Results 11 to 20 of about 8,861 (240)
Agroinfiltration-mediated transient assay for rapid evaluation of constructs in pigeonpea [PDF]
Biotechnology NotesThe process of generating stable transformants is time-consuming, labor-intensive, and genotype-dependent. In contrast, transient gene expression techniques, such as agroinfiltration, offer a rapid assessment of gene function and expression. Agroinfiltration, widely employed for studying gene function, has been extensively applied in leaf tissues of ...
Kalenahalli Yogendra, Wricha Tyagi
exaly +4 more sources
Improving agroinfiltration-based transient gene expression in Nicotiana benthamiana [PDF]
Plant Methods, 2018 Background Agroinfiltration is a simple and effective method of delivering transgenes into plant cells for the rapid production of recombinant proteins and has become the preferred transient expression platform to manufacture biologics in plants. Despite
Karlah Norkunas +3 more
doaj +4 more sources
Agroinfiltration technique for elucidating the functions of strawberry genes in Fragaria vesca. [PDF]
Sci RepAbstract Unraveling gene function is crucial for enhancing quality traits in commercially cultivated strawberry (Fragaria × ananassa) through biotechnology. However, such analysis is hindered by the complexity of the octoploid genome (2n = 8x = 56) of F. × ananassa.
Thagun C, Kodama Y.
europepmc +3 more sources
Frontiers in Plant Science, 2021 Plant-based expression platforms are currently gaining acceptance as a viable alternative for the production of recombinant proteins (RPs), but the degradation of RPs by proteases in cells hinders their superb potentials.
Jiexue Ma +7 more
doaj +1 more source
Leaping into the Unknown World of Sporisorium scitamineum Candidate Effectors
Journal of Fungi, 2020 Sporisorium scitamineum is a biotrophic fungus causing sugarcane smut disease. In this study, we set up a pipeline and used genomic and dual transcriptomic data previously obtained by our group to identify candidate effectors of S.
Natália Sousa Teixeira-Silva +6 more
doaj +1 more source
Unraveling Plant Cell Death during Phytophthora Infection
Microorganisms, 2022 Oomycetes form a distinct phylogenetic lineage of fungus-like eukaryotic microorganisms, of which several hundred organisms are considered among the most devastating plant pathogens—especially members of the genus Phytophthora. Phytophthora spp.
Kayla A. Midgley +2 more
doaj +1 more source
Leaf-Encapsulated Vaccines: Agroinfiltration and Transient Expression of the Antigen Staphylococcal Endotoxin B in Radish Leaves. [PDF]
, 2018 Transgene introgression is a major concern associated with transgenic plant-based vaccines. Agroinfiltration can be used to selectively transform nonreproductive organs and avoid introgression.
Gallo, Richard L +6 more
core +3 more sources
Plant Direct, 2020 Hessian fly Mayetiola destructor is a notorious pest of wheat. Previous studies suggest that Hessian fly uses effector‐based mechanisms to attack wheat plants during parasitism, but no direct evidence has been reported to support this postulation.
Zainab Aljbory +4 more
doaj +1 more source
PLoS ONE, 2022 Agroinfiltration is a method used in biopharming to support plant-based biosynthesis of therapeutic proteins such as antibodies and viral antigens involved in vaccines.
Jordan Demone +7 more
doaj +1 more source
Pathogen manipulation of chloroplast function triggers a light-dependent immune recognition [PDF]
, 2020 In plants and animals, nucleotide-binding leucine-rich repeat (NLR) proteins are intracellular immune sensors that recognize and eliminate a wide range of invading pathogens.
Bozkurt, Tolga O. +13 more
core +1 more source