Results 31 to 40 of about 35,695,125 (238)

Engineering peptides into antibodies—opportunities and strategies for therapeutic innovation

FEBS Letters, EarlyView.
Peptides and antibodies occupy complementary therapeutic niches. Peptides recognize difficult targets in a compact format, while antibodies add specificity, long half‐life, and effector functions. This review examines strategies that merge both modalities—peptide grafting into loops, terminal and Fc fusions, and bioconjugation—highlighting how ...
Jinling Wang, Ruslana Romaniuk, Hristo L. Svilenov   +2 more
wiley   +1 more source

Stabilizing an all-aqueous two-phase system using oil-in-water droplets to mimic double emulsions [PDF]

, 2023
The production of water-in-water emulsion droplets, the coalescence of which is prevented by adding oil-in-water micrometric droplets is reported. Hexadecane (O) and cetyl trimethyl ammonium bromide (CTAB) were added to a W/W emulsion made of dextran ...
J-Paul, Douliez, Douliez, Jean-Paul
core   +1 more source

Emerging experimental and computational methods for studying redox‐regulated structural transitions

FEBS Letters, EarlyView.
Redox reactions can reshape proteins and alter how they behave in cells, with important consequences for health and disease. This review explores emerging experimental and computational approaches for discovering these redox‐sensitive protein switches, revealing their structural effects, and predicting their behavior, opening new opportunities to ...
Tasneem Rass, Dana Reichmann, Gábor Erdős   +2 more
wiley   +1 more source

Single-step partitioning in aqueous two-phase systems

, 2000
Partitioning in aqueous two-phase system (ATPS) provides a rapid and gentle means of separation of soluble as well as particulate biomaterials, e.g. proteins, nucleic acids, cells, viruses, organelles, and membranes.
Hatti-Kaul, Rajni,, Franco, Telma Teixeira, Hatti-Kaul, Rajni   +2 more
core   +1 more source

Malic acid extraction from aqueous solution by using aqueous two-phase system method

Results in Chemistry, 2019
In this investigation malic acid extraction by aqueous two-phase system method (ATPS) was performed. ATPS are involved polymer (PEG 4000 and 8000) and various phosphate salts. Effect of PEG molecular weight, pH, temperature and concentration of salt were
Hadiseh Masoumi, Hamid Ramezanipour Penchah, Hossein Ghanadzadeh Gilani, Tahereh Jangjooye Shaldehi   +3 more
doaj   +1 more source

The Shewanella oneidensis Fic enzyme SoFic targets the switch‐I region of EF‐Tu for AMPylation

FEBS Letters, EarlyView.
Fic enzymes mediate diverse post‐translational modifications across all domains of life, including AMPylation. Prokaryotic EF‐Tu can be AMPylated and deAMPylated by the conserved Fic enzyme SoFic. Structural and biochemical approaches were used to characterize the effect of AMPylation on EF‐Tu, SoFic's enzymatic activities, and the enzyme‐target ...
Svenja Runge, Vivian Pogenberg, Alexander Baumgart, Bente Siebels, Hartmut Schlüter, Michael Hecht‐Bucher, Aymelt Itzen   +6 more
wiley   +1 more source

Aqueous two-phase systems : A general overview

, 2000
Biphasic systems formed by mixing of two polymers or a polymer and a salt in water can be used for separation of cells, membranes, viruses, proteins, nucleic acids, and other biomolecules.
Hatti-Kaul, Rajni,, Hatti-Kaul, Rajni
core   +1 more source

Bioconversion of apigenin-7-O-β-glucoside in aqueous two-phase system [PDF]

Acta Periodica Technologica, 2005
The study is concerned with the conversion of apigenin-7-O-β-glucoside into apigenin in polyethylene glycol 6000 / dextran 20000 aqueous two-phase system by β-glucosidase.
Ilić Sanja M., Đaković Sanja D., Cvejić Jelena H., Antov Mirjana G., Zeković Zoran P.   +4 more
doaj   +1 more source

Simple Purification and Antimicrobial Properties of Bacteriocin-like Inhibitory Substance from Bacillus Species for the Biopreservation of Cheese

Foods, 2023
Bacteriocins may be used as natural preservatives and antibiotic substitutes in various foods. However, the multistep purification process of bacteriocins results in high production costs, which is an obstacle to their commercial use and consumer ...
Jong-Hui Kim, Eun-Seon Lee, Bu-Min Kim, Jun-Sang Ham, Mi-Hwa Oh   +4 more
doaj   +1 more source

Chiral separation of chloroalkanes with the chromatographic column onboard Martian rovers

FEBS Letters, EarlyView.
Computer image of the Rosalind Franklin Rover of ESA's ExoMars mission. ExoMars is scheduled to land on planet Mars in Oxia Planum in 2029. This area represents an interesting spot to look for biosignatures. Investigations of ExoMars include measurement on molecular chirality. We show that chiral chloroalkanes, that have been identified on Mars, can be
Asma Merzougui, Agnes Banreti, Armelle L. Ernst, Guillaume Leseigneur, Uwe J. Meierhenrich   +4 more
wiley   +1 more source