Results 51 to 60 of about 7,887 (237)

Agroinfiltration and PVX Agroinfection in Potato and Nicotiana benthamiana

, 2014
Agroinfiltration and PVX agroinfection are two efficient transient expression assays for functional analysis of candidate genes in plants. The most commonly used agent for agroinfiltration is Agrobacterium tumefaciens, a pathogen of many dicot plant ...
Vleeshouwers, V.G.A.A., Rietman, H., Du, J.   +2 more
core   +1 more source

RAP Tag and PMab-2 Antibody: A Tagging System for Detecting and Purifying Proteins in Plant Cells

Frontiers in Plant Science, 2020
An affinity tag system requires both high affinity and specificity. The RAP tag epitope DMVNPGLEDRIE, derived from rat podoplanin (PDPN), is specifically recognized by PMab-2 monoclonal antibodies in rats. Here, we demonstrated that high levels of PMab-2
Kenji Miura, Kenji Miura, Hideki Yoshida, Hideki Yoshida, Shohei Nosaki, Shohei Nosaki, Mika K. Kaneko, Yukinari Kato, Yukinari Kato   +8 more
doaj   +1 more source

Efficient Agroinfiltration of Plants for High-level Transient Expression of Recombinant Proteins [PDF]

Journal of Visualized Experiments, 2013
Mammalian cell culture is the major platform for commercial production of human vaccines and therapeutic proteins. However, it cannot meet the increasing worldwide demand for pharmaceuticals due to its limited scalability and high cost. Plants have shown to be one of the most promising alternative pharmaceutical production platforms that are robust ...
Kahlin, Leuzinger, Matthew, Dent, Jonathan, Hurtado, Jake, Stahnke, Huafang, Lai, Xiaohong, Zhou, Qiang, Chen   +6 more
openaire   +2 more sources

Schematic diagram of forming agroinfiltration bubble by injecting agroinfiltration liquid into the interface between adaxial epidermis and mesophyll of onion bulb scale.

, 2014
(A) Syringe. (B) Agroinfiltration liquid. (C) Mesophyll of onion bulb scale. (D) Adaxial epidermis of onion bulb scale.
Yi Zhang (9093), Kedong Xu (506662), Manman Wu (506663), Kun Liu (148201), Fuli Zhang (506665), Chengwei Li (181253), Yunxia Chang (506664), Ruiyue Wang (506667), Xiaohui Huang (458909), Yan Wang (15435), Liming Yi (506666), Ju Zhang (281062), Tingting Li (10553), Guangxuan Tan (506668)   +13 more
core   +1 more source

Agroinfiltration for transient gene expression and characterisation of fungal pathogen effectors in cool-season grain legume hosts

Plant Cell Reports, 2021
Modified pEAQ-HT-DEST1 vectors were used for agroinfiltration in legumes. We demonstrate protein expression and export in pea, lentil, and faba bean; however, the method for chickpea was not successful.
J. Debler, Bernadette M. Henares, Robert C. Lee   +2 more
semanticscholar   +1 more source

DNA Methylation Suppression by Bhendi Yellow Vein Mosaic Virus

Epigenomes, 2018
Bhendi yellow vein mosaic virus (BYVMV) belongs to the monopartite begomovirus associated with the β satellite. As a single-stranded DNA (ssDNA) virus, it should be amenable to transcriptional and post-transcriptional gene silencing (TGS and PTGS ...
Kandhalu Sagadevan Dinesh Babu, Ashirbad Guria, Jeyalakshmi Karanthamalai, Nagesh Srikakulam, Kamlesh Kumari, Priyanka Sharma, Sam Aldrin Chandran, Anburaj Daniel Barnabas, Jebasingh Tennyson, Gopal Pandi   +9 more
doaj   +1 more source

Ferritin as a Nanoparticle Scaffold for Plant‐Produced Next‐Generation Subunit Vaccines

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Protein nanoparticles offer an innovative approach to next‐generation subunit vaccine development by displaying antigenic sequences on the nanoparticle surface. Compared to traditional subunit vaccines, protein nanoparticle vaccines often show improved interaction with the immune system due to their particulate size and repetitive epitope ...
Jordan T. VanderBurgt, Richard Strasser, Christopher P. Garnham, Rima Menassa   +3 more
wiley   +1 more source

Expression, Purification and Initial Characterization of Functional α1-Microglobulin (A1M) in Nicotiana benthamiana

Frontiers in Plant Science, 2020
α1-Microglobulin (A1M) is a small glycoprotein that belongs to the lipocalin protein family. A major biological role of A1M is to protect cells and tissues against oxidative damage by clearing free heme and reactive oxygen species.
Magnus L. R. Carlsson, Amanda Kristiansson, Jesper Bergwik, Selvaraju Kanagarajan, Leif Bülow, Bo Åkerström, Li-Hua Zhu   +6 more
doaj   +1 more source

High efficiency of agroinfiltration approach in Phalaenopsis amabilis (L.) Blume (moth orchid) with transient expression of green fluorescent protein reporter gene

Plant Science Today
Agroinfiltration has become a crucial technique in molecular biology, enabling the transfer of functional genes into target plants for rapid gene expression analysis.
P. D. Kasi, E. Gildantia, Y. R. Hanifa, Purnomo, Maryani, E. Semiarti   +5 more
semanticscholar   +1 more source

Identifying resistance‐conferring cytochrome P450s in weeds: experimental strategies and practical considerations

Pest Management Science, EarlyView.
Identifying resistance‐conferring cytochrome P450s requires integrating weed characterization, gene discovery and functional validation. No single approach is sufficient to distinguish causal resistance genes from herbicide‐metabolizing enzymes. Abstract Cytochrome P450 monooxygenases (P450s) play a pivotal role in metabolic herbicide resistance, yet ...
Satoshi Iwakami, Takuya Yamaguchi
wiley   +1 more source