Results 101 to 110 of about 6,113,361 (250)

USP29‐regulated noncanonical stabilization of the hypoxia‐inducible factor‐α in aggressive prostate cancer

Molecular Oncology, EarlyView.
We identify USP29 as the only DUB mirroring CA9 expression, a marker of hypoxia and HIF pathway activation associated with PCA aggressiveness. USP29 stabilizes HIF‐1α and HIF‐2α via a noncanonical mechanism that is independent of PHD/pVHL activity yet relies on proteasomal regulation, establishing USP29 as a previously unrecognized regulator of hypoxic
Amelie S Schober, Leire Moreno‐Cugnon, Laura Martínez‐Pérez, Amaia Ercilla, Amaia Zabala‐Letona, Adrián Vicente‐Barrueco, Maider Fagoaga‐Eugui, Saioa Garcia‐Longarte, Blanca Calle‐Ciborro, Encarnación Pérez‐Andrés, Onintza Carlevaris, Sara Pozo, Isabel Mendizabal, Ugo Mayor, Arkaitz Carracedo, Violaine Sée, Edurne Berra   +16 more
wiley   +1 more source

A hybrid lentivirus-transposon vector for safer gene therapy [PDF]

, 2010
Gene therapy vectors based on the HIV-1 lentivirus are an attractive option for clinical applications because they enter a broad range of target cells efficiently and deliver stable gene expression through integration into host chromosomes.
Vink, C.A.
core  

Cloning of the Complete Gene for Carcinoembryonic Antigen [PDF]

, 1990
Carcinoembryonic antigen (CEA) is a widely used tumor marker, especially in the surveillance of colonic cancer patients. Although CEA is also present in some normal tissues, it is apparently expressed at higher levels in tumorous tissues than in ...
Schrewe, Heinrich, Bona, Marion, Zimmermann, Wolfgang, Shively, John E., Hassauer, Martin, Hefta, Laura J. F., Maruya, Aiko, Thompson, John A., Kleist, Sabine von   +8 more
core   +1 more source

An efficient and universal protoplast-based transient gene expression system for genome editing in Brassica crops

Horticultural Plant Journal
Protoplast-based transient gene expression system has been widely used in plant genome editing because of its simple operation and less time-consuming.
Xiaoxiao Yu, Jintai Yu, Yin Lu, Wenjing Li, Guanzhong Huo, Jun Zhang, Yan Li, Jianjun Zhao, Jun Li   +8 more
doaj   +1 more source

Oncogenic DMTF1β promotes cancer cell motility by regulating autophagy through ULK1 stabilization

Molecular Oncology, EarlyView.
In the current study, we demonstrate that the oncogene DMTF1β regulates ULK1 stability by reducing its proteasomal degradation in cancer cells. This stabilization enables ULK1 to induce autophagy, which in turn facilitates cancer cell migration. Consequently, reduced DMTF1β levels lead to decreased autophagy and impaired cancer cell migration.
Jun Xu, Nicolas J. Niklaus, Anna M. Schläfli, Igor Tokarchuk, Magali Humbert, Federico La Manna, Bich Vu, David Maul, Ramin Radpour, Marianna Kruithof‐de Julio, Inti Zlobec, Jörn Dengjel, Bruce E. Torbett, Mario P. Tschan   +13 more
wiley   +1 more source

Chromatin Structures of the Rat Tyrosine Aminotransferase Gene Relate to the Function of Its cis-Acting Elements [PDF]

, 1990
The relationship between DNase I-hypersensitive sites (HSs) and transcriptional enhancers of the rat tyrosine aminotransferase (TAT) gene was examined by comparing HSs in and around the TAT gene with the activity of the corresponding DNA sequences in ...
Boshart, Michael, Schütz, Günther, Nitsch, Doris, Mestril, Ruben, Weih, Falk, Stewart, A. F.   +5 more
core   +1 more source

Loss of proton‐sensing TDAG8 increases tumor progression in mouse models of colon cancer

Molecular Oncology, EarlyView.
Loss of the pH‐sensing receptor TDAG8 accelerates colorectal cancer progression in mice. Animals lacking TDAG8 expression had increased tumor growth, DNA damage, and recruitment of tumor‐associated immune cells, including macrophages, neutrophils, and monocytes.
Ermanno Malagola, Yasmine Illi, Anna Bircher, Marijn Wilmink, Rachele F. Malagutti, Solenn Ottié, Cordelia Schuler, Pedro A. Ruiz, Cheryl de Vallière, Klaus Seuwen, Gerhard Rogler, Martin Hausmann   +11 more
wiley   +1 more source

Fuzzy Clustering Models for Gene Expression Data Analysis [PDF]

With the advent of microarray technology, it is possible to monitor gene expression of tens of thousands of genes in parallel. In order to gain useful biological knowledge, it is necessary to study the data and identify the underlying patterns, which ...
Wang, Yu
core  

A high-efficiency transient expression system mediated by Agrobacterium tumefaciens in Spinacia oleracea leaves

Plant Methods
Background Optimization of a highly efficient transient expression system is critical for the study of gene function, particularly in those plants in which stable transformation methods are not widely available.
Yumeng Zhang, Liuliu Qiu, Yongxue Zhang, Yiran Wang, Chunxiang Fu, Shaojun Dai, Meihong Sun   +6 more
doaj   +1 more source

Development of novel lipoplex formulation methodologies to improve large-scale transient transfection for lentiviral vector manufacture

Molecular Therapy: Methods & Clinical Development
Large-scale transient transfection has advanced significantly over the last 20 years, enabling the effective production of a diverse range of biopharmaceutical products, including viral vectors.
Thomas Williams-Fegredo, Lee Davies, Carol Knevelman, Kyriacos Mitrophanous, James Miskin, Qasim A. Rafiq   +5 more
doaj   +1 more source