Results 111 to 120 of about 7,887 (237)

Leaf-Encapsulated Vaccines: Agroinfiltration and Transient Expression of the Antigen Staphylococcal Endotoxin B in Radish Leaves [PDF]

, 2018
Transgene introgression is a major concern associated with transgenic plant-based vaccines. Agroinfiltration can be used to selectively transform nonreproductive organs and avoid introgression.
Yanhan Wang, Christopher W. Simmons, Robert G. Ulrich, Richard L. Gallo, Wang, Yanhan, Huang, Chun-Ming, Liu, Pei-Feng, Ulrich, Robert G, Simmons, Christopher W, Pei-Feng Liu, Chun-Ming Huang, VanderGheynst, Jean S, Jean S. VanderGheynst, Gallo, Richard L   +13 more
core   +1 more source

Hairpin RNA-mediated gene silencing of NRCX enhances NRC2- and NRC3-dependent hypersensitive cell death.

, 2023
(A) Hypersensitive cell death phenotypes after co-expressing different NRC-S and AVR combinations with hpRNA:GUS (control) or hpRNA:NRCX by agroinfiltration.
Chih-hang Wu (7056494), Hiroaki Adachi (133026), Sophien Kamoun (6132), Jules Claeys (14474854), Toshiyuki Sakai (309632), Tolga O. Bozkurt (14020459), Hsuan Pai (13844742), AmirAli Toghani (14474851), Cian Duggan (7056497), Adeline Harant (4783905), Kodai Honda (14474848)   +10 more
core   +1 more source

The regulatory frameworks surrounding CRISPR‐edited papaya and their impact on international commerce

Journal of the Science of Food and Agriculture, Volume 106, Issue 11, Page 6262-6270, 30 August 2026.
Abstract The papaya tree (Carica papaya L.), native to the Americas, is cultivated in tropical regions and holds substantial economic importance, with an estimated export volume of 365 000 t in 2023. However, diseases caused by viruses, fungi, bacteria, and nematodes can lead to severe losses.
Luíza Favaratto, Mirielson L da Silva, David S Buss, Oeber F Quadros, Raul Tapia‐Tussell, José A Ventura, Antonio Alberto R Fernandes, Patricia M B Fernandes   +7 more
wiley   +1 more source

Comparison of transient transformation efficiencies in different plant materials by using agroinfiltration.

, 2014
Note: three samples per material were investigated for ten 2 mm2 areas of agroinfiltrated epidermal cells. Each efficiency value represents the mean of transformation efficiencies of thirty replicates of 2 mm2 epidermal areas from three samples (the ...
Yi Zhang (9093), Kedong Xu (506662), Manman Wu (506663), Kun Liu (148201), Fuli Zhang (506665), Chengwei Li (181253), Yunxia Chang (506664), Ruiyue Wang (506667), Xiaohui Huang (458909), Yan Wang (15435), Liming Yi (506666), Ju Zhang (281062), Tingting Li (10553), Guangxuan Tan (506668)   +13 more
core   +1 more source

Phytophthora infestans effector AVR3a is essential for virulence and manipulates plant immunity by stabilizing host E3 ligase CMPG1 [PDF]

, 2010
Fungal and oomycete plant pathogens translocate effector proteins into host cells to establish infection. However, virulence targets and modes of action of their effectors are unknown.
Hein, I., Dixelius, Christina, Hein, I, Hein, Ingo, Bryan, G, Dixelius, C., Kamoun, S., Gilroy, EM, Bos, JIB, Sadanandom, A, Boevink, PC, Gilroy, Eleanor M., Armstrong, MR, Bos, J. I. B., Taylor, RM, Bos, Jorunn I. B., Zhendong, T., Sadanandom, A., Boevink, Petra C., Dixelius, C, Engelhardt, Stefan, Bryan, Glenn, Birch, PRJ, Armstrong, M. R., Engelhardt, S, Hein, Ingo; id_orcid, Harrower, Brian, Kamoun, Sophien, Whisson, Stephen C., Vetukuri, R. R., Birch, Paul R. J.; id_orcid, Bryan, G., Vetukuri, RR, Engelhardt, S., Whisson, S. C., Tian, ZD, Whisson, SC, Boevink, P. C., Bryan, Glenn J., Bos, Jorunn, Gilroy, E. M., Sadanandom, Ari, Birch, Paul R. J., Harrower, B., Harrower, B, Vetukuri, Ramesh R., Birch, P. R. J., Taylor, R. M., Armstrong, Miles R., Taylor, Rosalind M., Zhendong, Tian, Kamoun, S, Tian, Zhendong, Sveriges lantbruksuniversitet., Ramesh, Vetukuri,, Dixelius, Christina,   +55 more
core   +1 more source

