Results 1 to 10 of about 467,506 (265)

Investigating substrate specificity in arogenate versus prephenate dehydratases [PDF]

BMC Biology
Background Phenylalanine is an essential aromatic amino acid that can only be synthesized de novo by microorganisms and plants. In microorganisms, phenylalanine is synthesized through the prephenate pathway, requiring the activity of a prephenate ...
Emily J. Clayton, Megan Smith-Uffen, Travis W. Tribble, Martin L. Duennwald, Susanne E. Kohalmi   +4 more
doaj   +2 more sources

Decoding the substrate specificity landscape of a promiscuous enzyme through multi-substrate mutational scanning [PDF]

Nature Communications
Substrate specificity is a defining feature of enzyme function, but its molecular underpinnings remain difficult to decode and engineer. Here, we leverage enzyme proximity sequencing (EP-Seq) to systematically map how single-point and combinatorial ...
Rosario Vanella, Sean Boult, Christoph Küng, Michael A. Nash   +3 more
doaj   +2 more sources

Structural basis of SIRT7 nucleosome engagement and substrate specificity [PDF]

Nature Communications
Chromatin-modifying enzymes target distinct residues within histones to finetune gene expression profiles. SIRT7 is an NAD+-dependent deacylase often deregulated in cancer, which deacetylates either H3 lysine 36 (H3K36) or H3K18 with high specificity ...
Carlos Moreno-Yruela, Babatunde E. Ekundayo, Polina N. Foteva, Dongchun Ni, Esther Calvino-Sanles, Henning Stahlberg, Beat Fierz   +6 more
doaj   +3 more sources

Substrate Specificity of SARS-CoV-2 Nsp10-Nsp16 Methyltransferase

Viruses, 2021
The ongoing COVID-19 pandemic exemplifies the general need to better understand viral infections. The positive single-strand RNA genome of its causative agent, the SARS coronavirus 2 (SARS-CoV-2), encodes all viral enzymes.
Roberto Benoni, Petra Krafcikova, Marek R. Baranowski, Joanna Kowalska, Evzen Boura, Hana Cahová   +5 more
doaj   +1 more source

Profiling of substrate specificity of SARS-CoV 3CL. [PDF]

PLoS ONE, 2010
BACKGROUND: The 3C-like protease (3CL(pro)) of severe acute respiratory syndrome-coronavirus is required for autoprocessing of the polyprotein, and is a potential target for treating coronaviral infection.
Chi-Pang Chuck, Lin-Tat Chong, Chao Chen, Hak-Fun Chow, David Chi-Cheong Wan, Kam-Bo Wong   +5 more
doaj   +1 more source

The Substrate Specificity of Pantethinase [PDF]

European Journal of Biochemistry, 1973
The substrate specificty of pantethinase, the mammalian enzyme which has been isolated from horse kidney cortex, has been studied. The real substrate has been found to be pantetheine in the reduced form, and the involvement of one or more—SH groups of the enzyme is also considered.A number of other compounds, chemically related to pantetheine, has also
S, Duprè, M A, Rosei, L, Bellussi, E, Del Grosso, D, Cavallini   +4 more
openaire   +2 more sources

Tocopherol Cyclases-Substrate Specificity and Phylogenetic Relations. [PDF]

PLoS ONE, 2016
In the present studies, we focused on substrate specificity of tocopherol cyclase, the key enzyme in the biosynthesis of the tocopherols and plastochromanol-8, the main plant lipid antioxidants, with special emphasis on the preference for tocopherols and
Jolanta Dłużewska, Renata Szymańska, Michal Gabruk, Peter B Kós, Beatrycze Nowicka, Jerzy Kruk   +5 more
doaj   +1 more source

Probing ADAMTS13 substrate specificity using phage display. [PDF]

PLoS ONE, 2015
Von Willebrand factor (VWF) is a large, multimeric protein that regulates hemostasis by tethering platelets to the subendothelial matrix at sites of vascular damage.
Karl C Desch, Colin Kretz, Andrew Yee, Robert Gildersleeve, Kristin Metzger, Nidhi Agrawal, Jane Cheng, David Ginsburg   +7 more
doaj   +1 more source

Substrate Specificity of the SecB Chaperone [PDF]

Journal of Biological Chemistry, 1999
The bacterial chaperone SecB assists translocation of proteins across the inner membrane. The mechanism by which it differentiates between secretory and cytosolic proteins is poorly understood. To identify its binding motif, we screened 2688 peptides covering sequences of 23 proteins for SecB binding.
Knoblauch, Nicola T. M., Rüdiger, Stefan, Schönfeld, Hans-Joachim, Driessen, Arnold J. M., Schneider-Mergener, Jens, Bukau, Bernd   +5 more
openaire   +4 more sources

Substrate specificity of rat brain ceramidase

Journal of Lipid Research, 2002
In this study, the substrate specificity of a newly identified rat brain ceramidase (CDase) was investigated. To this end, the major functional groups and stereochemistry of ceramide (Cer) were evaluated for their influence on the hydrolysis of substrate
Samer El Bawab, Julnar Usta, Patrick Roddy, Zdzislaw M. Szulc, Alicja Bielawska, Yusuf A. Hannun   +5 more
doaj   +1 more source