Results 81 to 90 of about 7,887 (237)
Potyvirus turnip mosaic virus induces neutral lipid accumulation and lipid droplet biogenesis in infected leaves for its own benefit. See also the Commentary on this article by May & Nagy, 252: 525–527. Summary Lipid droplets (LDs), which are dedicated to storing neutral lipids (NLs), are dynamic organelles involved in numerous other functions ...
Léna Jambou +16 more
wiley +1 more source
ABSTRACT Transient expression of exogenous protein in Nicotiana benthamiana leaves via agroinfiltration offers a rapid and efficient platform for functional gene discovery and heterologous production of valuable eukaryotic proteins and metabolites. Though light quality is an important factor for plant photomorphogenesis, its impact on the efficiency of
Yating Zhang +6 more
wiley +1 more source
The Putative Effector Protein AlPE1 From Ascochyta lentis Causes Necrosis in Lentil and Pea
ABSTRACT Ascochyta blight of lentil is caused by the hemibiotrophic fungal pathogen species Ascochyta lentis. Recent advances in whole genome sequencing have led to the availability of many fungal genome assemblies, enabling comparison of isolates that can lead to the identification of virulence genes or effectors.
Robert C. Lee +4 more
wiley +1 more source
Agroinfiltration of Strawberry Fruit — A Powerful Transient Expression System for Gene Validation [PDF]
Agrobacterium-infiltration (agroinfiltration) transient expression systems facilitate rapid primary characterization of candidate gene function, protein localization, protein interacting partners, or other information from a delivered construct.
Carvalho, Raquel F. +3 more
core +1 more source
Agroinfiltration as an Effective and Scalable Strategy of Gene Delivery for Production of Pharmaceutical Proteins [PDF]
Current human biologics are most commonly produced by mammalian cell culture-based fermentation technologies. However, its limited scalability and high cost prevent this platform from meeting the ever increasing global demand. Plants offer a novel alternative system for the production of pharmaceutical proteins that is more scalable, cost-effective ...
Chen, Qiang +5 more
openaire +2 more sources
Turnip Mosaic Virus‐Based gRNA Delivery System for Plant Genome Editing
ABSTRACT Plant virus‐based gRNA delivery systems offer a rapid alternative to stable transformation for CRISPR‐mediated genome editing, but potyvirus‐based platforms in Cas9‐expressing plants are still underexplored. Here, we developed a turnip mosaic virus (TuMV)‐based system for gRNA delivery in Cas9‐expressing Nicotiana benthamiana and tested ...
Ekkachai Khwanbua +3 more
wiley +1 more source
The RHO1‐TIP1;1 interaction affects the compartmentalization of reactive oxygen species (ROS) in cytoplasm and vacuoles, promotes the accumulation of ROS in cytoplasm, leading to different responses of plant cells to pathogens with different lifestyles.
Bowen Yuan +10 more
wiley +1 more source
Specific post-transcriptional gene silencing (PTGS) of target genes can be induced in a variety of organisms by providing homologous double-stranded RNA (dsRNA) molecules. In plants, PTGS is part of a defense mechanism against virus infection.
F. Tenllado +5 more
doaj +1 more source
EC19, a secreted ribonuclease T1 effector from Fusarium oxysporum f. sp. lycopersici (Fol), is required for tomato wilt virulence. EC19‐overexpressing tomato lines display increased susceptibility to Fol and altered transcriptional changes associated with plant hormone signal transduction, plant–pathogen interaction, and phenylpropanoid biosynthesis ...
Min Li +7 more
wiley +1 more source
Plant functional proteomics research is increasingly dependent upon vectors that facilitate high-throughput gene cloning and expression of fusions to autofluorescent proteins.
Romit Chakrabarty +7 more
doaj +1 more source

