Eimeria tenella protein trafficking: differential regulation of secretion versus surface tethering during the life cycle [PDF]
, 2017 Eimeria spp. are intracellular parasites that have a major impact on poultry. Effective live vaccines are available and the development of reverse genetic technologies has raised the prospect of using Eimeria spp.
Blake, D P +4 more
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Serum-free transfection of CHO cells with chemically defined transfection systems and investigation of their potential for transient and stable transfection [PDF]
Cytotechnology, 2009 The generation of transgenic cell lines is acquired by facilitating the uptake and integration of DNA. Unfortunately, most of the systems generating stable expression systems are cost and time-consuming and transient expression is optimized to generate milligram amounts of the recombinant protein.
Hannes, Reisinger +3 more
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Horticultural Plant JournalProtoplast-based transient gene expression system has been widely used in plant genome editing because of its simple operation and less time-consuming.
Xiaoxiao Yu +8 more
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Animals, 2023 The solute transport protein family 11 A1 (SLC11A1), also recognized as natural resistance-associated macrophage protein 1 (NRAMP1), represents a transmembrane protein encoded by the SLC11A1 gene.
Yuqing Chong +6 more
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Specific binding of the methyl binding domain protein 2 at the BRCA1-NBR2 locus [PDF]
, 1996 The methyl-CpG binding domain (MBD) proteins are key molecules in the interpretation of DNA methylation signals leading to gene silencing. We investigated their binding specificity at the constitutively methylated region of a CpG island containing the ...
Auriol, Emilie +3 more
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Non-GMP plasmid production for transient transfection in bioreactors [PDF]
Cytotechnology, 2001 We describe a generic plasmid purification process for producing DNA for larger-scale transient transfection. Data on plasmid quality with regard to residual protein, endotoxin content and presence of different plasmid forms is given. The effects of contaminants and plasmid forms on expression levels of TNFRp55 and SEAP are discussed.
G, Schmid, E J, Schlaeger, B, Wipf
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Loss of signal transducer and activator of transcription 1 is associated with prostate cancer recurrence [PDF]
, 2015 STAT1 loss has previously been implicated in cell line studies to modify prostate cancer cell growth and survival, however the clinical significance of this has not previously been established.
Edwards, Joanne +6 more
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Transient Mammalian Cell Transfection with Polyethylenimine (PEI) [PDF]
, 2013 Standard protein expression systems, such as E. coli, often fail to produce folded, monodisperse, or functional eukaryotic proteins (see Small-scale Expression of Proteins in E. coli). The expression of these proteins is greatly benefited by using a eukaryotic system, such as mammalian cells, that contains the appropriate folding and posttranslational ...
Longo, Patti A. +3 more
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Targeting mitochondrial 18 kDa translocator protein (TSPO) regulates macrophage cholesterol efflux and lipid phenotype [PDF]
, 2014 The aim of the present study was to establish mitochondrial cholesterol trafficking 18 kDa translocator protein (TSPO) as a potential therapeutic target, capable of increasing macrophage cholesterol efflux to (apo)lipoprotein acceptors.
Anne-Marie Allen +46 more
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Subcellular localization of the five members of the human steroid 5α-reductase family [PDF]
, 2017 In humans the steroid 5a-reductase (SRD5A) family comprises five integral membrane enzymes that carry out reduction of a double bond in lipidic substrates: D4-3-keto steroids, polyprenol and trans-enoyl CoA.
Asteriti, ITALIA ANNA +10 more
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