Results 51 to 60 of about 5,757,325 (187)

Suitability of a CMV/EGFP cassette to monitor stable expression from human artificial chromosomes but not transient transfer in the cells forming viable clones [PDF]

, 2004
Human artificial chromosomes (HACs) were generated by transfer of telomerized PAC constructs containing alpha satellite DNA of various human chromosomes. To monitor which cells took up constructs and subsequently formed stable clones under blasticidin S (
Laner, A., Schindelhauer, D., Christan, S., Schwarz, T., Laner, A.;Schwarz, T.;Christan, S.;Schindelhauer, D.   +4 more
core   +1 more source

CRISPR/Cas9 mediated editing of the Quorn fungus Fusarium venenatum A3/5 by transient expression of Cas9 and sgRNAs targeting endogenous marker gene PKS12

Fungal Biology and Biotechnology, 2021
Background Gene editing using CRISPR/Cas9 is a widely used tool for precise gene modification, modulating gene expression and introducing novel proteins, and its use has been reported in various filamentous fungi including the genus Fusarium.
Fiona M. Wilson, Richard J. Harrison
doaj   +1 more source

Over-expression of a chimeric gene of the transcriptional co-activator MBF1 fused to the EAR repressor motif causes developmental alteration in Arabidopsis and tomato [PDF]

, 2008
Transcriptional co-activators of the Multiprotein Bridging Factor1 (MBF1) type belong to a multigenic family that encode key components of the machinery controlling gene expression by communicating between transcription factors and the basal ...
Regad, Farid, Latché, Alain, Hommel, Marie, Khalil Ahmad, Qaiser, Pech, Jean-Claude, Jaimes Miranda, Fabiola, Pouzet, Cécile, Mila, Isabelle, Bouzayen, Mondher   +8 more
core   +1 more source

Transient Expression Assay in NahG Arabidopsis Plants Using Agrobacterium tumefaciens

Bio-Protocol, 2018
Agrobacterium-mediated transient expression has greatly contributed to research in molecular plant biology but has low efficiency and inconsistency in Arabidopsis thaliana (Arabidopsis).
Pepe Cana-Quijada, Eduardo Bejarano, Rosa Lozano-Durán, Tabata Rosas-Díaz   +3 more
doaj   +1 more source

Methods for transient assay of gene function in floral tissues

Plant Methods, 2007
Background There is considerable interest in rapid assays or screening systems for assigning gene function. However, analysis of gene function in the flowers of some species is restricted due to the difficulty of producing stably transformed transgenic ...
Pathirana Nilangani N, Waugh Toni L, Hunter Donald A, Bennett Michael J, Schwinn Kathy E, Shang Yongjin, Brummell David A, Jameson Paula E, Davies Kevin M   +8 more
doaj   +1 more source

Suitability of transiently expressed antibodies for clinical studies: product quality consistency at different production scales

mAbs, 2022
Transgenic human monoclonal antibodies derived from humanized mice against different epitopes of the Middle East respiratory syndrome coronavirus (MERS-CoV), and chimeric llama-human bispecific heavy chain-only antibodies targeting the Rift Valley fever ...
Sara Rodriguez-Conde, Sophie Inman, Viv Lindo, Leanne Amery, Alison Tang, Uche Okorji-Obike, Wenjuan Du, Berend-Jan Bosch, Paul J. Wichgers Schreur, Jeroen Kortekaas, Isabel Sola, Luis Enjuanes, Laura Kerry, Katharina Mahal, Martyn Hulley, Olalekan Daramola   +15 more
doaj   +1 more source

Transient Gene Expression in Tobacco using Gibson Assembly and the Gene Gun [PDF]

Journal of Visualized Experiments, 2014
In order to target a single protein to multiple subcellular organelles, plants typically duplicate the relevant genes, and express each gene separately using complex regulatory strategies including differential promoters and/or signal sequences. Metabolic engineers and synthetic biologists interested in targeting enzymes to a particular organelle are ...
Matthew D, Mattozzi, Mathias J, Voges, Pamela A, Silver, Jeffrey C, Way   +3 more
openaire   +2 more sources

Cytomegalovirus replicon-based regulation of gene expression in vitro and in vivo [PDF]

, 2011
There is increasing evidence for a connection between DNA replication and the expression of adjacent genes. Therefore, this study addressed the question of whether a herpesvirus origin of replication can be used to activate or increase the expression of ...
Mohr, Hermine, Eckhard Wolf, Marlon R. Schneider (160130), Ulrich H Koszinowski, Laura Scrivano (160134), Eckhard Wolf (39870), Koszinowski Ulrich H., Ruzsics, Zsolt, Schnieke Angelika, Christian A. Mohr (160123), Ulrich H. Koszinowski (152143), Marlon R. Schneider, Mohr Hermine, Schneider Marlon R., Dahlhoff, Maik, Hermine Mohr (160119), Zsolt Ruzsics (75383), Simone Kraner-Schreiber, Schneider, Marlon R., Christian A Mohr, Laura Scrivano, Kraner-Schreiber, Simone, Maik Dahlhoff, Simone Kraner-Schreiber (160141), Ruzsics Zsolt, Maik Dahlhoff (160144), Adler Barbara, Koszinowski, Ulrich H., Kraner-Scheiber, Simone, Zsolt Ruzsics, Mohr, Christian A., Kraner-Schreiber Simone, Schnieke, Angelika, Adler, Barbara, Wolf Eckhard, Dahlhoff Maik, Barbara Adler, Ulrich H. Koszinowski, Christian A. Mohr, Mohr Christian A., Scrivano Laura, Wolf, Eckhard, Hermine Mohr, Marlon R Schneider, Scrivano, Laura, Angelika Schnieke (133290), Barbara Adler (160138), Angelika Schnieke   +47 more
core   +2 more sources

Transient, Inducible, Placenta-Specific Gene Expression in Mice

Endocrinology, 2012
AbstractMolecular understanding of placental functions and pregnancy disorders is limited by the absence of methods for placenta-specific gene manipulation. Although persistent placenta-specific gene expression has been achieved by lentivirus-based gene delivery methods, developmentally and physiologically important placental genes have highly stage ...
Xiujun, Fan, Matthew, Petitt, Matthew, Gamboa, Mei, Huang, Sabita, Dhal, Maurice L, Druzin, Joseph C, Wu, Yanru, Chen-Tsai, Nihar R, Nayak   +8 more
openaire   +3 more sources

Delivery of human apolipoprotein (apo) E to liver by an [E1(-), E3(-), polymerase(-), pTP(-)] adenovirus vector containing a liver-specific promoter inhibits atherogenesis in immunocompetent apoE-deficient mice [PDF]

, 2006
Recombinant adenovirus (rAd)-mediated apoE gene transfer to the liver of apoE(-/-) mice is anti-atherogenic. However, first generation rAd vectors were associated with immune clearance of transduced hepatocytes, while an improved [E1(-), E3(-) polymerase(
Amalfitano, A, Owen, JS, Dickson, G, Graham, IR, Harris, JD   +4 more
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