Results 41 to 50 of about 3,929,037 (185)
Journal of Nanobiotechnology, 2011 Background Reproducibly high transfection rates with low methodology-induced cytotoxic side effects are essential to attain the required effect on targeted cells when exogenous DNA is transfected. Different approaches and modifications such as the use of
Nolte Ingo +9 more
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High-efficiency formation of influenza virus transfectants [PDF]
Journal of Virology, 1991 cDNA-derived RNAs were introduced into the genomes of influenza viruses by using an improved ribonucleoprotein (RNP) transfection protocol. Up to 10(5) viral transfectants with a novel neuraminidase gene could be obtained by using a 35-mm dish (10(6) cells) for RNP transfection.
M, Enami, P, Palese
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BMC Biotechnology, 2009 Background Transfection in mammalian cells based on liposome presents great challenge for biological professionals. To protect themselves from exogenous insults, mammalian cells tend to manifest poor transfection efficiency.
Pan Jin-Shui +9 more
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Molecules, 2016 To solve the problem of transfection efficiency vs. cytotoxicity and tumor-targeting ability when polyethylenimine (PEI) was used as a nonviral gene delivery vector, new degradable PEI polymers were synthesized via cross-linking low-molecular-weight PEI ...
Jing Hu +7 more
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, 2022 Supplementary figure 1 - High transfection efficiency and cell viability of immune cells with nanomaterials-based transfection ...
Song Wang (144220), Dan Tian (697937)
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Applied Sciences, 2021 Long duration electric pulses are frequently used to facilitate DNA electrotransfer into cells and tissues, while electroporation pulses can be combined with electrophoresis to maximize the transfection efficiency.
Eivina Radzevičiūtė +6 more
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The effect of environmental pH on polymeric transfection efficiency [PDF]
Biomaterials, 2012 Although polymers, polyplexes, and cells are exposed to various extracellular and intracellular pH environments during polyplex preparation and polymeric transfection, the impact of environmental pH on polymeric transfection has not yet been investigated.
Han Chang, Kang +3 more
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Structural aspects of histone H1-DNA complexes and their relation to transfection efficiency
, 2022 S.107-117During transfection, polycation-DNA complexes are normally diluted by the transfection medium, which often contains salt in the physiological concentration range and serum. It is not exactly known to what extent this dilution step influences the
Heuer, D. +6 more
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Optimization of chemical transfection in airway epithelial cell lines
BMC BiotechnologyBackground Chemical transfection is a widely employed technique in airway epithelium research, enabling the study of gene expression changes and effects. Additionally, it has been explored for its potential application in delivering gene therapies. Here,
Tony J. F. Guo +5 more
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Transfection efficiency of rE.mi of different passages.
, 2014 Transfection efficiency of rE.mi of different passages.
Xun Suo (317782) +5 more
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