Results 51 to 60 of about 8,861 (240)

Transient Expression of Homologous Hairpin RNA Causes Interference with Plant Virus Infection and Is Overcome by a Virus Encoded Suppressor of Gene Silencing

Molecular Plant-Microbe Interactions, 2003
Specific post-transcriptional gene silencing (PTGS) of target genes can be induced in a variety of organisms by providing homologous double-stranded RNA (dsRNA) molecules. In plants, PTGS is part of a defense mechanism against virus infection.
F. Tenllado, D. Barajas, M. Vargas, F. A. Atencio, P. González-Jara, J. R. Díaz-Ruíz   +5 more
doaj   +1 more source

Structures of Phytophthora RXLR Effector Proteins: a conserved but adaptable fold underpins functional diversity [PDF]

, 2011
Phytopathogens deliver effector proteins inside host plant cells to promote infection. These proteins can also be sensed by the plant immune system, leading to restriction of pathogen growth.
Adams, Allen, Aminetzach, Armstrong, Bailey, Baxter, Bos, Bos, Bos, Bos, Cowtan, Davis, Demeler, Dodds, Dou, Eddy, Emsley, Evans, Haas, Holm, Jiang, Jones, Karimi, Kearney, Kim, Kobe, Krissinel, Krissinel, Larkin, Leslie, Lindbo, Liu, Markson, McCoy, Nomura, Oh, Potterton, Raffaele, Samanta, Schmidt, Schornack, Schornack, Shan, Sheldrick, Tyler, Vleeshouwers, Whisson, Wierenga, Win, Yang   +49 more
core   +1 more source

Efficient Agroinfiltration of Plants for High-level Transient Expression of Recombinant Proteins [PDF]

Journal of Visualized Experiments, 2013
Mammalian cell culture is the major platform for commercial production of human vaccines and therapeutic proteins. However, it cannot meet the increasing worldwide demand for pharmaceuticals due to its limited scalability and high cost. Plants have shown to be one of the most promising alternative pharmaceutical production platforms that are robust ...
Kahlin, Leuzinger, Matthew, Dent, Jonathan, Hurtado, Jake, Stahnke, Huafang, Lai, Xiaohong, Zhou, Qiang, Chen   +6 more
openaire   +2 more sources

Transposase‐Assisted Donor Tethering Boosts Large‐Fragment HDR in Plants

Advanced Science, EarlyView.
A transposase‐assisted donor tethering strategy is developed to enhance homology‐directed repair in plants. By recruiting donor DNA to double‐strand breaks and synergizing with repair pathway reprogramming and transcription‐coupled donor design, this system markedly improves large‐fragment targeted insertion efficiency, providing a robust platform for ...
Sha Wei, Kai Zhang, Shuke Deng, Jinjie Chen, Xiaomei Huang, Jingfei Guo, Yuqing Wu, Yingjie Guo, Zhen Liang   +8 more
wiley   +1 more source

pSITE Vectors for Stable Integration or Transient Expression of Autofluorescent Protein Fusions in Plants: Probing Nicotiana benthamiana-Virus Interactions

Molecular Plant-Microbe Interactions, 2007
Plant functional proteomics research is increasingly dependent upon vectors that facilitate high-throughput gene cloning and expression of fusions to autofluorescent proteins.
Romit Chakrabarty, Rituparna Banerjee, Sang-Min Chung, Mark Farman, Vitaly Citovsky, Saskia A. Hogenhout, Tzvi Tzfira, Michael Goodin   +7 more
doaj   +1 more source

Enabling the study of gene function in gymnosperms: Virus‐induced gene silencing in Ephedra tweedieana

Applications in Plant Sciences, EarlyView.
Abstract Premise As the sister clade to angiosperms, extant gymnosperms are crucial for reconstructing ancestral gene regulatory networks in seed plants. This highlights the need for model systems representing each of their distinct lineages. However, tools to quickly and effectively investigate gene function in gymnosperms are still limited due to the
Anthony G. K. Garcia, Jo Trang Bùi, Todd P. Michael, Stefanie M. Ickert‐Bond, Verónica S. Di Stilio   +4 more
wiley   +1 more source

An efficient system to detect protein ubiquitination by agroinfiltration inNicotiana benthamiana [PDF]

The Plant Journal, 2010
The ubiquitination proteasome pathway has been demonstrated to regulate all plant developmental and signaling processes. E3 ligase/substrate-specific interactions and ubiquitination play important roles in this pathway. However, due to technical limitations only a few instances of E3 ligase-substrate binding and protein ubiquitination in plants have ...
Lijing, Liu, Yiyue, Zhang, Sanyuan, Tang, Qingzhen, Zhao, Zhonghui, Zhang, Huawei, Zhang, Li, Dong, Huishan, Guo, Qi, Xie   +8 more
openaire   +2 more sources

The regulatory frameworks surrounding CRISPR‐edited papaya and their impact on international commerce

Journal of the Science of Food and Agriculture, EarlyView.
Abstract The papaya tree (Carica papaya L.), native to the Americas, is cultivated in tropical regions and holds substantial economic importance, with an estimated export volume of 365 000 t in 2023. However, diseases caused by viruses, fungi, bacteria, and nematodes can lead to severe losses.
Luíza Favaratto, Mirielson L da Silva, David S Buss, Oeber F Quadros, Raul Tapia‐Tussell, José A Ventura, Antonio Alberto R Fernandes, Patricia M B Fernandes   +7 more
wiley   +1 more source

Nicotiana benthamiana: Its History and Future as a Model for Plant–Pathogen Interactions

Molecular Plant-Microbe Interactions, 2008
Nicotiana benthamiana is the most widely used experimental host in plant virology, due mainly to the large number of diverse plant viruses that can successfully infect it. Additionally, N.
Michael M. Goodin, David Zaitlin, Rayapati A. Naidu, Steven A. Lommel   +3 more
doaj   +1 more source

Production of Genistein in Amaranthus tricolor var. tristis and Spinacia oleracea by Expression of Glycine max Isoflavone Synthase

Plants, 2021
Isoflavonoids, the diverse group of secondary metabolites derived from the phenylpropanoid pathway, are distributed predominantly in leguminous plants.
Ashwini Malla, Balamurugan Shanmugaraj, Ashutosh Sharma, Sathishkumar Ramalingam   +3 more
doaj   +1 more source