Results 1 to 10 of about 494,088 (314)
Substrate specificity of haloalkane dehalogenases [PDF]
Biochemical Journal, 2011 An enzyme's substrate specificity is one of its most important characteristics. The quantitative comparison of broad-specificity enzymes requires the selection of a homogenous set of substrates for experimental testing, determination of substrate-specificity data and analysis using multivariate statistics.
Tana, Koudelakova +6 more
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Substrate Specificity of SARS-CoV-2 Nsp10-Nsp16 Methyltransferase
Viruses, 2021 The ongoing COVID-19 pandemic exemplifies the general need to better understand viral infections. The positive single-strand RNA genome of its causative agent, the SARS coronavirus 2 (SARS-CoV-2), encodes all viral enzymes.
Roberto Benoni +5 more
doaj +1 more source
Profiling of substrate specificity of SARS-CoV 3CL. [PDF]
PLoS ONE, 2010 BACKGROUND: The 3C-like protease (3CL(pro)) of severe acute respiratory syndrome-coronavirus is required for autoprocessing of the polyprotein, and is a potential target for treating coronaviral infection.
Chi-Pang Chuck +5 more
doaj +1 more source
The Substrate Specificity of Pantethinase [PDF]
European Journal of Biochemistry, 1973 The substrate specificty of pantethinase, the mammalian enzyme which has been isolated from horse kidney cortex, has been studied. The real substrate has been found to be pantetheine in the reduced form, and the involvement of one or more—SH groups of the enzyme is also considered.A number of other compounds, chemically related to pantetheine, has also
S, Duprè +4 more
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Tocopherol Cyclases-Substrate Specificity and Phylogenetic Relations. [PDF]
PLoS ONE, 2016 In the present studies, we focused on substrate specificity of tocopherol cyclase, the key enzyme in the biosynthesis of the tocopherols and plastochromanol-8, the main plant lipid antioxidants, with special emphasis on the preference for tocopherols and
Jolanta Dłużewska +5 more
doaj +1 more source
Probing ADAMTS13 substrate specificity using phage display. [PDF]
PLoS ONE, 2015 Von Willebrand factor (VWF) is a large, multimeric protein that regulates hemostasis by tethering platelets to the subendothelial matrix at sites of vascular damage.
Karl C Desch +7 more
doaj +1 more source
Substrate Specificity of the SecB Chaperone [PDF]
Journal of Biological Chemistry, 1999 The bacterial chaperone SecB assists translocation of proteins across the inner membrane. The mechanism by which it differentiates between secretory and cytosolic proteins is poorly understood. To identify its binding motif, we screened 2688 peptides covering sequences of 23 proteins for SecB binding.
Knoblauch, Nicola T. M. +5 more
openaire +4 more sources
Substrate specificity of rat brain ceramidase
Journal of Lipid Research, 2002 In this study, the substrate specificity of a newly identified rat brain ceramidase (CDase) was investigated. To this end, the major functional groups and stereochemistry of ceramide (Cer) were evaluated for their influence on the hydrolysis of substrate
Samer El Bawab +5 more
doaj +1 more source
Evolution of substrate specificity in a recipient's enzyme following horizontal gene transfer [PDF]
, 2013 Despite the prominent role of horizontal gene transfer (HGT) in shaping bacterial metabolism, little is known about the impact of HGT on the evolution of enzyme function.
Noda-Garcia, Lianet +6 more
core +1 more source
Structure-based engineering of substrate specificity for pinoresinol-lariciresinol reductases
Nature Communications, 2021 Pinoresinol–lariciresinol reductases (PLRs) are enzymes involved in the lignan biosynthesis. Here, crystal structures of three PLRs in the apo, substrate-bound and product-bound states, and accompanying mutagenesis provide insight into PLRs catalytic ...
Ying Xiao +13 more
doaj +1 more source