Results 131 to 140 of about 3,875,152 (312)

Rapid and scalable detection of synthetic mRNA byproducts using polynucleotide phosphorylase and polythymidine oligonucleotides

RNA Biology
Production and storage of synthetic mRNA can introduce a variety of byproducts which reduce the overall integrity and functionality of mRNA vaccines and therapeutics.
Francis Combes, Thanh-Huong Bui, Frida J. Pettersson, Sjoerd Hak   +3 more
doaj   +1 more source

Translophagy—A potential link between autophagy impairment and translational errors

FEBS Letters, EarlyView.
Neurodegenerative diseases are characterised by the accumulation of abnormal proteins and protein aggregates, but their origin often remains unknown. We propose that selective autophagy removes damaged protein‐making machinery, preventing errors during protein synthesis.
Mykola V. Korolchuk, Gailing Ma, Robin Antrobus, Diego Manni, Marco Trevisan‐Herraz, Mustafa Khwaja, Max Brown, Gavin D. Richardson, Masaaki Komatsu, Daniel Erskine, Viktor I. Korolchuk, Tetsushi Kataura   +11 more
wiley   +1 more source

Measuring deiodinase activity: a need for standardization?

European Thyroid Journal
Thyroid hormones are produced in the thyroid gland and metabolized in the peripheral tissues. The major pathway of thyroid hormone metabolism is the removal of an iodine atom from the phenolic or tyrosyl ring by deiodinating enzymes, the so-called ...
Joëlle Wiersema, Petra Mohácsik, Balázs Gereben, Arturo Hernandez, Selmar Leeuwenburgh, Marcel Meima, W Edward Visser, Samuel Refetoff, Antonio Bianco, Federico Salas-Lucia, Anita Boelen   +10 more
doaj   +1 more source

Structural and biochemical analysis of a B12 superbinder

FEBS Letters, EarlyView.
BtuG proteins are vitamin B12 scavengers in Bacteroides thetaiotaomicron, a dominant human gut bacterium. We present crystal structures of three BtuG homologs bound to cobalamin and its precursor cobinamide, revealing picomolar binding affinities, among the highest known for any natural protein.
Jose M. Martinez Felices, Jacob J. Whittaker, Dirk J. Slotboom, Albert Guskov   +3 more
wiley   +1 more source

Fluorometric and Colorimetric Biosensors for the Assay of Cholinesterase Inhibitors

Sensors
Cholinesterases, specifically acetylcholinesterase (AChE) and butyrylcholinesterase (BChE), play critical roles in neurotransmission and are key targets for inhibitors with therapeutic and toxicological significance.
Miroslav Pohanka
doaj   +1 more source

Real-time PCR quantitation of hepatitis B virus DNA using automated sample preparation and murine cytomegalovirus internal control [PDF]

, 2005
Quantitation of circulating hepatitis B virus (HBV) DNA is important for monitoring disease progression and for assessing the response to antiviral therapy. Several commercial and 'in house' assays for HBV DNA quantitation have been described but many of
Tedder, RS, Ayliffe, U, Grant, PR, Ferns, RB, Garson, JA   +4 more
core  

Structures of mycobacterial 3‐methylcrotonyl‐CoA carboxylase reveal carrier‐domain translocation between catalytic sites

FEBS Letters, EarlyView.
Mycobacterial 3‐methylcrotonyl‐CoA carboxylase uses a mobile biotin‐carrying domain to shuttle a carboxyl group between two catalytic sites, enabling carboxylation of 3‐methylcrotonyl‐CoA during leucine breakdown. Cryo‐electron microscopy captures the carrier at both sites and reveals an inward loop movement that may prevent futile rebinding to the ...
Ajit Yadav, Bogdan I. Florea, Sebastian Geibel   +2 more
wiley   +1 more source

Correlation of Urine Ammonia Excretion With Renal Function in Healthy Cats and Cats With Kidney Disease

Journal of Veterinary Internal Medicine
Background Inadequate ammonia excretion is thought to drive the development of metabolic acidosis in people with CKD and to correlate with worse outcomes.
Eleanor E. Brown, Kirsten L. Cooke, Rebeca A. Castro, Alexis Cooper, Andrew J. Specht, Autumn N. Harris   +5 more
doaj   +1 more source

From junk to function — How weak selection in eukaryotes builds new parts and drives genomic complexity

FEBS Letters, EarlyView.
How do genomes gain new functional parts? In eukaryotes, which tend to evolve under weak selection, much of the genome is junk. Palazzo and Qiu borrow the logic of Markov chains to show how non‐functional DNA becomes functional through the appearance of intermediate states, which arise due to epistasis, buffering, and biochemical messiness, allowing ...
Alexander F. Palazzo, Yi Qiu
wiley   +1 more source

Electrochemical Detection of Thrombin Activity Using an Electrogenic Substrate: Toward Rapid Coagulation Monitoring

ChemElectroChem
Monitoring blood coagulation is critical for assessing bleeding and cardiac disorders. A variety of bioanalytical techniques for coagulation analysis are based on measuring the activity of a key enzyme, thrombin.
Sina Ardalan, Anna Ignaszak
doaj   +1 more source