Results 71 to 80 of about 7,887 (237)

The concept of an agroinfiltration kit for recombinant protein production for educational and commercial use—A journey through a forest of regulatory and legal implications

Frontiers in Bioengineering and Biotechnology, 2022
Recombinant expression using Agrobacterium-mediated transient transformation (ATT) of plants has developed into a robust and versatile method to rapidly produce proteins. The capability of plants to efficiently synthesize even homo- and hetero-multimeric
H. Spiegel, Greta Nölke, Harry Thangaraj, S. Schillberg   +3 more
semanticscholar   +1 more source

An efficient system to detect protein ubiquitination by agroinfiltration inNicotiana benthamiana [PDF]

The Plant Journal, 2010
The ubiquitination proteasome pathway has been demonstrated to regulate all plant developmental and signaling processes. E3 ligase/substrate-specific interactions and ubiquitination play important roles in this pathway. However, due to technical limitations only a few instances of E3 ligase-substrate binding and protein ubiquitination in plants have ...
Lijing, Liu, Yiyue, Zhang, Sanyuan, Tang, Qingzhen, Zhao, Zhonghui, Zhang, Huawei, Zhang, Li, Dong, Huishan, Guo, Qi, Xie   +8 more
openaire   +2 more sources

Harnessing Bulk‐Segregant Mapping to Identify Trait‐Associated Genes in the Allopolyploid Model Plant Nicotiana benthamiana

Plant Biotechnology Journal, EarlyView.
ABSTRACT Forward genetics has been instrumental in identifying genes underlying desirable traits, yet its application to polyploid plants, many of which are key agricultural crops, remains challenging due to their genomic complexity. Therefore, we developed BenthMap, a bulk segregant analysis platform for high‐throughput trait mapping and gene ...
Zuba Ahmed, Jiyuan An, Satomi Hayashi, Julia Bally, Chris Winefield, Peter M. Waterhouse   +5 more
wiley   +1 more source

Knocking Out Two Polyphenol Oxidase Genes Significantly Improves Recombinant Protein Purification in Nicotiana benthamiana

Plant Biotechnology Journal, EarlyView.
ABSTRACT Efficient purification remains a key technical challenge affecting the recovery efficiency of recombinant proteins in plant‐based systems. Complex metabolites, particularly polyphenols, often cause recombinant protein aggregation during purification.
Hai‐Ping Diao, Hui‐Xin Meng, Xue‐Jiao Xu, Zeng‐Lin Zhang, Jian‐Feng Zhang, Yong‐Feng Guo, Inhwan Hwang, Shi‐Jian Song   +7 more
wiley   +1 more source

Agrosuppression: A Bioassay for the Hypersensitive Response Suited to High-Throughput Screening

Molecular Plant-Microbe Interactions, 2003
We describe a novel method, agrosuppression, that addresses the need for an assay of the hypersensitive response (HR) in intact plants that is rapid and adapted to high-throughput functional screening of plant and pathogen genes.
Sophien Kamoun, Walid Hamada, Edgar Huitema   +2 more
doaj   +1 more source

Screening of Secreted Proteins of Sporisorium reilianum f. sp. zeae for Cell Death Suppression in Nicotiana benthamiana

Frontiers in Plant Science, 2020
Sporisorium reilianum f. sp. zeae (SRZ) is a biotrophic fungus causing head smut in maize. Maize infection with SRZ leads to very little cell death suggesting the presence of cell-death suppressinpg effectors.
Deiziane Dutra, Nisha Agrawal, Nisha Agrawal, Hassan Ghareeb, Hassan Ghareeb, Jan Schirawski, Jan Schirawski, Jan Schirawski   +7 more
doaj   +1 more source

Gene Editing of Nicotiana benthamiana Architecture for Space‐Efficient Production of Recombinant Proteins in Closed Environments

Plant Biotechnology Journal, EarlyView.
ABSTRACT Indoor vertical farming (VF) offers practical advantages for the cultivation of plant protein bio‐factories including plant uniformity, product consistency, water/nutrient recycling and production cycles on a year‐round basis. Much progress has been achieved toward the development of innovative systems for artificial lighting, automated ...
Béatrice Giroux, Kristina LeBreux, Louis Feyzeau, Marie‐Claire Goulet, Charles Goulet, Dominique Michaud   +5 more
wiley   +1 more source

Production of Genistein in Amaranthus tricolor var. tristis and Spinacia oleracea by Expression of Glycine max Isoflavone Synthase

Plants, 2021
Isoflavonoids, the diverse group of secondary metabolites derived from the phenylpropanoid pathway, are distributed predominantly in leguminous plants.
Ashwini Malla, Balamurugan Shanmugaraj, Ashutosh Sharma, Sathishkumar Ramalingam   +3 more
doaj   +1 more source

A Fusion Between Common Bean Phaseolin and the N‐Terminal Domain of Maize 16 kDa Gamma Zein: Clues for Designing Nutritionally Improved Seed Storage Proteins

Plant Biotechnology Journal, EarlyView.
ABSTRACT Increasing the nutritional value of seed storage proteins (SSPs) has been largely hindered by limited knowledge of how the features of the various SSP domains determine high accumulation in protein storage vacuoles (PSV) or as endoplasmic reticulum (ER)‐located insoluble protein bodies (PB).
Emma Costa, Lucrezia Luciani, Mahsa Taghipour Rahimi, Alessandro Vitale, Emanuela Pedrazzini   +4 more
wiley   +1 more source

RNA interference (RNAi) construct preparation and agroinfiltration.

, 2013
(A) Diagrammatic representation of RNAi silencing constructs prepared by using pFGC1008 vector backbone. The partial fragments of genes (sense and antisense) were cloned in inverted repeat orientation surrounding the GUS intron.
Praveen Guleria (453941), Sudesh Kumar Yadav (149395)   +1 more
core   +1 more source