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Gene targeting in Arabidopsis thaliana
Molecular and General Genetics MGG, 1992Gene targeting of a chromosomally integrated transgene in Arabidopsis thaliana is reported. A chimeric gene consisting of the promoter of the 35S RNA of CaMV, the polyadenylation signal of the octopine synthase gene and the coding region of the bacterial hygromycin phosphotransferase gene (hpt), which was rendered non-functional by deletion of 19 bp ...
Halfter, U., Morris, P., Willmitzer, L.
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Annual Review of Genetics, 1987
Arabidopsis thaliana is a small weed in the mustard family. It has been a convenient subject for studies in classical genetics for over forty years (35, 76). Recently, investigators have recognized that this flowering plant also has a genome size and genomic organization that recommend it for certain experiments in molecular genetics (41, 56, 57). As a
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Arabidopsis thaliana is a small weed in the mustard family. It has been a convenient subject for studies in classical genetics for over forty years (35, 76). Recently, investigators have recognized that this flowering plant also has a genome size and genomic organization that recommend it for certain experiments in molecular genetics (41, 56, 57). As a
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2021
Aims. Copper (Cu) is a micronutrient that has roles in photosynthesis, respiration, antioxidant activity, cell wall metabolism and hormone perception. Excess Cu is toxic and therefore its delivery has to be tightly regulated. Methods. Light and fluorescent microscopy, laser scanning confocal microscope. Results.
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Aims. Copper (Cu) is a micronutrient that has roles in photosynthesis, respiration, antioxidant activity, cell wall metabolism and hormone perception. Excess Cu is toxic and therefore its delivery has to be tightly regulated. Methods. Light and fluorescent microscopy, laser scanning confocal microscope. Results.
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Grafting with Arabidopsis thaliana
2016Generating chimeric organisms is an invaluable way to study cell-to-cell movement and non-cell-autonomous actions of molecules. Plant grafting is an ancient method of generating chimeric organisms and recently has been used to study the movement of hormones, proteins, and RNAs.
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Proteins are polyisoprenylated in Arabidopsis thaliana
Biochemical and Biophysical Research Communications, 2004Isoprenoid lipids were found to be covalently linked to proteins of Arabidopsis thaliana. Their identity (polyprenols: Prenol-9-11 with Pren-10 dominating and dolichols: Dol-15-17 with Dol-16 dominating) was confirmed by means of HPLC/ESI-MS with application of the multiple reaction monitoring technique as well as metabolic labeling of Arabidopsis ...
Malgorzata, Gutkowska +6 more
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In planta transformation of Arabidopsis thaliana
Molecular and General Genetics MGG, 1994Transformants of Arabidopsis thaliana can be generated without using tissue culture techniques by cutting primary and secondary inflorescence shoots at their bases and inoculating the wound sites with Agrobacterium tumefaciens suspensions. After three successive inoculations, treated plants are grown to maturity, harvested and the progeny screened for ...
Katavic, Vesna +4 more
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2015
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?????????? ???????????????????? ?????????????????? ??????????i?? ??? ????????i?? (Cd?????) ???? ??????????????i ??i?????????????? ?????????????????? ????i???????? ???? ??????????????i ?????????????????????? ??????????????. ?? ??????i?? ??????????i ????????????????, ???? NdSO??? i????i?????? ??i???? ???????????????? ??????????i??
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Chromosomal proteins of Arabidopsis thaliana
Plant Molecular Biology, 1988In plants with large genomes, each of the classes of the histones (H1, H2A, H2B, H3 and H4) are not unique polypeptides, but rather families of closely related proteins that are called histone variants. The small genome and preponderance of single-copy DNA in Arabidopsis thaliana has led us to ask if this plant has such families of histone variants. We
C P, Moehs, E F, McElwain, S, Spiker
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Accumulation of coumarins in Arabidopsis thaliana
Phytochemistry, 2006The biosynthesis of coumarins in plants is not well understood, although these metabolic pathways are often found in the plant kingdom. We report here the occurrence of coumarins in Arabidopsis thaliana ecotype Columbia. Considerably high levels of scopoletin and its beta-d-glucopyranoside, scopolin, were found in the wild-type roots.
Kosuke, Kai +4 more
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