Results 101 to 110 of about 7,887 (237)

Glycoform Modification of Secreted Recombinant Glycoproteins through Kifunensine Addition during Transient Vacuum Agroinfiltration [PDF]

, 2018
Kifunensine, a potent and selective inhibitor of class I α-mannosidases, prevents α-mannosidases I from trimming mannose residues on glycoproteins, thus resulting in oligomannose-type glycans.
Qiongyu Li, Somen Nandi, Carlito Lebrilla, Lebrilla, Carlito B, McDonald, Karen A, Nandi, Somen, Muchena Kailemia, Yongao Xiong, Karen McDonald, Li, Qiongyu, Xiong, Yongao, Kailemia, Muchena J   +11 more
core   +1 more source

Establishment of an Agrobacterium-mediated Inoculation System for Cucumber Green Mottle Mosaic Virus

The Plant Pathology Journal, 2015
The infectious full-length cDNA clones of Cucumber green mottle mosaic virus (CGMMV) isolates KW and KOM, which were isolated from watermelon and oriental melon, respectively, were constructed under the control of the cauliflower mosaic virus 35S ...
Minji Kang, Jang-Kyun Seo, Dami Song, Hong-Soo Choi, Kook-Hyung Kim   +4 more
doaj   +1 more source

Optimizing Agroinfiltration for Enhanced Transient Expression of Green Fluorescent Protein in Nicotiana tabacum

Vegetalika
Agroinfiltration is a genetic transformation method that was developed to transiently express genes in plant cells for the rapid production of plant-based recombinant proteins.
W. D. Sawitri
semanticscholar   +1 more source

Agroinfiltration in Z. zerumbet leaves.

, 2015
Fig 3a. Control leaf of infiltrated Z. zerumbet. Agroinfiltration was carried out by mixing equal amounts of the three Agrobacterium (GV3103) cultures harboring pCaBS-α, pCaBS-β and pCa-γb: 00.The cultures were infiltrated into 2–4 leaves of 3-month Z ...
Manjula Sakuntala (731214), Lokesh Deb (400828), Chidambareswaren Mahadevan (731212), Abdul Jaleel (731213), George Thomas (80785)   +4 more
core   +1 more source

pH gradient mitigation in the leaf cell secretory pathway attenuates the defense response of Nicotiana benthamiana to agroinfiltration

, 2020
Partial neutralization of the Golgi lumen pH by the ectopic expression of influenza virus M2 proton channel is useful to stabilize acid-labile recombinant proteins in plant cells, but the impact of pH gradient mitigation on host cellular functions has ...
Lavoie, Pierre-Olivier, Jutras, Philippe V, Sainsbury, Frank, Tardif, Rachel, Michaud, Dominique, Goulet, Marie-Claire, D'aoust, Marc-André, Hamel, Louis-Philippe, D’Aoust, Marc-André, Jutras, Philippe V.   +9 more
core   +1 more source

Vacuum and Co-cultivation Agroinfiltration of (Germinated) Seeds Results in Tobacco Rattle Virus (TRV) Mediated Whole-Plant Virus-Induced Gene Silencing (VIGS) in Wheat and Maize

Frontiers in Plant Science, 2017
Tobacco rattle virus (TRV)-mediated virus-induced gene silencing (VIGS) has been frequently used in dicots. Here we show that it can also be used in monocots, by presenting a system involving use of a novel infiltration solution (containing ...
Ju Zhang, Deshui Yu, Yi Zhang, Kun Liu, Kedong Xu, Fu-Li Zhang, Jian Wang, Guangxuan Tan, Xianhui Nie, Q. Ji, Lu Zhao, Cheng-Wei Li   +11 more
semanticscholar   +1 more source

RD21 characteristics and expression by agroinfiltration.

, 2012
A, Maturation of RD21. RD21 is encoded as a preproprotease with a signal peptide (SP) for entering the secretion pathway and a prodomain (pro) that keeps the protease inactive until it is removed.
Mohammed Shabab (179769), Renier A. L. van der Hoorn (150148), Christian Gu (179767), Melanie Niemer (179779), Pieter J. Wolters (179773), Richard Strasser (179771), Farnusch Kaschani (179782), Lukas Mach (179785), Takayuki Shindo (179777)   +8 more
core   +1 more source

Transcriptome analysis of Phytophthora cactorum infecting strawberry identified RXLR effectors that induce cell death when transiently expressed in Nicotiana benthamiana

Frontiers in Plant Science
Phytophthora cactorum is a plant pathogenic oomycete that causes crown rot in strawberry leading to significant economic losses every year. To invade the host, P.
Bikal Ghimire, Anupam Gogoi, Anupam Gogoi, Mandeep Poudel, Arne Stensvand, Arne Stensvand, May Bente Brurberg, May Bente Brurberg   +7 more
doaj   +1 more source

The pathogenic effector PHYL1JWB interacts with ZjCURT1A to mediate jujube witches' broom‐induced photosynthesis impairment of jujube trees

The Plant Journal, Volume 127, Issue 5, September 2026.
SUMMARY Photosynthesis impairment and the accompanying leaf yellowing represent a prominent and physiologically critical symptom of phytoplasma infection. However, the underlying molecular mechanisms remain poorly understood. In this study, we identified the Jujube Witches' Broom pathogenic effector PHYL1JWB, which was previously reported to induce ...
Fangfang Huang, Changfeng Ai, Chong Yang, Jie Feng, Meng Yang, Li Dai, Chaoling Xue, Hongtai Li, Jiaxue Cui, Xiangjie Qu, Jin Zhao, Mengjun Liu, Xuan Zhao, Zhiguo Liu   +13 more
wiley   +1 more source

A double agroinfiltration procedure to test candidate genes associated with potato late blight resistance mediated by the RB gene.

, 2013
All the pictures were taken at 10 days post infiltration and bars represent 2 cm. (A) Infiltration with Agrobacterium carrying pGR106-IpiO1 and HR response was observed around the infiltrated site.
Muthusubramanian Venkateshwaran (372401), Lei Wu (202933), Pudota B. Bhaskar (372400), Jean-Michel Ané (372402), Jiming Jiang (85639)   +4 more
core   +1 more source