Agrobacterium-mediated barley transformation. [PDF]
, 2008 More than ten years have passed since the first successful Agrobacterium-mediated barley transformation experiment, however it is still quite challenging to establish a stably functioning agroinfiltration protocol.
Csóti, László +2 more
core
Identification and characterization of regulatory proteins involved in anthocyanin biosynthesis in Fragaria vesca and Rubus idaeus [PDF]
, 2019 Primary metabolites like carbohydrates, lipids, proteins, and nucleic acids, work as key components to sustain the plant. Besides those compounds, a wide array of so called ‘secondary metabolites’ are produced by the plant, which have been given much ...
Herrera Valderrama, Andrea Lorena
core +1 more source
Improving transient protein expression in agroinfiltrated Nicotiana benthamiana
New PhytologistSummaryAgroinfiltration of Nicotiana benthamiana is routinely used in plant science and molecular pharming to transiently express proteins of interest. Here, we discuss four phenomena that should be avoided to improve transient expression. Immune responses can be avoided by depleting immune receptors and employing pathogen‐derived effectors; transcript
Beritza, K +2 more
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Agroinfiltration as an Effective and Scalable Strategy of Gene Delivery for Production of Pharmaceutical Proteins [PDF]
Advanced Techniques in Biology & Medicine, 2013 Current human biologics are most commonly produced by mammalian cell culture-based fermentation technologies. However, its limited scalability and high cost prevent this platform from meeting the ever increasing global demand. Plants offer a novel alternative system for the production of pharmaceutical proteins that is more scalable, cost-effective ...
Chen, Qiang +5 more
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The FEBS Journal, EarlyView.This study investigates the modularity of encapsulin—a protein nanoparticle—by simultaneously inserting up to four distinct Salmonella epitopes within the encapsulin subunit. The encapsulin variants were transiently expressed in plants where they assembled into nanoparticles; however, their accumulation and stability varied depending on the ...
Carly A. Charron +5 more
wiley +1 more source
The ZmRACK1–ZmCDPK7–ZmAPX1 module regulates plant antiviral immunity
Journal of Integrative Plant Biology, EarlyView.In maize, ZmRACK1 acts as a scaffold protein that bridges the cyclin‐dependent kinase ZmCDPK7 and the ascorbate peroxidase ZmAPX1, promoting ZmAPX1 activity to mitigate MCMV infection. Upon viral infection, P31 obstructs formation of the ZmRACK1‐ZmCDPK7‐ZmAPX1 complex, inhibiting ZmCDPK7‐mediated ZmAPX1 activity and promoting reactive oxygen species ...
Yuyang Zhang +13 more
wiley +1 more source
Establishment of an Agrobacterium-mediated Inoculation System for Cucumber Green Mottle Mosaic Virus
The Plant Pathology Journal, 2015 The infectious full-length cDNA clones of Cucumber green mottle mosaic virus (CGMMV) isolates KW and KOM, which were isolated from watermelon and oriental melon, respectively, were constructed under the control of the cauliflower mosaic virus 35S ...
Minji Kang +4 more
doaj +1 more source
Production, characterization, and antigen specificity of recombinant 62-71-3, a candidate monoclonal antibody for rabies prophylaxis in humans [PDF]
, 2013 Rabies kills many people throughout the developing world every year. The murine monoclonal antibody (mAb) 62-71-3 was recently identified for its potential application in rabies postexposure prophylaxis (PEP).
Anthony R. Fooks +42 more
core +1 more source
Balancing Selection at the Tomato RCR3 Guardee Gene Family Maintains Variation in Strength of Pathogen Defense [PDF]
, 2012 Coevolution between hosts and pathogens is thought to occur between interacting molecules of both species. This results in the maintenance of genetic diversity at pathogen antigens (or so-called effectors) and host resistance genes such as the major ...
Hoerger, A. +5 more
core +4 more sources
Agroinfiltration-based efficient transient protein expression in leguminous plants
Plant Biotechnology, 2019 Transient protein expression is an effective tool to rapidly unravel novel gene functions, such as transcriptional activity of promoters and sub-cellular localization of proteins. However, transient expression is not applicable to some species and varieties because of insufficient expression levels.
Takuya, Suzaki +4 more
openaire +3 more sources