 as the only DUB mirroring CA9 expression, a marker of hypoxia and HIF pathway activation associated with PCA aggressiveness. USP29 stabilizes HIF‐1α and HIF‐2α via a noncanonical mechanism that is independent of PHD/pVHL activity yet relies on proteasomal regulation, establishing USP29 as a previously unrecognized regulator of hypoxic
Amelie S Schober, Leire Moreno‐Cugnon, Laura Martínez‐Pérez, Amaia Ercilla, Amaia Zabala‐Letona, Adrián Vicente‐Barrueco, Maider Fagoaga‐Eugui, Saioa Garcia‐Longarte, Blanca Calle‐Ciborro, Encarnación Pérez‐Andrés, Onintza Carlevaris, Sara Pozo, Isabel Mendizabal, Ugo Mayor, Arkaitz Carracedo, Violaine Sée, Edurne Berra   +16 more
wiley   +1 more source

A Mechanistic Dissection of Polyethylenimine Mediated Transfection of Chinese Hamster Ovary Cells [PDF]

, 2013
Biopharmaceutical production through transient gene expression (TGE) is used within industry for the rapid supply of product for early stage testing.
Mozley, O L
core  

Finding novel vulnerabilities of hypomorphic BRCA1 alleles

Molecular Oncology, EarlyView.
Synthetic lethality screens performed to identify novel vulnerabilities often model complete gene loss, thereby overlooking patient‐derived hypomorphic mutations. In this study, we have performed genome‐wide CRISPR screens on BRCA1 hypomorphic mutations, showing BRCA1I26A behaves like wild‐type, while BRCA1R1699Q mimics deficiency. Furthermore, we have
Anne Schreuder, Klaas de Lint, Hồ Mỹ Phúc Võ, Mariana M. Góis, Rosalie A. Kampen, Marta San Martin Alonso, Ilse Nootenboom, Veronica Garzero, Tiemen J. Wendel, Rob M. F. Wolthuis, Sylvie M. Noordermeer   +10 more
wiley   +1 more source

Highly efficient transfection of human induced pluripotent stem cells using magnetic nanoparticles [PDF]

, 2018
Megan A Yamoah,1 Maryam Moshref,1 Janhavi Sharma,1 Wei Chun Chen,1 Hannah A Ledford,1 Jeong Han Lee,2 Karen S Chavez,1 Wenying Wang,2 Javier E López,1 Deborah K Lieu,1 Padmini Sirish,1,3 Xiao-Dong Zhang1,3 1Department of Internal Medicine ...
Lieu DK, López JE, Chen WC, Zhang XD, Yamoah MA, Sirish P, Wang W, Moshref M, Chavez KS, Sharma J, Ledford HA, Lee JH   +11 more
core  

A new role for clathrin adaptor proteins 1 and 3 in lipoplex trafficking [PDF]

, 2014
Intracellular protein trafficking through secretory and endocytic pathways depends on the function of adaptor proteins that bind motifs on cargo proteins.
Gumbs, Jade, Emma C Anderson, Alford, Justine E., Jade Gumbs, Anderson, Emma C., Emma C. Anderson, Justine E Alford, Justine E. Alford   +7 more
core   +1 more source

Oncogenic DMTF1β promotes cancer cell motility by regulating autophagy through ULK1 stabilization

Molecular Oncology, EarlyView.
In the current study, we demonstrate that the oncogene DMTF1β regulates ULK1 stability by reducing its proteasomal degradation in cancer cells. This stabilization enables ULK1 to induce autophagy, which in turn facilitates cancer cell migration. Consequently, reduced DMTF1β levels lead to decreased autophagy and impaired cancer cell migration.
Jun Xu, Nicolas J. Niklaus, Anna M. Schläfli, Igor Tokarchuk, Magali Humbert, Federico La Manna, Bich Vu, David Maul, Ramin Radpour, Marianna Kruithof‐de Julio, Inti Zlobec, Jörn Dengjel, Bruce E. Torbett, Mario P. Tschan   +13 more
wiley   +1 more source