Results 101 to 110 of about 280,113 (289)
Use of Flow Cytometry to Rapidly Optimize the Transfection of Animal Cells
BioTechniques, 2000 Plasmid transfection is the first step in the generation of stably transformed animal cells and is also a useful tool for analyzing transient gene expression.
Michelle B.A. Keith +3 more
doaj +1 more source
MiR‐513a promotes human erythroid differentiation by modulating c‐Jun
FEBS Open Bio, EarlyView.During early human erythropoiesis, miR‐513a promoted erythroid differentiation in primary human CD34+ hematopoietic stem‐progenitor cells and human TF‐1 erythroleukemic cells by indirectly decreasing c‐Jun and phospho‐c‐Jun expression, which are associated with increased GATA1 expression.
MinJung Kim +11 more
wiley +1 more source
FEBS Open Bio, EarlyView.Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane +11 more
wiley +1 more source
Novel biodegradable gene carriers based on oligomerized polyamines [PDF]
, 2006 Gene therapy is a very promising approach to treat or to prevent diseases. However, progress in this field is hindered by lack of suitable vectors. Current research focuses on the development of novel nonviral biodegradable gene carriers with improved ...
Klöckner, Julia
core
Evaluation of Transfected HEP-2 Cell Line Using ß-Galactosidase Reporter Assay System
مجلة بغداد للعلوم, 2011 Liposome-mediated transfection of cancer cells provide a valuable experimental technique to study cellular gene expression and may also be adapted for gene therapy studies.
Baghdad Science Journal
doaj +1 more source
YIPFα1A expression is regulated by multilayered molecular mechanisms
FEBS Open Bio, EarlyView.YIPFα1A, a five‐pass Golgi protein, is regulated at multiple layers. (1) Rare‐codon enrichment drives translation‐coupled mRNA decay. (2) A proximal 3′‐UTR element stabilizes mRNA. (3) A distal 3′‐UTR element included by alternate poly(A) site usage represses translation, which can be overridden by the proximal 3′‐UTR element.
Tokio Takaji +2 more
wiley +1 more source
In vitro transfection efficiency of CP/DNA nanoparticles. [PDF]
, 2014 (a) In vitro transfection efficiency of CP/DNA nanoparticles in both chondrocytes and synoviocytes compared to that of naked pDNA, CS/DNA nanoparticles, PEI (25 kDa)/DNA nanoparticles, and Lipofectamine™ 2000 (n = 3; 48 h post-transfection; error bars ...
Huading Lu (5520116) +3 more
core +1 more source
The spatial orientation of histidine via five-armed alkylamino siloxane improved the properties of the cationic gene delivery vector [PDF]
Biofunctional MaterialsThe efficiency of gene transfection using cationic polymers primarily depends on factors such as compact polyplex formation, cellular uptake, endosomal escape, cytoplasmic transport, nuclear entry, and the dissociation and release of plasmid from the ...
Viola B. Morris, Chandra P. Sharma
doaj +1 more source
High Transfection Efficiency of Neural Stem Cells with Magnetofection
BioTechniques, 2011 Primary neural stem cells (NSCs) can be cultivated and differentiated in vitro but are difficult to transfect using conventional methods. We describe a simple and rapid magnetofection-based method suitable for the lab bench as well as for high-throughput projects.
Cédric Sapet +6 more
openaire +3 more sources
FEBS Open Bio, EarlyView.The MRP4 transporter exports several drugs and signaling molecules. Here, we identified key promoter elements regulating basal MRP4 expression. Using reporter assays, we defined a conserved region with essential Sp1 and contributory Ets sites, which controlled basal MRP4 expression.
Debora Singer +7 more
wiley +1 more source