Results 11 to 20 of about 7,887 (237)
Agroinfiltration contributes to VP1 recombinant protein degradation [PDF]
Bioengineered, 2016 There is a growing interest in applying tobacco agroinfiltration for recombinant protein production in a plant based system. However, in such a system, the action of proteases might compromise recombinant protein production. Protease sensitivity of model recombinant foot-and-mouth disease (FMD) virus P1-polyprotein (P1) and VP1 (viral capsid protein 1)
Priyen Pillay +2 more
exaly +5 more sources
Initiation of Plant Virus Infections by Agroinfiltration of Infectious Viral cDNAs (icDNAs).
Methods in molecular biology, 2023 Agroinfiltration uses Agrobacterium to deliver T-DNA-based gene expression constructs into plants. This chapter focuses on the standard method, specifically from the perspective of plant virus research, and describes a protocol for the initiation of virus infections in plants via infiltration of Agrobacterium strains carrying infectious viral cDNAs ...
Maija Pollari
semanticscholar +5 more sources
Agroinfiltration-mediated transient assay for rapid evaluation of constructs in pigeonpea [PDF]
Biotechnology NotesThe process of generating stable transformants is time-consuming, labor-intensive, and genotype-dependent. In contrast, transient gene expression techniques, such as agroinfiltration, offer a rapid assessment of gene function and expression ...
K. Yogendra +3 more
semanticscholar +4 more sources
Within leaf variation is the largest source of variation in agroinfiltration of Nicotiana benthamiana [PDF]
Plant Methods, 2015 Transient gene expression utilizing syringe agroinfiltration offers a simple and efficient technique for different transgenic applications. Leaves of Nicotiana benthamiana show reliable and high transformation efficiency, but in quantitative assays also a certain degree of variation.
Hany Bashandy, Teemu Heikki Teeri
exaly +6 more sources
PLoS ONE, 2023 Cowpea (Vigna unguiculata) is a legume staple widely grown across Sub-Saharan Africa and other tropical and sub-tropical regions. Considering projected climate change and global population increases, cowpea’s adaptation to hot climates, resistance to ...
Aya Bridgeland +4 more
semanticscholar +2 more sources
In-planta transient transformation of avocado (Persea americana) by vacuum agroinfiltration of aerial plant parts [PDF]
Plant Cell Tissue and Organ Culture, 2023 Leaf age, wounding and jasmonic acid counter the recalcitrant nature of avocado, unlocking the use of Agrobacterium mediated transient transformation as a tool for functional genetics in this plant.
J. A. Salazar-González +5 more
semanticscholar +2 more sources
Agroinfiltration Mediated Scalable Transient Gene Expression in Genome Edited Crop Plants
International Journal of Molecular Sciences, 2021 Agrobacterium-mediated transformation is one of the most commonly used genetic transformation method that involves transfer of foreign genes into target plants. Agroinfiltration, an Agrobacterium-based transient approach and the breakthrough discovery of
M. Kaur +6 more
semanticscholar +2 more sources
Depletion of the Nb CORE receptor drastically improves agroinfiltration productivity in older Nicotiana benthamiana plants [PDF]
bioRxiv, 2023 Nicotiana benthamiana is increasingly used for transient gene expression to produce antibodies, vaccines, and other pharmaceutical proteins but transient gene expression is low in fully developed, 6-8wk old plants.
Isobel Dodds +3 more
semanticscholar +2 more sources
Molecular Plant-Microbe Interactions, 2000 The avirulence genes Avr9 and Avr4 from the fungal tomato pathogen Cladosporium fulvum encode extracellular proteins that elicit a hypersensitive response when injected into leaves of tomato plants carrying the matching resistance genes, Cf-9 and Cf-4 ...
Renier A. L. Van der Hoorn +3 more
doaj +2 more sources
Boosting lactoferrin yields in Nicotiana tabacum via HC-Pro co-agroinfiltration. [PDF]
BMC Plant BiolIntroduction Plant-based recombinant protein production provides a safe and cost-effective method for producing functional medicinal proteins. However, transgene silencing, a natural defense mechanism in plants, often reduces protein production by ...
Lotfi M +11 more
europepmc +2 more sources