Decoding the substrate specificity landscape of a promiscuous enzyme through multi-substrate mutational scanning [PDF]
Nature CommunicationsSubstrate specificity is a defining feature of enzyme function, but its molecular underpinnings remain difficult to decode and engineer. Here, we leverage enzyme proximity sequencing (EP-Seq) to systematically map how single-point and combinatorial ...
Rosario Vanella +3 more
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Structural basis of SIRT7 nucleosome engagement and substrate specificity [PDF]
Nature CommunicationsChromatin-modifying enzymes target distinct residues within histones to finetune gene expression profiles. SIRT7 is an NAD+-dependent deacylase often deregulated in cancer, which deacetylates either H3 lysine 36 (H3K36) or H3K18 with high specificity ...
Carlos Moreno-Yruela +6 more
doaj +3 more sources
Discovery and substrate specificity engineering of nucleotide halogenases [PDF]
Nature CommunicationsC2′-halogenation has been recognized as an essential modification to enhance the drug-like properties of nucleotide analogs. The direct C2ʹ-halogenation of the nucleotide 2′-deoxyadenosine-5′-monophosphate (dAMP) has recently been achieved using the Fe ...
Jie Ni +4 more
doaj +2 more sources
Structural Analysis and Substrate Specificity of D-Carbamoylase from Pseudomonas [PDF]
BioTechThe synthesis of enantiomeric forms of D-amino acids can be achieved by a two-step “hydantoinase process” based on the sequential catalysis of substrates by specific enzymes, D-carbamoylase and D-hydantoinase. Here, we describe the structural features of
Marina Paronyan +9 more
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Substrate Specificity of SARS-CoV-2 Nsp10-Nsp16 Methyltransferase
Viruses, 2021 The ongoing COVID-19 pandemic exemplifies the general need to better understand viral infections. The positive single-strand RNA genome of its causative agent, the SARS coronavirus 2 (SARS-CoV-2), encodes all viral enzymes.
Roberto Benoni +5 more
doaj +1 more source
Profiling of substrate specificity of SARS-CoV 3CL. [PDF]
PLoS ONE, 2010 BACKGROUND: The 3C-like protease (3CL(pro)) of severe acute respiratory syndrome-coronavirus is required for autoprocessing of the polyprotein, and is a potential target for treating coronaviral infection.
Chi-Pang Chuck +5 more
doaj +1 more source
Tocopherol Cyclases-Substrate Specificity and Phylogenetic Relations. [PDF]
PLoS ONE, 2016 In the present studies, we focused on substrate specificity of tocopherol cyclase, the key enzyme in the biosynthesis of the tocopherols and plastochromanol-8, the main plant lipid antioxidants, with special emphasis on the preference for tocopherols and
Jolanta Dłużewska +5 more
doaj +1 more source
In silico prediction of mutant HIV-1 proteases cleaving a target sequence [PDF]
, 2014 HIV-1 protease represents an appealing system for directed enzyme re-design, since it has various different endogenous targets, a relatively simple structure and it is well studied.
De Vico, Luca +3 more
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Probing ADAMTS13 substrate specificity using phage display. [PDF]
PLoS ONE, 2015 Von Willebrand factor (VWF) is a large, multimeric protein that regulates hemostasis by tethering platelets to the subendothelial matrix at sites of vascular damage.
Karl C Desch +7 more
doaj +1 more source
Substrate specificity of prostate-specific membrane antigen [PDF]
Bioorganic & Medicinal Chemistry, 2007 A series of putative dipeptide substrates of prostate-specific membrane antigen (PSMA) was prepared that explored alpha- and beta/gamma-linked acidic residues at the P1 position and various chromophores at the P2 position, while keeping the P1' residue constant as L-Glu.
Anderson, Marc O +6 more
openaire +3 more sources