Results 41 to 50 of about 280,113 (289)
Enhancement of chitosan nanoparticle-facilitated gene transfection by ultrasound both in vitro and in vivo [PDF]
, 2017 In recent years, inefficiency of transfection and the lack of safe gene vectors have limited the feasibility of gene therapy. Fabrication of a vector that is safe and has high transfection efficiency is crucial for the development of successful gene ...
Yang, Shu-Jyuan;Chang, Szu-Min;Tsai, Kun-Che;Tsai, Han-Min;Chen, Wen-Shiang;Shieh, Ming-Jium +1 more
core +1 more source
Supplementary Table 1 - High transfection efficiency and cell viability of immune cells with nanomaterials-based transfection reagent [PDF]
, 2022 High transfection efficiency and cell viability of immune cells with nanomaterials-based transfection reagent - Supplementary Table 1 Transfection efficiencies on day
Song Wang (144220), Dan Tian (697937)
core +1 more source
BMC Biotechnology, 2002 Background Cationic lipid DNA complexes based on DOTAP (1,2-dioleoyl-3-(trimethyammonium) propane) and mixtures of DOTAP and cholesterol (DC) have been previously optimized for transfection efficiency in the absence of serum and used as a non-viral gene ...
Überla Klaus +2 more
doaj +1 more source
Cells, 2023 Transient transfection of foreign DNA is the most widely used laboratory technique to study gene function and product. However, the transfection efficiency depends on many parameters, including DNA quantity and quality, transfection methods and target ...
Théo Juncker +2 more
doaj +1 more source
Gene transfection efficiency. [PDF]
, 2023 (A) quantification of GFP- cellular uptake by flow cytometry analysis after transfection, (B) Cell viability after transfection in HeLa cells.
Elcey C. Daniel (13259405) +7 more
core +1 more source
Journal of Nanobiotechnology, 2011 Background Reproducibly high transfection rates with low methodology-induced cytotoxic side effects are essential to attain the required effect on targeted cells when exogenous DNA is transfected. Different approaches and modifications such as the use of
Nolte Ingo +9 more
doaj +1 more source
Estimating transfection efficiency in differentiated and undifferentiated neural cells. [PDF]
BMC Res Notes, 2019 Objective Delivery of constructs for silencing or over-expressing genes or their modified versions is a crucial step for studying neuronal cell biology.
Alabdullah AA +9 more
europepmc +2 more sources
An isoform of 14‐3‐3 protein regulates transbilayer lipid movement at the plasma membrane
FEBS Letters, EarlyView.Loss of 14‐3‐3ζ in CHO cells confers resistance to exogenous phosphatidylserine (PS) and impairs endocytosis‐independent inward flip‐flop of fluorescent PS at the plasma membrane. RNAi‐mediated knockdown reproduces this defect, while no additive effect is seen in ATP11C‐deficient cells.
Akiko Yamaji‐Hasegawa +3 more
wiley +1 more source
Optical sorting and photo-transfection of mammalian cells [PDF]
, 2010 Recently, laser light sources of different regimes have emerged as an essential tool in the biophotonics research area. Classic applications include, for example: manipulating single cells and their subcellular organelles, sorting cells in microfluidic ...
Mthunzi, Patience
core
Transfection efficiency and immortalization efficiency after transfection with HPV16, HPV18 or SV40 DNA. [PDF]
, 2018 A. Transfection efficiency of beta-galactosidase reporter gene in early passage cells from ectocervix, TZ and endocervix. The bars represent the mean ± standard error of 13 experiments using samples from different donors. B. Immortalization efficiency of
Craig D. Woodworth (711669) +4 more
core +1 more source