Results 51 to 60 of about 7,003,490 (250)

Rapid Construction and Application of a Vector for Tobacco Ringspot Virus-Induced McPDS Silencing in Bitter Gourd

Horticulturae
The aim of this study is to facilitate the construction of virus-induced gene silencing vectors and to provide a reference or positive control for gene silencing in bitter gourd.
Lingen Zeng, Hui Zhang, Jinju Guo, Zhijun Deng, Hongbiao Liu, Hu Du, Yujuan Zhong, Changyuan Zhang, Chunpeng Yao   +8 more
doaj   +1 more source

VIGS as a strategy to reverse aphid wing induction by Y‐satellite RNA of cucumber mosaic virus

FEBS Open Bio, 2023
Y‐satellite RNA (Y‐sat) of cucumber mosaic virus upregulates the expression of the aphid ABCG4 gene, which promotes aphid wing formation. We used ABCG4 virus‐induced gene silencing (VIGS) to prevent the wing‐induction mechanism of Y‐sat and thus ...
Hangil Kim, Chikara Masuta
doaj   +1 more source

Virus induced gene silencing (VIGS) for functional anaylsis of wheat genes involved in Zymoseptoria tritici susceptibiblity and resistance [PDF]

, 2015
Virus-induced gene silencing (VIGS) has emerged as a powerful reverse genetic technology in plants supplementary to stable transgenic RNAi and, in certain species, as a viable alternative approach for gene functional analysis. The RNA virus Barley stripe
Rudd, J. J., Kanyuka, K., Lee, W-S., Kanyuka, Kostya, Rudd, Jason   +4 more
core   +1 more source

Partial FAK suppression promotes tumor growth, an effect reversed by macrophage p110δ PI3K inactivation

Molecular Oncology, EarlyView.
Partial inhibition of focal adhesion kinase (FAK) can paradoxically promote tumor growth, rather than simply producing a weaker antitumor effect than that observed with strong FAK suppression. In breast cancer and melanoma models, targeting p110δ PI3K, particularly in macrophages, counteracted these tumor‐promoting effects, highlighting the importance ...
Lydia Xenou, Niki Tzenaki, Anna Tsapara, Maria Tzardi, Evangelia A. Papakonstanti   +4 more
wiley   +1 more source

Cassava geminivirus agroclones for virus-induced gene silencing in cassava leaves and roots

Plant Methods, 2018
Aim We report the construction of a Virus-Induced Gene Silencing (VIGS) vector and an agroinoculation protocol for gene silencing in cassava (Manihot esculenta Crantz) leaves and roots.
Ezequiel Matias Lentz, Joel-Elias Kuon, Adrian Alder, Nathalie Mangel, Ima M. Zainuddin, Emily Jane McCallum, Ravi Bodampalli Anjanappa, Wilhelm Gruissem, Hervé Vanderschuren   +8 more
doaj   +1 more source

NAPRT loss promotes lung tumor initiation and growth through AKT signaling independently of NAD+ biosynthesis

Molecular Oncology, EarlyView.
Loss of NAPRT promotes lung tumor initiation and growth through a noncanonical mechanism, independent of its role in NAD+ biosynthesis. Mechanistically, NAPRT depletion activates the mTORC2‐driven AKT/β‐catenin signaling axis to enhance clonogenic and invasive phenotypes. Furthermore, lung‐specific Naprt deletion significantly increases tumor burden in
Myung Joon Oh, Ji Yun Jang, Yoon Jeon, Gi‐Cheon Kim, Hwanho Lee, Jooyoung Lee, Ju‐Hwa Kim, Hakhyun Kim, Minjee Kim, Ho‐Keun Kwon, Ho Lee, Hyun Seok Kim   +11 more
wiley   +1 more source

Regulation of the lncRNA NEAT1 by p53‐ΔNp63 crosstalk modulates the DNA damage response and therapeutic efficacy in HNSCC

Molecular Oncology, EarlyView.
In head and neck squamous cell carcinoma (HNSCC) p53 and p63 exert opposite roles on the transcription regulation of the lncRNA NEAT1. Under basal conditions, p53 levels are low and p63 represses NEAT1 expression. Upon genotoxic stress, p53 is rapidly induced, displacing p63 from the NEAT1 promoter leading to NEAT1 transcriptional activation and ...
Sara De Domenico, Veronica La Banca, Silvia D'Amico, Stefano Scalera, Sara Nicolai, Angelo Peschiaroli   +5 more
wiley   +1 more source

Modification of Tobacco rattle virus RNA1 to Serve as a VIGS Vector Reveals That the 29K Movement Protein Is an RNA Silencing Suppressor of the Virus

Molecular Plant-Microbe Interactions, 2013
Tobacco rattle virus (TRV) has a bipartite, positive-sense single-stranded RNA genome and is widely used for virus-induced gene silencing (VIGS) in plants.
Xianbao Deng, Jani Kelloniemi, Tuuli Haikonen, Anssi L. Vuorinen, Paula Elomaa, Teemu H. Teeri, Jari P. T. Valkonen   +6 more
doaj   +1 more source

Small RNA pathways in mammalian oocytes

FEBS Open Bio, EarlyView.
Three distinct small RNA pathways operate in mammalian oocytes: RNAi interference (RNAi), the microRNA (miRNA) pathway, and the PIWI‐associated RNA (piRNA) pathway. These pathways use small RNAs to guide sequence‐specific repression and contribute to oocyte biology by targeting genes and mobile elements or appear insignificant since different ...
Petr Svoboda, Josef Pasulka
wiley   +1 more source

Establishment of a TRV-VIGS system in Coix (Coix lacryma-jobi L.) for functional study of host–pathogen interactions

Frontiers in Plant Science
Coix (Coix lacryma-jobi L.) is an important cereal and medicinal crop threatened by smut disease (Ustilago coicis). However, the unavailability of rapid and efficient functional genomics tools has impeded progress in understanding gene function and host ...
Xiaosheng Zhao, Guocui Wang, Fang He, Xiang-Dong Li, Chunli Zhou, Qiuping Liu, Leitao Tan, Qingbei Weng, Qingbei Weng   +8 more
doaj   +1 more source