Results 11 to 20 of about 270,633 (290)

Transient transfection using 222 nm far UV-C irradiation [PDF]

Scientific Reports
Ultraviolet (UV) light is never used for gene transfer because it damages DNA and harms cellular and plasmid DNA. A light source selectively radiating Far UV-C (F-UV) light causes less DNA damage.
Mane Nishimura, Yuki Shimizu, Tomohiro Fujii, Yu Okada, Takeshi Yamamoto, Yoshimasa Ogawa, Toru Koi, Yutaka Suehiro, Takahiro Yamasaki, Jun Nishikawa   +9 more
doaj   +2 more sources

An effective method to enhance transient gene expression by transfecting high-density CHO cells [PDF]

AMB Express
Chinese hamster ovary (CHO) cells are the predominant host for recombinant protein production. While transient gene expression (TGE) systems provide a faster, more cost-effective alternative to stable gene expression (SGE), their application is often ...
Yu-shun Fu, Xue-lian Han, Wei-dong Li, Lin-lin Shan, Shao-lei Geng, Chun-liu Mi, Wei-hua Dong, Tian-yun Wang, Xiao-yin Wang   +8 more
doaj   +2 more sources

Rapid expression of therapeutic antibodies in mammalian cells via mRNA transfection [PDF]

mAbs
Messenger RNA (mRNA) has emerged as a powerful tool for protein expression in clinical settings, yet its potential as a platform for biologics manufacturing remains underexplored.
Thornwit Chavalparit, Craig Barry, Helen Gunter, Marianne Gillard, Timothy Mercer, Esteban Marcellin   +5 more
doaj   +2 more sources

Optimizing conditions for calcium phosphate mediated transient transfection. [PDF]

Saudi J Biol Sci, 2017
Calcium phosphate mediated transfection has been used for delivering DNA into mammalian cells in excess of 30 years due to its most low cost for introducing recombinant DNA into culture cells. However, multiple factors affecting the transfect efficiency are commonly recognized meanwhile for years, the low transfection efficiency of this approach on ...
Guo L, Wang L, Yang R, Feng R, Li Z, Zhou X, Dong Z, Ghartey-Kwansah G, Xu M, Nishi M, Zhang Q, Isaacs W, Ma J, Xu X.   +13 more
europepmc   +3 more sources

Secondary Transient Transfection of Stably Transfected Endothelial Cells [PDF]

BioTechniques, 2000
Reza Forough, James R. Hawker
doaj   +3 more sources

Co-Transfected Plasmids Enhance Transient Expression of Reporter Genes [PDF]

BioTech
Transient DNA transfection is routinely used to study gene function and elucidate the regulation of biological pathways, and it is also widely applied in biotechnology for large-scale recombinant protein production.
Shih-Yen Lo, Chee-Hing Yang, Yu-Ru Chan, Yi-Tzu Chao, Meng-Jiun Lai, Hui-Chun Li   +5 more
doaj   +2 more sources

Predicting Transiently Expressed Protein Yields: Comparison of Transfection Methods in CHO and HEK293

Pharmaceutics, 2022
Therapeutic proteins are currently at the apex of innovation in pharmaceutical medicine. However, their industrial production is technically challenging and improved methods for transient transfection of mammalian cell cultures are necessary. We aimed to
Ly Porosk, Jekaterina Nebogatova, Heleri Heike Härk, Birgit Vunk, Piret Arukuusk, Urve Toots, Mart Ustav, Ülo Langel, Kaido Kurrikoff   +8 more
doaj   +1 more source

100-liter transient transfection [PDF]

Cytotechnology, 2002
This is the first report of two successful 100 l scale transienttransfections in a standard stirred bioreactor. More than half a gram of a monoclonal antibody (IgG) were produced in less than 10 days using a technology called large-scale transient gene expression(LS-TGE). Suspension adapted HEK 293 EBNA SF cells were transfectedwithin a 150 l (nominal)
Girard P, Derouazi M, Baumgartner G, Bourgeois M, Jordan M, Jacko B, Wurm FM   +6 more
openaire   +3 more sources

Targeted knock-down of miR21 primary transcripts using snoMEN vectors induces apoptosis in human cancer cell lines [PDF]

, 2015
We have previously reported an antisense technology, 'snoMEN vectors', for targeted knock-down of protein coding mRNAs using human snoRNAs manipulated to contain short regions of sequence complementarity with the mRNA target. Here we characterise the use
A Kozomara, A Rodriguez, AG Matera, AK Leung, AM Burroughs, AM Cheng, Angus I. Lamond, B Bai, C Ender, Christophe Antoniewski, CL Ahlenstiel, D De Paula, D Iliopoulos, Dalila Bensaddek, EC Lai, EC Lai, EJ Chapman, EJ Jung, Fabio Avolio, FJ Sheedy, FM Boisvert, FM Boisvert, G Reimer, GS Pall, I Rebbapragada, J Elmen, J Krutzfeldt, J Liu, J Weischenfeldt, JC Politz, JS Andersen, JS Andersen, K Yamada, Kayo Yamada, KK Farh, L Agranat, L Trinkle-Mulcahy, LB Frankel, LB Weinstein, M Negrini, M Ono, M Ono, M Ono, M Scherr, ME Hatley, Motoharu Ono, MS Scott, MS Scott, N Bushati, O Isken, RS Coffin, S Baskerville, S Griffiths-Jones, SD Selcuklu, T Kiss, Vackar Afzal, Y Ahmad, YK Kim, YS Lee, YW Lam   +59 more
core   +13 more sources

Magnetic Selection of Transiently Transfected Cells [PDF]

BioTechniques, 1996
An efficient and rapid method for selecting transiently transfected cells is described. A plasmid encoding for a neural cell-specific surface marker is co-transfected into mammalian cells along with the gene of interest. After uptake and expression of these two plasmids, the transfected cells are immuno-adsorbed to magnetic beads pre-coated with ...
Schneider S, Rusconi S
openaire   +4 more sources