Transient Expression of Tetrameric Recombinant Human Butyrylcholinesterase in Nicotiana benthamiana. [PDF]
, 2016 To optimize the expression, extraction and purification of plant-derived tetrameric recombinant human butyrylcholinesterase (prBChE), we describe the development and use of plant viral amplicon-based gene expression system; Tobacco Mosaic Virus (TMV) RNA-
Alkanaimsh, Salem +13 more
core +1 more source
Agroinfiltration and PVX agroinfection in potato and Nicotiana benthamiana.
Journal of visualized experiments : JoVE, 2014 Agroinfiltration and PVX agroinfection are two efficient transient expression assays for functional analysis of candidate genes in plants. The most commonly used agent for agroinfiltration is Agrobacterium tumefaciens, a pathogen of many dicot plant species. This implies that agroinfiltration can be applied to many plant species.
Du, J. +2 more
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DNA Methylation Suppression by Bhendi Yellow Vein Mosaic Virus
Epigenomes, 2018 Bhendi yellow vein mosaic virus (BYVMV) belongs to the monopartite begomovirus associated with the β satellite. As a single-stranded DNA (ssDNA) virus, it should be amenable to transcriptional and post-transcriptional gene silencing (TGS and PTGS ...
Kandhalu Sagadevan Dinesh Babu +9 more
doaj +1 more source
Improvement of the transient expression system for production of recombinant proteins in plants [PDF]
, 2018 An efficient and high yielding expression system is required to produce recombinant proteins. Furthermore, the transient expression system can be used to identify the localization of proteins in plant cells.
Ezura Hiroshi +11 more
core +2 more sources
Within leaf variation is the largest source of variation in agroinfiltration of Nicotiana benthamiana [PDF]
Plant Methods, 2015 Transient gene expression utilizing syringe agroinfiltration offers a simple and efficient technique for different transgenic applications. Leaves of Nicotiana benthamiana show reliable and high transformation efficiency, but in quantitative assays also a certain degree of variation.
Hany Bashandy +2 more
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LbCas12a mediated suppression of Cotton leaf curl Multan virus
Frontiers in Plant Science, 2023 Begomoviruses are contagious and severely affect commercially important fiber and food crops. Cotton leaf curl Multan virus (CLCuMuV) is one of the most dominant specie of Begomovirus and a major constraint on cotton yield in Pakistan.
Sidra Ashraf +8 more
doaj +1 more source
High-level HIV-1 Nef transient expression in using the P19 gene silencing suppressor protein of Artichoke Mottled Crinckle Virus [PDF]
, 2009 Background In recent years, different HIV antigens have been successfully expressed in plants by either stable transformation or transient expression systems.
Raffaele Lombardi +9 more
core +2 more sources
Metabolites, 2023 Crocins are glycosylated apocarotenoids with strong coloring power and anti-oxidant, anticancer, and neuro-protective properties. We previously dissected the saffron crocin biosynthesis pathway, and demonstrated that the CsCCD2 enzyme, catalyzing the ...
Olivia Costantina Demurtas +7 more
doaj +1 more source
Agromonas: a rapid disease assay for Pseudomonas syringae growth in agroinfiltrated leaves [PDF]
The Plant Journal, 2020 Summary The lengthy process to generate transformed plants is a limitation in current research on the interactions of the model plant pathogen Pseudomonas syringae with plant hosts.
Buscaill, P +5 more
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Advanced Science, EarlyView.ABSTRACT Viruses often hijack host developmental programs to promote infection, but the mechanistic links between reproductive regulation and antiviral immunity remain incompletely understood. Here, we identify a virus‐triggered hierarchical degradation cascade that links antiviral immunity and fertility regulation in rice. We show that the rice grassy
Yuansheng Wu +13 more
wiley +1 more source