Results 111 to 120 of about 8,861 (240)

A Modified Cas9 Scaffold Allows Extension of the Virus‐Induced Gene Editing Technology to the Large Potyvirus Genus

open access: yesPlant Biotechnology Journal, EarlyView.
ABSTRACT Plant viruses are recognized as rapid and effective vectors to deliver CRISPR‐Cas reaction components into plants, a strategy termed virus‐induced gene editing (VIGE). However, VIGE is limited by the host range of the viral vectors. Development of new viral vectors to target a broad range of plant species will potentially enable the delivery ...
Fernando Merwaiss   +3 more
wiley   +1 more source

GoldenBraid-compatible infectious clone of apple latent spherical virus (ALSV) and its use for virus-induced gene silencing

open access: yesBiologia Plantarum
Virus-induced gene silencing (VIGS) is a technological process in which the expression of a plant target gene is down-regulated by inoculating a plant with a recombinant virus-based vector carrying part of the coding sequence of the target gene ...
Hana HOFFMEISTEROVÁ   +4 more
doaj   +1 more source

Genome analysis and avirulence gene cloning using a high-density RADseq linkage map of the flax rust fungus, Melampsora lini [PDF]

open access: yes, 2016
Agroinfiltration of avirulence gene constructs. The response of flax cultivars and near-isogenic lines to expression of avirulence gene candidates (AvrM14-A, AvrM14-B and AvrL2-A) using Agrobacterium tumefaciens-mediated transient transformation.
Adnane Nemri   +10 more
core   +11 more sources

Gene Editing of Nicotiana benthamiana Architecture for Space‐Efficient Production of Recombinant Proteins in Closed Environments

open access: yesPlant Biotechnology Journal, EarlyView.
ABSTRACT Indoor vertical farming (VF) offers practical advantages for the cultivation of plant protein bio‐factories including plant uniformity, product consistency, water/nutrient recycling and production cycles on a year‐round basis. Much progress has been achieved toward the development of innovative systems for artificial lighting, automated ...
Béatrice Giroux   +5 more
wiley   +1 more source

CsCBDAS2-Driven Enhancement of Cannabinoid Biosynthetic Genes Using a High-Efficiency Transient Transformation System in Cannabis sativa ‘Cheungsam’

open access: yesPlants
Cannabis sativa produces pharmacologically valuable cannabinoids. In this study, we developed and optimized a transient transformation system using Cannabis sativa ‘Cheungsam’ to facilitate gene functional analysis.
Sang-Cheol Baek   +6 more
doaj   +1 more source

Expression Levels of avrBs3-Like Genes Affect Recognition Specificity in Tomato Bs4- But Not in Pepper Bs3-Mediated Perception

open access: yesMolecular Plant-Microbe Interactions, 2005
The tomato Bs4 disease resistance gene mediates recognition of avrBs4-expressing strains of the bacterial spot pathogen Xanthomonas campestris pv. vesicatoria to give a hypersensitive response (HR).
Sebastian Schornack   +3 more
doaj   +1 more source

Multi‐Omics Analyses Reveal the Red and Far‐Red Light Combination Enhancing Heterologous Protein and Metabolite Production in Nicotiana benthamiana

open access: yesPlant, Cell &Environment, EarlyView.
ABSTRACT Transient expression of exogenous protein in Nicotiana benthamiana leaves via agroinfiltration offers a rapid and efficient platform for functional gene discovery and heterologous production of valuable eukaryotic proteins and metabolites. Though light quality is an important factor for plant photomorphogenesis, its impact on the efficiency of
Yating Zhang   +6 more
wiley   +1 more source

Development of the HTRF assay to evaluate the auxin‐induced binding between TIR1 and IAA7

open access: yesNew Phytologist, Volume 250, Issue 5, Page 3475-3485, June 2026.
Summary Auxin plays diverse roles in plant growth and development, including sensing environmental changes. Quantifying the interaction between auxin coreceptors provides the molecular basis for cells to sense and adapt to environmental cues. Although several assays are available, a more high‐throughput method is necessary to efficiently evaluate the ...
Jekson Robertlee, Shinya Hagihara
wiley   +1 more source

Exploration of the Interactions Between Xanthomonas citri subsp. citri–Agrobacterium–Citrus to Improve Agrobacterium-Mediated Transient Expression in Plants

open access: yesMolecular Plant-Microbe Interactions
Agrobacterium-mediated transient expression (AMTE) is an important tool in plant genetics studies and biotechnology. AMTE remains problematic in citrus and many plant species.
Tirtha Lamichhane   +3 more
doaj   +1 more source

An antiviral jacalin‐like lectin gene contributes to nonhost resistance and host determination of potexviruses among Brassicaceae

open access: yesNew Phytologist, Volume 250, Issue 5, Page 3283-3298, June 2026.
Summary Plant resistance influences the host range of pathogens; however, the mechanism driving this influence remains poorly understood. Brassicaceae species are not natural hosts of potexviruses, for reasons that have not yet been determined. This study investigated the molecular basis of nonhost resistance (NHR) to potexviruses in the Brassicaceae ...
Takumi Suzuki   +11 more
wiley   +1 more source

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