Investigating substrate specificity in arogenate versus prephenate dehydratases [PDF]
BMC BiologyBackground Phenylalanine is an essential aromatic amino acid that can only be synthesized de novo by microorganisms and plants. In microorganisms, phenylalanine is synthesized through the prephenate pathway, requiring the activity of a prephenate ...
Emily J. Clayton +4 more
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Graph neural networks can predict ketosynthase substrate specificity [PDF]
Scientific ReportsModular polyketide synthases are a class of biosynthetic machinery responsible for the production of a chemically diverse range of natural products, including essential medicines.
Maxim Walmsley +3 more
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Decoding the substrate specificity landscape of a promiscuous enzyme through multi-substrate mutational scanning [PDF]
Nature CommunicationsSubstrate specificity is a defining feature of enzyme function, but its molecular underpinnings remain difficult to decode and engineer. Here, we leverage enzyme proximity sequencing (EP-Seq) to systematically map how single-point and combinatorial ...
Rosario Vanella +3 more
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Substrate specificity of dehydrogenases.
, 2022 Substrate specificity of dehydrogenases.
Qipeng Yuan (133178) +8 more
core +1 more source
Evolution of substrate specificity in a recipient's enzyme following horizontal gene transfer [PDF]
, 2013 Despite the prominent role of horizontal gene transfer (HGT) in shaping bacterial metabolism, little is known about the impact of HGT on the evolution of enzyme function.
Noda-Garcia, Lianet +6 more
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Computational Protein Design Force Field Optimization: A Negative Design Approach [PDF]
, 2007 An accurate force field is essential to computational protein design and protein folding studies. Proper force field tuning is problematic, however, due in part to the incomplete modeling of the unfolded state.
Alvizo, Oscar
core +1 more source
Substrate specificity of the AKR.
, 2021 Substrate specificity of the AKR.
Xiaoli Fu (316464) +2 more
core +1 more source
Do Amino Acid Antiporters Have Asymmetric Substrate Specificity?
Biomolecules, 2023 Amino acid antiporters mediate the 1:1 exchange of groups of amino acids. Whether substrate specificity can be different for the inward and outward facing conformation has not been investigated systematically, although examples of asymmetric transport ...
Gregory Gauthier-Coles +3 more
doaj +1 more source
Substrate specificity of human ceramide kinase
Journal of Lipid Research, 2005 Previous studies in our laboratory have established ceramide kinase (CERK) as a critical mediator of eicosanoid synthesis. To date, CERK has not been well characterized in vitro.
Dayanjan S. Wijesinghe +5 more
doaj +1 more source
Dissecting the Cytochrome P450 OleP Substrate Specificity: Evidence for a Preferential Substrate
Biomolecules, 2020 The cytochrome P450 OleP catalyzes the epoxidation of aliphatic carbons on both the aglycone 8.8a-deoxyoleandolide (DEO) and the monoglycosylated L-olivosyl-8.8a-deoxyoleandolide (L-O-DEO) intermediates of oleandomycin biosynthesis.
Giacomo Parisi +10 more
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