Results 121 to 130 of about 280,113 (289)

pH dependent high transfection efficiency of mouse neuroblastomas using TransFectin [PDF]

, 2006
Mouse neuroblastoma cell lines are often used in lieu of mouse primary neurons in ex vivo experiments, as they provide an easier platform for transfection, compared to the latter.
Chong, KW (15527354), Lee, AY (15527201), Koay, ES (15527207), Seet, SJ (15527312), Cheung, NS (15524789)   +4 more
core   +2 more sources

Colorectal cancer combination therapy using drug and gene co-delivered, targeted poly(ethylene glycol)-ε-poly(caprolactone) nanocarriers

Drug Design, Development and Therapy, 2018
Zhiyu Wang, Yaning Wei, Guotao Fang, Dan Hong, Lin An, Ting Jiao, Yan Shi, Aimin Zang Hebei Key Laboratory of Cancer Radiotherapy and Chemotherapy, Department of Medical Oncology, Affiliated Hospital of Hebei University, Baoding 071000, People’s ...
Wang Z, Wei Y, Fang G, Hong D, An L, Jiao T, Shi Y, Zang A   +7 more
doaj  

Spatial and Temporal Control of Cavitation Allows High In Vitro Transfection Efficiency in the Absence of Transfection Reagents or Contrast Agents.

PLoS ONE, 2015
Sonoporation using low-frequency high-pressure ultrasound (US) is a non-viral approach for in vitro and in vivo gene delivery. In this study, we developed a new sonoporation device designed for spatial and temporal control of ultrasound cavitation.
Kamel Chettab, Stéphanie Roux, Doriane Mathé, Emeline Cros-Perrial, Maxime Lafond, Cyril Lafon, Charles Dumontet, Jean-Louis Mestas   +7 more
doaj   +1 more source

Dose‐dependent hepatotoxicity of hydrogen peroxide in HepG2 cells and its modulation by CYP450 induction

FEBS Open Bio, EarlyView.
NMR metabolomics revealed concentration‐dependent metabolic perturbations in HepG2 cells exposed to H2O2. Rifampicin pretreatment enhanced metabolic competence, attenuated toxin‐induced alterations and produced metabolite profiles more consistent with human liver physiology, supporting the use of CYP450‐induced HepG2 models for improved in vitro ...
Maren Jinks, Garth L. Maker, Emily C. Davies, Berin A. Boughton, Samantha Lodge   +4 more
wiley   +1 more source

Protamine Enhances the Efficiency of Liposome-Mediated Gene Transfer in a Cultures Human Hepatoma Cell Line [PDF]

, 2008
Protamine, used clinically as an antidote for heparin, is a small protein with high arginine content and is potent in folding DNA. Protamine and DNA can form a compact structure, protecting DNA from digestion by intracellular enzymes.
NI, YEN-HSUAN;HSU, HONG-YUAN;CHEN, PEI-JER;CHANG, MEI-HWEI, 倪衍玄;許宏遠;陳培哲;張美惠   +1 more
core  

DNA−Poly(diallyldimethylammonium chloride) Complexation and Transfection Efficiency

The Journal of Physical Chemistry B, 2010
The present work assesses the influence of the cationic charge density (CD) and the cationic valence of poly(diallyldimethylammonium chloride) (pDADMAC) on the DNA compaction and subsequent transfection. Four homopolymers (CD = 1, with different valences) and one copolymer, poly(acrylamide-co-diallyldimethylammonium chloride) (coDADMAC) (CD < 1 ...
Alatorre-Meda, Manuel, Taboada, Pablo, Krajewska, Barbara, Willemeit, Markus, Deml, Alexander, Klösel, Roland, Rodríguez, Julio R.   +6 more
openaire   +3 more sources

Delivery of DNA‐encoded vaccines and proteins in mice using cutaneous suction‐mediated transfection

FEBS Open Bio, EarlyView.
Cutaneous suction‐mediated transfection in mice for delivery of DNA‐encoded vaccines and proteins. A critical limitation of DNA vaccines and other therapeutics is transfection in vivo to produce the encoded antigens or therapeutic proteins. Cutaneous suction‐based methods have demonstrated effectiveness in many animal models and have been successfully ...
Emran O. Lallow, Isabel Brandtjen, Yaxin Mo, Melissa Gulley, Louis Osorio, Sagar Kudchodkar, Nandita C. Jhumur, Christine C. Roberts, Lisa K. Denzin, David I. Shreiber, Biju Parekkadan, Hao Lin, Joel N. Maslow   +12 more
wiley   +1 more source

Molecular Strings Significantly Improved the Gene Transfection Efficiency of Polycations [PDF]

, 2018
High transfection efficiency and low cytotoxicity are the two key factors to be considered in the design of gene carriers. Herein, a novel and versatile gene carrier (PLL-RT) was prepared by introducing “molecular string” RT (i.e., p-toluylsulfonyl ...
Huapan Fang, Lin Lin, Xuesi Chen, Jie Chen, Zhaopei Guo (2419204), Caina Xu, Zhaopei Guo, Huayu Tian (1715563), Jiayan Wu (204411), Caina Xu (5622803), Huayu Tian, Jie Chen (5892), Xuesi Chen (539648), Pingjie Sun, Jiayan Wu, Lin Lin (46073), Huapan Fang (5741486), Pingjie Sun (3344327)   +17 more
core   +1 more source

Identification of (20R)‐protopanaxadiol from Panax ginseng as a novel anti‐SARS‐CoV‐2 compound

FEBS Open Bio, EarlyView.
We established a noninfectious BAC‐based SARS‐CoV‐2 replicon that enables antiviral screening under BSL‐2 conditions. Using this platform, we screened 373 food‐derived compounds and identified (20R)‐protopanaxadiol from Panax ginseng as a novel inhibitor of SARS‐CoV‐2 RNA replication, highlighting the value of safe replicon systems for antiviral ...
Midori Takeda, Natsue Maezono, Rina Uchikoshi, Nobuyuki Kato, Masanori Ikeda   +4 more
wiley   +1 more source

Developmental and Epileptic Encephalopathy due to Biallelic Pathogenic Variants in PIGM

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective PIGM encodes a critical enzyme in the glycosylphosphatidylinositol (GPI)‐anchor biosynthesis pathway. While promoter‐region mutations in PIGM have been associated with a relatively mild phenotype characterized by portal vein thrombosis and absence seizures, recent evidence suggests that coding‐region mutations result in a more severe
Júlia Sala‐Coromina, Anna Marcé‐Grau, Barbara Masotto, Marta Codina, Lamia BenJemaa, Yasmina Elaribi, Mónica Martinez‐Gallo, Roger Colobran, Angel Sanchez‐Montañez, Irene Valenzuela, Yoshiko Murakami, Alfons Macaya   +11 more
wiley   +1 more source