Results 81 to 90 of about 773,655 (299)

Genome analysis and avirulence gene cloning using a high-density RADseq linkage map of the flax rust fungus, Melampsora lini [PDF]

, 2016
Agroinfiltration of avirulence gene constructs. The response of flax cultivars and near-isogenic lines to expression of avirulence gene candidates (AvrM14-A, AvrM14-B and AvrL2-A) using Agrobacterium tumefaciens-mediated transient transformation.
Adnane Nemri, Adrienne R. Hardham, Ann-Maree Catanzariti, Cameron A. Jack, Claire Anderson, David A. Jones, Gregory J. Lawrence, Jeffrey G. Ellis, Muhammad Adil Khan, Narayana M. Upadhyaya, Peter N. Dodds   +10 more
core   +11 more sources

Moment-based inference predicts bimodality in transient gene expression [PDF]

Proceedings of the National Academy of Sciences, 2012
Recent computational studies indicate that the molecular noise of a cellular process may be a rich source of information about process dynamics and parameters. However, accessing this source requires stochastic models that are usually difficult to analyze.
Zechner Christoph, Ruess Jakob, Krenn Peter, Pelet Serge, Peter Matthias, Lygeros John, Koeppl Heinz   +6 more
openaire   +4 more sources

An isoform of 14‐3‐3 protein regulates transbilayer lipid movement at the plasma membrane

FEBS Letters, EarlyView.
Loss of 14‐3‐3ζ in CHO cells confers resistance to exogenous phosphatidylserine (PS) and impairs endocytosis‐independent inward flip‐flop of fluorescent PS at the plasma membrane. RNAi‐mediated knockdown reproduces this defect, while no additive effect is seen in ATP11C‐deficient cells.
Akiko Yamaji‐Hasegawa, Akira Hattori, Masafumi Tsujimoto, Toshihide Kobayashi   +3 more
wiley   +1 more source

Transient reprogramming of postnatal cardiomyocytes to a dedifferentiated state.

PLoS ONE, 2021
In contrast to mammals, lower vertebrates are capable of extraordinary myocardial regeneration thanks to the ability of their cardiomyocytes to undergo transient dedifferentiation and proliferation.
Thomas Kisby, Irene de Lázaro, Maria Stylianou, Giulio Cossu, Kostas Kostarelos   +4 more
doaj   +1 more source

Stabilization of GFP Expression in a Transient Expression System using Viral Suppressors of Silencing [PDF]

, 2007
Using transient expression systems, introduction of the green fluorescent protein (gfp) gene results in peak GFP expression 24 hrs post-bombardment (hpb), followed by a rapid decline within 72 hpb.
Dhillon, Taniya
core  

Assessment of RNAi-induced silencing in banana (Musa spp.) [PDF]

, 2015
In plants, RNA- based gene silencing mediated by small RNAs functions at the transcriptional or post-transcriptional level to negatively regulate target genes, repetitive sequences, viral RNAs and/or transposon elements.
Cammue, B.P.A., Coninck, B. de, Dang Thi Tuong Vi, Henry, I., Remy, S., Swennen, Rony L., Windelinckx, S.   +6 more
core   +1 more source

Transcriptional network analysis of PTEN‐protein‐deficient prostate tumors reveals robust stromal reprogramming and signs of senescent paracrine communication

Molecular Oncology, EarlyView.
Combining PTEN protein assessment and transcriptomic profiling of prostate tumors, we uncovered a network enriched in senescence and extracellular matrix (ECM) programs associated with PTEN loss and conserved in a mouse model. We show that PTEN‐deficient cells trigger paracrine remodeling of the surrounding stroma and this information could help ...
Ivana Rondon‐Lorefice, Jose I. Lopez, Aitziber Ugalde‐Olano, Maite Zufiaurre, Ianire Astobiza, Natalia Martin‐Martin, Laura Bozal‐Basterra, Saioa Garcia‐Longarte, Amaia Zabala‐Letona, Sofia Rey, Aida Santos‐Martin, Miguel Unda, Ana Loizaga‐Iriarte, Mariona Graupera, Paolo Nuciforo, Arkaitz Carracedo, Isabel Mendizabal   +16 more
wiley   +1 more source

Impact of polyplex micelles installed with cyclic RGD peptide as ligand on gene delivery to vascular lesions [PDF]

, 2010
Gene therapy is expected to open a new strategy for the treatment of refractory vascular diseases, so the development of appropriate gene vectors for vascular lesions is needed. To realize this requirement with a non-viral approach, cyclo(RGDfK) peptide (
Hideo Kagaya, Hiroyuki Koyama, Kazunori Kataoka, Makoto Oba, Takehiko Ishii, Tetsuro Miyata, Tsuyoshi Takato, Yutaka Miura   +7 more
core   +1 more source

Potential therapeutic targeting of BKCa channels in glioblastoma treatment

Molecular Oncology, EarlyView.
This review summarizes current insights into the role of BKCa and mitoBKCa channels in glioblastoma biology, their potential classification as oncochannels, and the emerging pharmacological strategies targeting these channels, emphasizing the translational challenges in developing BKCa‐directed therapies for glioblastoma treatment.
Kamila Maliszewska‐Olejniczak, Karolina Pytlak, Sandra Jaworowska, Bogusz Kulawiak, Piotr Bednarczyk   +4 more
wiley   +1 more source

Simplified Protocol to Demonstrate Gene Expression in Nicotiana benthamiana Using an Agrobacterium-Mediated Transient Assay

Bio-Protocol
Agrobacterium-mediated transient gene expression in Nicotiana benthamiana is widely used to study gene function in plants. One dramatic phenotype that is frequently screened for is cell death.
Satyam Vergish , Ryan Wolf, Wen- Song
doaj   +1 more source