Results 41 to 50 of about 28,641 (215)

Genome editing in archaeal viruses and endogenous viral protein purification [PDF]

STAR Protocols, 2021
Archaea-infecting viruses are morphologically and genomically among the most diverse entities. Unfortunately, they are also fairly understudied due to a lack of efficient genetic tools. Here, we present a detailed protocol for the CRISPR/Cas-based genome editing of the virus SIRV2 infecting the genus Sulfolobus, which could easily be adapted to other ...
Lauge Alfastsen, Xu Peng, Yuvaraj Bhoobalan-Chitty   +2 more
openaire   +4 more sources

Protein-Protein Interaction Changes in an Archaeal Light-Signal Transduction [PDF]

Journal of Biomedicine and Biotechnology, 2010
Negative phototaxis inNatronomonas pharaonisis initiated by transient interaction changes between photoreceptor and transducer.pharaonisphoborhodopsin (ppR; also calledpharaonissensory rhodopsin II,psR-II) and the cognate transducer protein,pHtrII, form a tight 2 : 2 complex in the unphotolyzed state, and the interaction is somehow altered during the ...
Kandori, Hideki, Sudo, Yuki, Furutani, Yuji   +2 more
openaire   +2 more sources

Identification of Archaea-specific chemotaxis proteins which interact with the flagellar apparatus

BMC Microbiology, 2009
Background Archaea share with bacteria the ability to bias their movement towards more favorable locations, a process known as taxis. Two molecular systems drive this process: the motility apparatus and the chemotaxis signal transduction system.
Müller Judith, Staudinger Wilfried F, Streif Stefan, Miller Arthur, Schlesner Matthias, Scheffer Beatrix, Siedler Frank, Oesterhelt Dieter   +7 more
doaj   +1 more source

Global analysis of viral infection in an archaeal model system

Frontiers in Microbiology, 2012
The origin and evolutionary relationship of viruses is poorly understood. This makes archaeal virus-host of particular interest because the hosts generally root near the base of phylogenetic trees, while some of the viruses have clear structural ...
Walid S. Maaty, Joseph eSteffens, Joshua eHeinemann, Alice C. Ortmann, Benjamin D. Reeves, Swapan K. Biswas, Edward A. Dratz, Paul A. Grieco, Mark J. Young, Brian eBothner   +9 more
doaj   +1 more source

Structure and function of archaeal histones. [PDF]

PLoS Genetics, 2018
The genomes of all organisms throughout the tree of life are compacted and organized in chromatin by association of chromatin proteins. Eukaryotic genomes encode histones, which are assembled on the genome into octamers, yielding nucleosomes.
Bram Henneman, Clara van Emmerik, Hugo van Ingen, Remus T Dame   +3 more
doaj   +1 more source

Structural conservation in a membrane-enveloped filamentous virus infecting a hyperthermophilic acidophile

Nature Communications, 2018
Only a few archaeal filamentous viruses have been structurally characterized. Here the authors describe the membrane-enveloped Sulfolobus filamentous virus 1 that infects Sulfolobus shibatae and present its 3.7 Å resolution cryo-EM structure, which ...
Ying Liu, Tomasz Osinski, Fengbin Wang, Mart Krupovic, Stefan Schouten, Peter Kasson, David Prangishvili, Edward H. Egelman   +7 more
doaj   +1 more source

Extracellular Vesicles of Streptococcus anginosus Mediate Gastritis via Epithelial Barrier Disruption and Macrophage‐driven Inflammation

Advanced Science, EarlyView.
Streptococcus anginosus extracellular vesicles (SA‐EVs) accumulate in gastric tissue, disrupt epithelial tight junctions, and induce gastritis characterized by neutrophil infiltration and elevated cytokines (TNF‐α, IL‐6, IL‐17A). Proteomics identifies TMPC and FBP62 as key SA‐EVs virulence factors; their genetic deletion attenuates inflammation ...
Ying Gong, Lina Duan, Jie Xiao, Yulu Deng, Hongxia Wang, Yajie Zhang, Xiumei Hu, Haifang Wang, Taixue An, Xin Li, Yurong Qiu, Lei Zheng, Haixia Li   +12 more
wiley   +1 more source

A Sirtuin‐1‐Targeted Gene‐Activating Tetrahedral DNA Attenuates Bladder Fibrosis by Restoring Mitophagy in Fibroblasts via the SIRT1‐FOXO3‐BNIP3 Axis

Advanced Science, EarlyView.
The SIRT1‐targeted saRNA‐delivering tetrahedral DNA (TSA) treatment effectively upregulates SIRT1 expression, which subsequently promotes FOXO3A deacetylation. This deacetylation event relieves FOXO3A's transcriptional repression on the BNIP3 gene, thereby initiating PINK1‐PARKIN‐dependent mitophagy.
Wei Wang, Ran Yan, Lede Lin, Lei Xiang, Xiaozhi Xia, Liao Peng, Xiaoshuai Gao, Jiawei Chen, Yang Xiong, Liang Zhou, Yunfeng Lin   +10 more
wiley   +1 more source

Disrupting Lipid Raft Microdomains to Block Polyploid Giant Cancer Cell Budding and Enhance Radiotherapy Response

Advanced Science, EarlyView.
Radiation induces polyploid giant cancer cells (PGCCs) that regenerate tumors through virus‐like budding. This process depends on a SNCG–FLOT2–CHMP4B signaling axis functioning in lipid raft microdomains. Disrupting these domains using statins or anti‐PCSK9 antibodies blocks PGCC budding, suppresses tumor repopulation, and enhances radiotherapy ...
Zheng Deng, Haoran Sun, Jin Cheng, Ruyi Zhao, Jianzhu Xie, Yanwei Song, Yucui Zhao, Chenwei Lin, Binjie Hu, Yanping Gong, Jun Lin, Sijia He, Yuntao Luo, Minghui Zhao, Yiwei Wang, Ming Jiao, Yuqin Yang, Jikun Li, Shujie Xia, Chuanyuan Li, Qian Huang   +20 more
wiley   +1 more source

Archaeal S-layer glycoproteins: Post-translational modification in the face of extremes

Frontiers in Microbiology, 2014
Corresponding to the sole or basic component of the surface (S)-layer surrounding the archaeal cell in most known cases, S-layer glycoproteins are in direct contact with the harsh environments that characterize niches where Archaea can thrive ...
Jerry eEichler, Lina eKandiba
doaj   +1 more source