Results 71 to 80 of about 773,655 (299)

Development of Agrobacterium-mediated transient expression system in Caragana intermedia and characterization of CiDREB1C in stress response

BMC Plant Biology, 2019
Background The Agrobacterium-mediated transient transformation is a versatile and indispensable way of rapid analyzing gene function in plants. Despite this transient expression system has been successfully applied in a number of plant species, it is ...
Kun Liu, Qi Yang, Tianrui Yang, Yang Wu, Guangxia Wang, Feiyun Yang, Ruigang Wang, Xiaofei Lin, Guojing Li   +8 more
doaj   +1 more source

Over-expression of a chimeric gene of the transcriptional co-activator MBF1 fused to the EAR repressor motif causes developmental alteration in Arabidopsis and tomato [PDF]

, 2008
Transcriptional co-activators of the Multiprotein Bridging Factor1 (MBF1) type belong to a multigenic family that encode key components of the machinery controlling gene expression by communicating between transcription factors and the basal ...
Bouzayen, Mondher, Hommel, Marie, Jaimes-Miranda, Fabiola, Khalil-Ahmad, Qaiser, Latché, Alain, Mila, Isabelle, Pech, Jean-Claude, Pouzet, Cécile, Regad, Farid   +8 more
core   +2 more sources

Transient Gene Expression in Tobacco using Gibson Assembly and the Gene Gun [PDF]

Journal of Visualized Experiments, 2014
In order to target a single protein to multiple subcellular organelles, plants typically duplicate the relevant genes, and express each gene separately using complex regulatory strategies including differential promoters and/or signal sequences. Metabolic engineers and synthetic biologists interested in targeting enzymes to a particular organelle are ...
Matthew D, Mattozzi, Mathias J, Voges, Pamela A, Silver, Jeffrey C, Way   +3 more
openaire   +2 more sources

Organ‐specific redox imbalances in spinal muscular atrophy mice are partially rescued by SMN antisense oligonucleotides

FEBS Letters, EarlyView.
We identified a systemic, progressive loss of protein S‐glutathionylation—detected by nonreducing western blotting—alongside dysregulation of glutathione‐cycle enzymes in both neuronal and peripheral tissues of Taiwanese SMA mice. These alterations were partially rescued by SMN antisense oligonucleotide therapy, revealing persistent redox imbalance as ...
Sofia Vrettou, Brunhilde Wirth
wiley   +1 more source

Global characterization of GH11 family xylanases genes in Neostagonosporella sichuanensis and functional analysis of Nsxyn1 and Nsxyn2

Frontiers in Microbiology
Rhombic-spot disease, caused mainly by Neostagonosporella sichuanensis, significantly impacts the yield and quality of fishscale bamboo (Phyllostachys heteroclada).
Lijuan Liu, Lijuan Liu, Chengsong Li, Chengsong Li, Fang Liang, Fang Liang, Shan Han, Shan Han, Shujiang Li, Shujiang Li, Chunlin Yang, Chunlin Yang, Yinggao Liu, Yinggao Liu   +13 more
doaj   +1 more source

Tau acetylation at K331 has limited impact on tau pathology in vivo

FEBS Letters, EarlyView.
We mapped tau post‐translational modifications in humanized MAPT knock‐in mice and in amyloid‐bearing double knock‐in mice. Acetylation within the repeat domain, particularly around K331, showed modest increases under amyloid pathology. To test functional relevance, we generated MAPTK331Q knock‐in mice.
Shoko Hashimoto, Yukio Matsuba, Mika Takahashi, Takaomi C. Saido   +3 more
wiley   +1 more source

Structural insights into an engineered feruloyl esterase with improved MHET degrading properties

FEBS Letters, EarlyView.
A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa, Konstantinos Makryniotis, Theofani‐Iosifina Sousani, Evangelos Topakas, Vangelis Daskalakis, Maria Dimarogona   +5 more
wiley   +1 more source

Transient expression vectors for functional genomics, quantification of promoter activity and RNA silencing in plants

Plant Methods, 2005
Background We describe novel plasmid vectors for transient gene expression using Agrobacterium, infiltrated into Nicotiana benthamiana leaves. We have generated a series of pGreenII cloning vectors that are ideally suited to transient gene expression, by
Templeton Matthew D, Grafton Karryn, Bolitho Karen, Friel Ellen N, Allan Andrew C, Hellens Roger P, Karunairetnam Sakuntala, Gleave Andrew P, Laing William A   +8 more
doaj   +1 more source

Hyperosmotic stress induces PARP1‐mediated HPF1‐dependent mono(ADP‐ribosyl)ation

FEBS Letters, EarlyView.
Sorbitol‐induced hyperosmotic stress rapidly induces reversible mono(ADP‐ribosyl)ation (MARylation) on PARP1 without the signs of genotoxic signaling. We show that PARP1 autoMARylation is HPF1 dependent and forms hydroxylamine‐resistant O‐glycosidic linkages.
Anna Georgina Kopasz, Mihály Mérey, Rebeka Vásárhelyi, Ramóna Pék, Victor Imburchia, László Henn, Adrián Kószó, Nicholas D. Lakin, Ivan Ahel, Sébastien Huet, Ágnes Czibula, Gyula Timinszky   +11 more
wiley   +1 more source

Transient expression efficiency of a novel Agrobacterium strain in soybean leaf tissue [PDF]

, 2015
Agriculture/Environmental Science: 3rd Place (The Ohio State University Denman Undergraduate Research Forum)Agroinfiltration is an efficient tool used to rapidly assess plant gene function by transiently expressing DNA of interest.
Willman, Matthew
core