Results 71 to 80 of about 5,757,325 (187)

A new toolset for protein expression and subcellular localization studies in citrus and its application to citrus tristeza virus proteins

Plant Methods, 2018
Background Transient gene expression is a powerful tool to study gene function in plants. In citrus, Agrobacterium transformation is the method of choice for transient expression studies, but this method does not work efficiently with many gene ...
Amit Levy, Choaa El-Mochtar, Chunxia Wang, Michael Goodin, Vladimir Orbovic   +4 more
doaj   +1 more source

Transient gene expression in aleurone protoplasts isolated from developing caryopses of barley and wheat.

, 1989
Methods have been developed for the isolation of aleurone protoplasts from developing caryopses of Hordeum vulgare and Triticum aestivum in order to study transient expression of introduced genes.
Kreis, M., Topping, J., Lee, B., Jones, M.G.K., Murdoch, K., Jones, M. G. K.   +5 more
core  

Gene Expression Modifications by Temperature-Toxicants Interactions in Caenorhabditis elegans [PDF]

, 2011
Although organophosphorus pesticides (OP) share a common mode of action, there is increased awareness that they elicit a diverse range of gene expression responses.
Vinuela Rodriguez, A., Kammenga Jan E., Joost A. G. Riksen, Riksen Joost A. G., Ana Viñuela, L Basten Snoek, Viñuela Ana, Jan E. Kammenga, Joost A G Riksen, Kammenga, J.E., Snoek, L.B., L. Basten Snoek, Jan E Kammenga, Snoek L. Basten, Riksen, J.A.G.   +14 more
core   +1 more source

Transfection of microRNA mimics should be used with caution

Frontiers in Genetics, 2015
Transient transfection of chemically synthesized microRNA (miRNA) mimics is being used extensively to study the functions and mechanisms of endogenous miRNAs.
Hyun Yong eJin, Alicia eGonzalez-Martin, Ana eMiletic, Maoyi eLai, Sarah eKnight, Mohsen eSabouri-Ghomi, Steven R Head, Matthew S Macauley, Robert eRickert, Changchun eXiao   +9 more
doaj   +1 more source

Knowledge-based gene expression classification via matrix factorization [PDF]

, 2008
Motivation: Modern machine learning methods based on matrix decomposition techniques, like independent component analysis (ICA) or non-negative matrix factorization (NMF), provide new and efficient analysis tools which are currently explored to analyze ...
Schachtner, R., Lang, E. W., Tomé, A. M., Gómez Vilda, P., Vilda, P.G., Theis, Fabian J., Theis, F. J., Knollmüller, P., Schmitz, G., Lang, Elmar, Lang, E.W., Lutter, D., Stetter, M., Theis, F.J., Tomé, A.M.   +14 more
core   +2 more sources

Fuzzy Clustering Models for Gene Expression Data Analysis [PDF]

With the advent of microarray technology, it is possible to monitor gene expression of tens of thousands of genes in parallel. In order to gain useful biological knowledge, it is necessary to study the data and identify the underlying patterns, which ...
Wang, Yu
core  

CD69 is a TGF-β/1α,25-dihydroxyvitamin D3 target gene in monocytes [PDF]

, 2013
CD69 is a transmembrane lectin that can be expressed on most hematopoietic cells. In monocytes, it has been functionally linked to the 5-lipoxygenase pathway in which the leukotrienes, a class of highly potent inflammatory mediators, are produced ...
Thea K Wöbke, Thea K. Wöbke, Bernd L. Sorg, Dieter Steinhilber, Wöbke, Thea Katharina, Andreas von Knethen, Bernd L Sorg, Sorg, Bernd L., Knethen, Andreas von, Steinhilber, Dieter   +9 more
core   +1 more source

Intestinal gene expression in pigs: effects of reduced feed intake during weaning and potential impact of dietary components [PDF]

, 2011
The weaning transition is characterised by morphological, histological and microbial changes, often leading to weaning-associated disorders. These intestinal changes can partly be ascribed to the lack of luminal nutrition arising from the reduced feed ...
Bauer, E., Verstegen, M.W.A., Metzler-Zebeli, B.U., Mosenthin, R.   +3 more
core   +1 more source

RNA interference is ineffective as a routine method for gene silencing in chick embryos as monitored by fgf8 silencing. [PDF]

, 2013
The in vivo accessibility of the chick embryo makes it a favoured model system for experimental developmental biology. Although the range of available techniques now extends to miss-expression of genes through in ovo electroporation, it remains difficult
Bueno i Torrens, David, 1965-, Hernández Hernández, Victor   +1 more
core   +3 more sources

An efficient and universal protoplast-based transient gene expression system for genome editing in Brassica crops

Horticultural Plant Journal
Protoplast-based transient gene expression system has been widely used in plant genome editing because of its simple operation and less time-consuming.
Xiaoxiao Yu, Jintai Yu, Yin Lu, Wenjing Li, Guanzhong Huo, Jun Zhang, Yan Li, Jianjun Zhao, Jun Li   +8 more
doaj   +1 more source