Magnetically Agitated Nanoparticle-Based Batch Reactors for Biocatalysis with Immobilized Aspartate Ammonia-Lyase [PDF]
Catalysts, 2021 In this study, we investigated the influence of different modes of magnetic mixing on effective enzyme activity of aspartate ammonia-lyase from Pseudomonas fluorescens immobilized onto epoxy-functionalized magnetic nanoparticles by covalent binding (AAL-MNP).
Ali Obaid Imarah +7 more
openaire +3 more sources
Biocatalytic Enantioselective Synthesis of N‐Substituted Aspartic Acids by Aspartate Ammonia Lyase [PDF]
Chemistry – A European Journal, 2008 AbstractThe gene encoding aspartate ammonia lyase (aspB) from Bacillus sp. YM55‐1 has been cloned and overexpressed, and the recombinant enzyme containing a C‐terminal His6 tag has been purified to homogeneity and subjected to kinetic characterization. Kinetic studies have shown that the His6 tag does not affect AspB activity.
Weiner, Barbara +3 more
openaire +3 more sources
The VarA-CsrA regulatory pathway influences cell shape in Vibrio cholerae.
PLoS Genetics, 2022 Despite extensive studies on the curve-shaped bacterium Vibrio cholerae, the causative agent of the diarrheal disease cholera, its virulence-associated regulatory two-component signal transduction system VarS/VarA is not well understood.
Leonardo F Lemos Rocha +6 more
doaj +1 more source
Biochemical, kinetic, and spectroscopic characterization of Ruegeria pomeroyi DddW - A mononuclear iron-dependent DMSP lyase [PDF]
, 2015 The osmolyte dimethylsulfoniopropionate (DMSP) is a key nutrient in marine environments and its catabolism by bacteria through enzymes known as DMSP lyases generates dimethylsulfide (DMS), a gas of importance in climate regulation, the sulfur cycle, and ...
Brummett, Adam E +4 more
core +8 more sources
CodY, a pleiotropic regulator, influences multicellular behaviour and efficient production of virulence factors in Bacillus cereus [PDF]
, 2012 In response to nutrient limitation in the environment, the global transcriptional regulator CodY modulates various pathways in low G+C Gram-positive bacteria.
Basset, Coraline +6 more
core +12 more sources
Genomic and experimental evidence for multiple metabolic functions in the RidA/YjgF/YER057c/UK114 (Rid) protein family. [PDF]
, 2015 BackgroundIt is now recognized that enzymatic or chemical side-reactions can convert normal metabolites to useless or toxic ones and that a suite of enzymes exists to mitigate such metabolite damage.
Cooper, Arthur JL +8 more
core +4 more sources
Metabolites, 2023 Different southern grape (Muscadine) genotypes (Muscadinia rotundifolia Michx.) were evaluated for their contents of metabolites in ripe berries. The metabolome study identified 331 metabolites in ripening skin and seed tissues. The major chemical groups
Ahmed G. Darwish +3 more
doaj +1 more source
BMC Gastroenterology, 2022 Background Ornithine transcarbamylase deficiency (OTCD) is most common among urea cycle disorders (UCDs), defined by defects in enzymes associated with ureagenesis.
Koji Imoto +9 more
doaj +1 more source
Current knowledge and recent advances in understanding metabolism of the model cyanobacterium Synechocystis sp. PCC 6803 [PDF]
, 2020 Cyanobacteria are key organisms in the global ecosystem, useful models for studying metabolic and physiological processes conserved in photosynthetic organisms, and potential renewable platforms for production of chemicals.
Lea-Smith, David +2 more
core +3 more sources
The genome of Pelobacter carbinolicus reveals surprising metabolic capabilities and physiological features [PDF]
, 2012 Background The bacterium Pelobacter carbinolicus is able to grow by fermentation, syntrophic hydrogen/formate transfer, or electron transfer to sulfur from short-chain alcohols, hydrogen or formate; it does not oxidize acetate and is not known to ferment
Cliff S Han +7 more
core +2 more sources