Results 21 to 30 of about 270,633 (290)

A call for caution in analysing mammalian co-transfection experiments and implications of resource competition in data misinterpretation

Nature Communications, 2021
Transient transfections are routinely used in basic and synthetic biology studies to unravel pathway regulation and to probe and characterise circuit designs.
Roberto Di Blasi, Masue M. Marbiah, Velia Siciliano, Karen Polizzi, Francesca Ceroni   +4 more
doaj   +1 more source

MicroRNA-Mediated Direct Reprogramming of Human Adult Fibroblasts Toward Cardiac Phenotype

Frontiers in Bioengineering and Biotechnology, 2020
Modulation of microRNA expression holds the promise to achieve direct reprogramming of fibroblasts into cardiomyocyte-like cells as a new strategy for myocardial regeneration after ischemic heart disease.
C. Paoletti, C. Divieto, G. Tarricone, F. Di Meglio, D. Nurzynska, V. Chiono   +5 more
doaj   +1 more source

Rapid Lentiviral Vector Producer Cell Line Generation Using a Single DNA Construct

Molecular Therapy: Methods & Clinical Development, 2020
Stable suspension producer cell lines for the production of vesicular stomatitis virus envelope glycoprotein (VSVg)-pseudotyped lentiviral vectors represent an attractive alternative to current widely used production methods based on transient ...
Yu Hua Chen, Celeste Pallant, Christopher J. Sampson, Alessia Boiti, Sabine Johnson, Pijus Brazauskas, Philip Hardwicke, Michela Marongiu, Vanesa M. Marinova, Marlene Carmo, Nathan P. Sweeney, Ashkenaz Richard, Anthony Shillings, Peter Archibald, Eva Puschmann, Bernadette Mouzon, David Grose, Miriam Mendez-Tavio, Mao Xiang Chen, Stephen R.C. Warr, Tarik Senussi, Paul S. Carter, Sean Baker, Cindy Jung, Martijn H. Brugman, Steven J. Howe, Conrad A. Vink   +26 more
doaj   +1 more source

Ezh2-dCas9 and KRAB-dCas9 enable engineering of epigenetic memory in a context-dependent manner. [PDF]

, 2019
BackgroundRewriting of the epigenome has risen as a promising alternative to gene editing for precision medicine. In nature, epigenetic silencing can result in complete attenuation of target gene expression over multiple mitotic divisions.
Bates, Sofie L, Carter, Sakereh S, Farnham, Peggy J, Fink, Kyle D, Halmai, Julian, Nisson, Karly A, O'Geen, Henriette, Rhie, Suhn K, Segal, David J   +8 more
core   +2 more sources

Demethylation using the epigenetic modifier, 5-azacytidine, increases the efficiency of transient transfection of macrophages

Journal of Lipid Research, 2005
This study was aimed at developing a method for high-efficiency transient transfection of macrophages. Seven methods were evaluated for transient transfection of murine macrophage RAW 264.7 cells.
Geneviève Escher, Anh Hoang, Suzan Georges, Urbain Tchoua, Assam El-Osta, Zygmunt Krozowski, Dmitri Sviridov   +6 more
doaj   +1 more source

A novel dual-plasmid platform provides scalable transfection yielding improved productivity and packaging across multiple AAV serotypes and genomes

Molecular Therapy: Methods & Clinical Development, 2023
Transient transfection of mammalian cells using plasmid DNA is a standard method to produce adeno-associated virus (AAV) vectors allowing for flexible and scalable manufacture.
Laura P. van Lieshout, Miranda Rubin, Katrina Costa-Grant, Stacy Ota, Diane Golebiowski, Troy Panico, Eli Wiberg, Klaudia Szymczak, Richard Gilmore, Marissa Stanvick, Brenda Burnham, Jeff Gagnon, Ifeyinwa Iwuchukwu, Guang Yang, Iraj Ghazi, Alex Meola, Ryan Dickerson, Thomas Thiers, Luke Mustich, April Hayes, Israel Rivas, Jason Lotterhand, Nancy Avila, James McGivney, Jin Yin, Tim Kelly   +25 more
doaj   +1 more source

Mutations in human dynamin block an intermediate stage in coated vesicle formation [PDF]

, 1993
The role of human dynamin in receptor-mediated endocytosis was investigated by transient expression of GTP-binding domain mutants in mammalian cells. Using assays which detect intermediates in coated vesicle formation, the dynamin mutants were found to ...
Damke, Hanna, Meyerowitz, Elliot M., Redelmeier, Thomas E., Schmid, Sandra L., Tisdale, Ellen J., van der Bliek, Alexander M.   +5 more
core   +3 more sources

Efficient isolation of protoplasts from rice calli with pause points and its application in transient gene expression and genome editing assays

Plant Methods, 2020
Background An efficient in vivo transient transfection system using protoplasts is an important tool to study gene expression, metabolic pathways, and multiple mutagenesis parameters in plants.
Snigdha Poddar, Jaclyn Tanaka, Jamie H. D. Cate, Brian Staskawicz, Myeong-Je Cho   +4 more
doaj   +1 more source

Ebola virus VP35 induces high-level production of recombinant TPL-2–ABIN-2–NF-κB1 p105 complex in co-transfected HEK-293 cells [PDF]

, 2013
Activation of PKR (double-stranded-RNA-dependent protein kinase) by DNA plasmids decreases translation, and limits the amount of recombinant protein produced by transiently transfected HEK (human embryonic kidney)-293 cells.
Apostolaki, Baldi, Beinke, Beinke, Beinke, Beinke, Beinke, Belich, Bouwmeester, Ciechanover, Cohen, Coope, Costa, Dauber, Dumitru, Durocher, Elke MÜHLBERGER, Feng, Gantke, Geisse, George, Gray, Jia, Jia, Julia Janzen, Kabouridis, Kaufman, Lang, Liu, Nicholas A. Morrice, Papoutsopoulou, Pomerantz, Robinson, Roget, Sabrina Boussouf, Salmeron, Salmeron, Savinova, Schlaeger, Schumann, Steven Howell, Steven C. Ley, Thorsten Gantke, Waterfield, Waterfield, Wu, Yang, Zhao   +47 more
core   +1 more source

A transient CRISPR/Cas9 expression system for genome editing in Trypanosoma brucei

BMC Research Notes, 2020
Objective Generation of knockouts and in situ tagging of genes in Trypanosoma brucei has been greatly facilitated by using CRISPR/Cas9 as a genome editing tool.
Sebastian Shaw, Sebastian Knüsel, Sarah Hoenner, Isabel Roditi   +3 more
doaj   +1 more source