Results 61 to 70 of about 7,887 (237)

Agroinfiltration of grapevine leaves for fast transient assays of gene expression and for long-term production of stable transformed cells [PDF]

, 2008
Agrobacterium-mediated transient assays for the analysis of gene function are used as alternatives to genetic complementation and stable plant transformation.
ZOTTINI, MICHELA, E. Barizza, Michela Zottini, Elisabetta Barizza, COSTA, ALEX, C. Ruberti, A. Costa, M. Zottini, LO SCHIAVO, FIORELLA, Elide Formentin, BARIZZA, ELISABETTA, F. Lo Schiavo, FORMENTIN, ELIDE, Alex Costa, Francesco Carimi, RUBERTI, CRISTINA, Carimi C, Fiorella Lo Schiavo, F. Carimi, Cristina Ruberti, E. Formentin   +20 more
core   +1 more source

Immunization with the Malaria Diversity-Covering Blood-Stage Vaccine Candidate Plasmodium falciparum Apical Membrane Antigen 1 DiCo in Complex with Its Natural Ligand PfRon2 Does Not Improve the In Vitro Efficacy

Frontiers in Immunology, 2017
The blood-stage malaria vaccine candidate Plasmodium falciparum apical membrane antigen 1 (PfAMA1) can induce strong parasite growth-inhibitory antibody responses in animals but has not achieved the anticipated efficacy in clinical trials.
Holger Spiegel, Alexander Boes, Rolf Fendel, Andreas Reimann, Stefan Schillberg, Stefan Schillberg, Rainer Fischer, Rainer Fischer, Rainer Fischer   +8 more
doaj   +1 more source

Functional Characterization of Genes Coding for Novel β-D-Glucosidases Involved in the Initial Step of Secoiridoid Glucosides Catabolism in Centaurium erythraea Rafn

Frontiers in Plant Science, 2022
Secoiridoid glucosides (SGs) are monoterpenoids derived from the iridoid cyclopentane-C-pyran skeleton with β-D glucose linked at C1 position. Coordinated metabolic processes, such as biosynthesis and catabolism of SGs, ensure constitutive presence of ...
Jelena Božunović, Milica Milutinović, Neda Aničić, Marijana Skorić, Dragana Matekalo, Suzana Živković, Milan Dragićević, Biljana Filipović, Tijana Banjanac, Luka Petrović, Danijela Mišić   +10 more
doaj   +1 more source

Improving transient protein expression in agroinfiltrated Nicotiana benthamiana

New Phytologist
SummaryAgroinfiltration of Nicotiana benthamiana is routinely used in plant science and molecular pharming to transiently express proteins of interest. Here, we discuss four phenomena that should be avoided to improve transient expression. Immune responses can be avoided by depleting immune receptors and employing pathogen‐derived effectors; transcript
Beritza, K, Watts, EC, van der Hoorn, RAL   +2 more
openaire   +3 more sources

Simultaneous modification of Thermotoga maritima encapsulin subunits to produce multivalent nanoparticle vaccines for non‐typhoidal Salmonella enterica

The FEBS Journal, EarlyView.
This study investigates the modularity of encapsulin—a protein nanoparticle—by simultaneously inserting up to four distinct Salmonella epitopes within the encapsulin subunit. The encapsulin variants were transiently expressed in plants where they assembled into nanoparticles; however, their accumulation and stability varied depending on the ...
Carly A. Charron, Angelo Kaldis, Shabnam Shamriz, Moussa S. Diarra, Christopher P. Garnham, Rima Menassa   +5 more
wiley   +1 more source

Transient Expression of Coat Protein of Foot and Mouth Disease Virus (FMDV) in Alfalfa (Medicago sativa) by Agroinfiltration [PDF]

Journal of Cell and Molecular Research, 2016
An Agrobacterium-mediated transient gene expression assay was carried out in alfalfa (Medicago sativa) leaves for expression of a chimeric gene encoding a part of capsid protein of Foot and Mouth Disease virus called VP1.
Maziar Habibi-Pirkoohi, Saeid Malekzadeh-Shafaroudi, Hasan Marashi, Saeid Zibaee, Afsaneh Mohkami, Saba NejatizaXeh   +5 more
doaj   +1 more source

A Tobacco Syringe Agroinfiltration-Based Method for a Phytohormone Transporter Activity Assay Using Endogenous Substrates

Frontiers in Plant Science, 2021
Phytohormones are a group of small chemical molecules that play vital roles in plant development, metabolism, and stress responses. Phytohormones often have distinct biosynthesis and signaling perception sites, requiring long- or short-distance ...
Jiangzhe Zhao, Min-No Ju, Jiayun Qian, Mengyuan Zhang, Ting Liu, Ke-Wei Zhang   +5 more
semanticscholar   +1 more source

The ZmRACK1–ZmCDPK7–ZmAPX1 module regulates plant antiviral immunity

Journal of Integrative Plant Biology, EarlyView.
In maize, ZmRACK1 acts as a scaffold protein that bridges the cyclin‐dependent kinase ZmCDPK7 and the ascorbate peroxidase ZmAPX1, promoting ZmAPX1 activity to mitigate MCMV infection. Upon viral infection, P31 obstructs formation of the ZmRACK1‐ZmCDPK7‐ZmAPX1 complex, inhibiting ZmCDPK7‐mediated ZmAPX1 activity and promoting reactive oxygen species ...
Yuyang Zhang, Niannian Li, Qiongqiong Wang, Yingchao Sun, Yajuan Shi, Lianwei Yu, Xiaoyu Han, Xiaoxi Feng, Zaifeng Fan, Linlin Chen, Honglian Li, Xue Yang, Hongxia Yuan, Yan Shi   +13 more
wiley   +1 more source

Transient Gene Expression: an Approach for Recombinant Vaccine Production [PDF]

Journal of Medical Microbiology and Infectious Diseases, 2021
The production of recombinant vaccines in green plants is an attractive and promising topic in genetic engineering. However, the stable transformation of green plants is a time-consuming, costly, and labor-intensive practice.
Maziar Habibi-Pirkoohi, Amir Ghaffar Shahriari, Mohamad Hamed Ghodoum Parizipour   +2 more
doaj   +1 more source

Establishment and optimization of a hemp (Cannabis sativa L.) agroinfiltration system for gene expression and silencing studies

Scientific Reports, 2020
Industrial hemp (Cannabis sativa L.) is a high-yielding annual crop primarily grown for fiber, seeds, and oil. Due to the phytochemical composition of hemp, there has been an increased interest in the market for nutraceuticals and dietary supplements for
M. Deguchi, Daniel Bogush, Hannah Weeden, Zachary Spuhler, S. Potlakayala, T. Kondo, Zhan-Yuan J. Zhang, S. Rudrabhatla   +7 more
semanticscholar   +1 more source