Hairpin RNA-mediated gene silencing of NRCX does not cause cell death in Nicotiana benthamiana leaves

, 2023
(A) Macroscopic cell death phenotype after expressing hpRNA:GUS, hpRNA:NRCX or Pto/AvrPto by agroinfiltration. Photograph was taken at 5 days after the agroinfiltration.
Chih-hang Wu (7056494), Hiroaki Adachi (133026), Sophien Kamoun (6132), Jules Claeys (14474854), Toshiyuki Sakai (309632), Tolga O. Bozkurt (14020459), Hsuan Pai (13844742), AmirAli Toghani (14474851), Cian Duggan (7056497), Adeline Harant (4783905), Kodai Honda (14474848)   +10 more
core   +1 more source

Optimization of Agroinfiltration in Pisum sativum Provides a New Tool for Studying the Salivary Protein Functions in the Pea Aphid Complex [PDF]

, 2016
Aphids are piercing-sucking insect pests and feed on phloem sap. During feeding, aphids inject a battery of salivary proteins into host plant. Some of these proteins function like effectors of microbial pathogens and influence the outcome of plant-aphid ...
Yoann Aigu, Boulain, H., Akiko Sugio, GUY, Endrick, Hélène Boulain, Khaoula Chawki, Le Pennec, C., Morliere, Stéphanie, Grit Kunert, Sugio, A., Guy, E., Le Pennec, Charlotte, Kristina schaedel, Kunert, Grit, Chawki, K., Kunert, G., Endrick Guy, Charlotte Le Pennec, Schädel, K., Morlière, S., Schädel, Kristina, Chawki, Khaoula, Aigu, Yoann, Stéphanie Morlière, Simon, Jean-Christophe, Simon, J., Kristina Schädel, Sugio, Akiko, Jean-Christophe Simon, BOULAIN, Hélène, Aigu, Y.   +30 more
core   +1 more source

Assessing Carnivorous Plants for the Production of Recombinant Proteins

Frontiers in Plant Science, 2019
The recovery of recombinant proteins from plant tissues is an expensive and time-consuming process involving plant harvesting, tissue extraction, and subsequent protein purification.
Sissi Miguel, Estelle Nisse, Flore Biteau, Sandy Rottloff, Benoit Mignard, Eric Gontier, Alain Hehn, Frédéric Bourgaud   +7 more
doaj   +1 more source

The transcriptome, extracellular proteome and active secretome of agroinfiltratedNicotiana benthamianauncover a large, diverse protease repertoire [PDF]

, 2017
Infiltration of disarmed Agrobacterium tumefaciens into leaves of Nicotiana benthamiana (agroinfiltration) facilitates quick and safe production of antibodies, vaccines, enzymes and metabolites for industrial use (molecular farming).
Grosse-Holz, Friederike M., Kaiser, M, Kelly, S, Grosse-Holz, FM, Kaschani, F, Renier A.L. van der Hoorn, Farnusch Kaschani, Steven Kelly, Friederike Grosse-Holz, Kelly, Steven, Blaskowski, S, van der Hoorn, RAL, Markus Kaiser, van der Hoorn, Renier A.L., Svenja Blaskowski   +14 more
core   +1 more source

Agroinfiltration leaf patch assays of GFP gene constructs containing 3′ hairpin trailer.

, 2014
(A) Schematic representation of GFP constructs containing the different 3′ trailer sequences analyzed. The noHP sequence consists of a partial N gene sequence in antisense polarity. (B) Transient GFP expression after agroinfiltration of GFP constructs in
Dick Lohuis (636618), Marcio Hedil (636616), Richard Kormelink (182162), Afshin Hassani-Mehraban (636617)   +3 more
core   +1 more source