Results 21 to 30 of about 7,887 (237)

Agroinfiltration-based efficient transient protein expression in leguminous plants.

open access: yesPlant Biotechnology, 2019
Transient protein expression is an effective tool to rapidly unravel novel gene functions, such as transcriptional activity of promoters and sub-cellular localization of proteins.
Takuya Suzaki   +4 more
semanticscholar   +4 more sources

Transient expression of CCL21 chemokine in chickpea via agroinfiltration [PDF]

open access: yesپژوهش‌های حبوبات ایران, 2019
IntroductionChemokine (C-C motif) ligand 21 (CCL21) has anti-tumor efficacy and used for immunotherapy based on cytokinins against cancer cells. Immune potentiating of CCL21 chemokine via DC and stromal cell-based approaches for effective recruitment and activation of APC and T cells for promotion of antitumor activity in lung cancer was evaluated ...
Maria Beihaghi   +4 more
openaire   +3 more sources

Transient GUS gene expression in cassava (Manihot esculenta Crantz) using Agrobacterium tumefaciens leaf infiltration [PDF]

open access: yesRevista Mvz Cordoba, 2014
Objective. Assess transient gene expression of GUS in cassava (Manihot esculenta Crantz) leaves using Agrobacterium tumefaciens infiltration. Materials and methods. A.
Paula Díaz T.   +2 more
doaj   +3 more sources

NbRD21 protease controls receptor kinase homeostasis in Nicotiana benthamiana. [PDF]

open access: yesNew Phytol
Proteomic analysis revealed that receptor kinases (red) accumulate higher in rd21 knockout Nicotiana benthamiana plants. Summary RD21‐like proteases are papain‐like cysteine proteases with a C‐terminal granulin domain that are abundant and ubiquitous in angiosperms and have often been implicated in immunity.
Godson A   +13 more
europepmc   +2 more sources

Agrobacterium-mediated transient gene expression and silencing: a rapid tool for functional gene assay in potato. [PDF]

open access: yesPLoS ONE, 2009
Potato is the third most important food crop worldwide. However, genetic and genomic research of potato has lagged behind other major crops due to the autopolyploidy and highly heterozygous nature associated with the potato genome.
Pudota B Bhaskar   +4 more
doaj   +1 more source

Manipulating resistance to mungbean yellow mosaic virus in greengram (Vigna radiata L): Through CRISPR/Cas9 mediated editing of the viral genome

open access: yesFrontiers in Sustainable Food Systems, 2022
Clustered regularly interspaced short palindromic repeats (CRISPR) associated protein (CRISPR/Cas9) is an adaptive immune system of bacteria to counter the impending viral pathogen attack.
Ashwini Talakayala   +5 more
doaj   +1 more source

Development of a Novel Assay Based on Plant-Produced Infectious Bursal Disease Virus VP3 for the Differentiation of Infected From Vaccinated Animals

open access: yesFrontiers in Plant Science, 2021
Infectious bursal disease virus is the causative agent of Gumboro disease, a severe infection that affects young chickens and is associated with lymphoid depletion in the bursa of Fabricius.
Alessio Bortolami   +11 more
doaj   +1 more source

A Methodological Advance of Tobacco Rattle Virus-Induced Gene Silencing for Functional Genomics in Plants

open access: yesFrontiers in Plant Science, 2021
As a promising high-throughput reverse genetic tool in plants, virus-induced gene silencing (VIGS) has already begun to fulfill some of this promise in diverse aspects.
Gongyao Shi   +11 more
doaj   +1 more source

Recent Advances of In Vitro Culture for the Application of New Breeding Techniques in Citrus

open access: yesPlants, 2020
Citrus is one of the most important fruit crops in the world. This review will discuss the recent findings related to citrus transformation and regeneration protocols of juvenile and adult explants.
Lara Poles   +5 more
doaj   +1 more source

A robust agroinfiltration method [PDF]

open access: yes, 2023
Stable transformation of soybean (Glycine max) is a markedly slow and laborious process. Thus, a tool that enables rapid evaluation of genetic elements in planta is critical to advance complex research and genetic engineering in soybean.
Trull, Bryce N
core  

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