Results 31 to 40 of about 8,861 (240)

Transient Gene Expression: an Approach for Recombinant Vaccine Production [PDF]

Journal of Medical Microbiology and Infectious Diseases, 2021
The production of recombinant vaccines in green plants is an attractive and promising topic in genetic engineering. However, the stable transformation of green plants is a time-consuming, costly, and labor-intensive practice.
Maziar Habibi-Pirkoohi, Amir Ghaffar Shahriari, Mohamad Hamed Ghodoum Parizipour   +2 more
doaj   +1 more source

Screening of Secreted Proteins of Sporisorium reilianum f. sp. zeae for Cell Death Suppression in Nicotiana benthamiana

Frontiers in Plant Science, 2020
Sporisorium reilianum f. sp. zeae (SRZ) is a biotrophic fungus causing head smut in maize. Maize infection with SRZ leads to very little cell death suggesting the presence of cell-death suppressinpg effectors.
Deiziane Dutra, Nisha Agrawal, Nisha Agrawal, Hassan Ghareeb, Hassan Ghareeb, Jan Schirawski, Jan Schirawski, Jan Schirawski   +7 more
doaj   +1 more source

Transient Gene Expression for the Characteristic Signal Sequences and the Estimation of the Localization of Target Protein in Plant Cell

Bio-Protocol, 2018
We have proposed and tested a method for characterization of the signal sequences and determinations of target protein localization in a plant cell.
Mikhail Berestovoy, Alexander Tyurin, Ksenia Kabardaeva, Yuriy Sidorchuk, Artem Fomenkov, Alexander Nosov, Irina Goldenkova-Pavlova   +6 more
doaj   +1 more source

The production of Newcastle disease virus-like particles in Nicotiana benthamiana as potential vaccines

Frontiers in Plant Science, 2023
Newcastle disease (ND) is a highly contagious viral respiratory and neurological disease that has a severe impact on poultry production worldwide. In the present study, an expression platform was established for the transient production in N.bethamiana ...
Tanja Smith, Tanja Smith, Martha M. O’Kennedy, Martha M. O’Kennedy, Craig S. Ross, Nicola S. Lewis, Nicola S. Lewis, Celia Abolnik   +7 more
doaj   +1 more source

Visualization of plant viral suppressor silencing activity in intact leaf lamina by quantitative fluorescent imaging [PDF]

, 2011
Publication of this article was funded by the Stellenbosch University Open Access Fund.The original publication is available at http://www.plantmethods.com/content/7/1/25ABSTRACT: BACKGROUND: Transient expression of proteins in plants has become a ...
Coba Slabber, Dirk Stephan, Gavin George, Johan T Burger, Kevin P Francis, Victor Ninov   +5 more
core   +4 more sources

Comparative Evaluation of Transient Protein Expression Efficiency in Tissues across Soybean Varieties Using the Tsukuba System

Plants
Transient protein expression is a versatile tool with diverse applications and can be used in soybeans to study gene function, obtain mutants, and produce proteins for commercial use.
Martina Bianca Fuhrmann-Aoyagi, Saki Igarashi, Kenji Miura   +2 more
doaj   +1 more source

Combinatorial transient gene expression strategies to enhance terpenoid production in plants

Frontiers in Plant Science, 2022
IntroductionThe monoterpenoid linalool and sesquiterpenoid costunolide are ubiquitous plant components that have been economically exploited for their respective essential oils and pharmaceutical benefits.
Soyoung Park, Vimalraj Mani, Jin A. Kim, Soo In Lee, Kijong Lee   +4 more
doaj   +1 more source

A conditional silencing suppression system for transient expression. [PDF]

, 2018
RNA silencing is a powerful tool deployed by plants against viral infection and abnormal gene expression. Plant viruses have evolved a suite of silencing suppressors for counter-defense, which are also widely used to boost transcript and protein ...
Bally, J., Litholdo, C.G., Reis, R.S., Roberts, T.H., Waterhouse, P.M.   +4 more
core   +1 more source

Improving transient gene expression and agroinfiltration‐based transformation effectiveness in Indonesian orchid Phalaenopsis amabilis (L.) Blume

Indonesian Journal of Biotechnology
Transient gene expression is an approach used to study transient genes across various species, with infiltration by Agrobacterium tumefaciens (agroinfiltration) being a commonly used method.
Dionysia Heviarie Primasiwi, Yekti Asih Purwestri, Endang Semiarti   +2 more
doaj   +1 more source

Agroinfiltration Is a Versatile Tool That Facilitates Comparative Analyses of Avr9/Cf-9-Induced and Avr4/Cf-4-Induced Necrosis

Molecular Plant-Microbe Interactions, 2000
The avirulence genes Avr9 and Avr4 from the fungal tomato pathogen Cladosporium fulvum encode extracellular proteins that elicit a hypersensitive response when injected into leaves of tomato plants carrying the matching resistance genes, Cf-9 and Cf-4 ...
Renier A. L. Van der Hoorn, Franck Laurent, Ronelle Roth, Pierre J. G. M. De Wit   +3 more
doaj   +1 more source