Results 81 to 90 of about 5,757,325 (187)

clustering of microarray gene expression data

, 2005
Motivation: Cluster analysis of gene expression profiles has been widely applied to clustering genes for gene function discovery. Many approaches have been proposed.
Gene Expression, Wei Pan
core  

A high-efficiency transient expression system mediated by Agrobacterium tumefaciens in Spinacia oleracea leaves

Plant Methods
Background Optimization of a highly efficient transient expression system is critical for the study of gene function, particularly in those plants in which stable transformation methods are not widely available.
Yumeng Zhang, Liuliu Qiu, Yongxue Zhang, Yiran Wang, Chunxiang Fu, Shaojun Dai, Meihong Sun   +6 more
doaj   +1 more source

Development of novel lipoplex formulation methodologies to improve large-scale transient transfection for lentiviral vector manufacture

Molecular Therapy: Methods & Clinical Development
Large-scale transient transfection has advanced significantly over the last 20 years, enabling the effective production of a diverse range of biopharmaceutical products, including viral vectors.
Thomas Williams-Fegredo, Lee Davies, Carol Knevelman, Kyriacos Mitrophanous, James Miskin, Qasim A. Rafiq   +5 more
doaj   +1 more source

Transient gene expression in Medicago truncatula leaves via agroinfiltration

, 2013
Transient expression is a powerful method for the functional characterization of genes. In this chapter, we outline a protocol for the transient expression of constructs in Medicago truncatula leaves using Agrobacterium tumefaciens infiltration.
Picard, K., Kelsey Picard, Hellens, R.P., Lee, R., Macknight, R., Robyn Lee, Roger Hellens, Richard Macknight   +7 more
core   +1 more source

Time saving method for protoplast isolation, transformation and transient gene expression assay in barley

Agricultural and Food Science, 1994
This study was conducted to establish a rapid method for barley (Hordeum vulgare L.) protoplast isolation to provide an easy-to-use procedure for the transformation and primary investigation of new gene constructs by transient gene expression assays ...
Barnabas Jenes, Matti Puolimatka, Pedro Bittencourt, Seppo Pulli   +3 more
doaj   +2 more sources

Combinatorial transient gene expression strategies to enhance terpenoid production in plants. [PDF]

Front Plant Sci, 2022
Park S, Mani V, Kim JA, Lee SI, Lee K.
europepmc   +1 more source

Comparison of different transient gene expression systems for the production of a new humanized anti-HER2 monoclonal antibody (Hersintuzumab). [PDF]

Daru, 2023
Roshani A, Mohammadi M, Bahadori T, Ahmadi Zare H, Judaki MA, Mobini M, Golsaz-Shirazi F, Jeddi-Tehrani M, Amiri MM, Shokri F.   +9 more
europepmc   +1 more source

Cotyledon peeling method for passion fruit protoplasts: a versatile cell system for transient gene expression in passion fruit (Passiflora edulis). [PDF]

Front Plant Sci, 2023
Wang L, Liu H, Liu P, Wu G, Shen W, Cui H, Dai Z.   +6 more
europepmc   +1 more source

Optimization of Isolation and Transformation of Protoplasts from Uncaria rhynchophylla and Its Application to Transient Gene Expression Analysis. [PDF]

Int J Mol Sci, 2023
Shao Y, Mu D, Pan L, Wilson IW, Zheng Y, Zhu L, Lu Z, Wan L, Fu J, Wei S, Song L, Qiu D, Tang Q.   +12 more
europepmc   +1 more source

Establishment of an efficient cotton root protoplast isolation protocol suitable for single-cell RNA sequencing and transient gene expression analysis. [PDF]

Plant Methods, 2023
Zhang K, Liu S, Fu Y, Wang Z, Yang X, Li W, Zhang C, Zhang D, Li J.   +8 more
europepmc   +1 more source