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Cell Sensitivity Assays: The MTT Assay
2011The MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assay is based on the conversion of MTT into formazan crystals by living cells, which determines mitochondrial activity. Since for most cell populations the total mitochondrial activity is related to the number of viable cells, this assay is broadly used to measure the in vitro ...
van Meerloo, J. +2 more
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Cold Spring Harbor Protocols, 2010
INTRODUCTIONWhen a transient or stable transfection assay is developed for a promoter, a primary objective is to quantify promoter strength. Because transfection efficiency in such assays can be low, promoters are commonly fused to heterologous reporter genes that encode enzymes that can be quantified using highly sensitive assays. The reporter protein’
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INTRODUCTIONWhen a transient or stable transfection assay is developed for a promoter, a primary objective is to quantify promoter strength. Because transfection efficiency in such assays can be low, promoters are commonly fused to heterologous reporter genes that encode enzymes that can be quantified using highly sensitive assays. The reporter protein’
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2009
Genomic representations using ligation-mediated PCR have been used successfully as the foundation for a number of high-throughput assays. HpaII tiny fragment enrichment by ligation-mediated PCR (HELP) is an example of the use of such representations to study cytosine methylation in the genome.
Mayumi, Oda, John M, Greally
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Genomic representations using ligation-mediated PCR have been used successfully as the foundation for a number of high-throughput assays. HpaII tiny fragment enrichment by ligation-mediated PCR (HELP) is an example of the use of such representations to study cytosine methylation in the genome.
Mayumi, Oda, John M, Greally
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Current Opinion in Biotechnology, 1991
The past year or so has seen the development of new enzyme assays, as well as the improvement of existing ones. Assays are becoming more rapid and sensitive as a result of modifications such as amplification of the enzyme product(s). Recombinant DNA technology is now being recognized as a particularly useful tool in the search for improved assay ...
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The past year or so has seen the development of new enzyme assays, as well as the improvement of existing ones. Assays are becoming more rapid and sensitive as a result of modifications such as amplification of the enzyme product(s). Recombinant DNA technology is now being recognized as a particularly useful tool in the search for improved assay ...
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1978
Many investigators have attempted to determine whether a relationship exists between excessive activity of the hemostatic mechanism and the atherosclerotic process. A major difficulty encountered in unraveling this puzzle has been the lack of reliable techniques for quantifying pertinent changes in blood coagulability.
R D, Rosenberg +3 more
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Many investigators have attempted to determine whether a relationship exists between excessive activity of the hemostatic mechanism and the atherosclerotic process. A major difficulty encountered in unraveling this puzzle has been the lack of reliable techniques for quantifying pertinent changes in blood coagulability.
R D, Rosenberg +3 more
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2006
A large number of methods devoted to the identification of apoptotic cells and the analysis of the morphological, biochemical, and molecular changes that take place during this universal biological process have been developed. Apoptotic cells are recognized on the basis of their reduced DNA content and morphological changes that include nuclear ...
Marcela, Oancea +3 more
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A large number of methods devoted to the identification of apoptotic cells and the analysis of the morphological, biochemical, and molecular changes that take place during this universal biological process have been developed. Apoptotic cells are recognized on the basis of their reduced DNA content and morphological changes that include nuclear ...
Marcela, Oancea +3 more
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The investigation of yellow fever suspected cases is important for both epidemiological surveillance and for the timely adoption of supportive therapy. The immunofluorescence assay (IFA), a technique based on the detection of specific targets by fluorescent dye-tagged antibodies, may be useful for the serological diagnosis of yellow fever and/or for ...
Andréa Nazaré Monteiro Rangel, da Silva +1 more
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Andréa Nazaré Monteiro Rangel, da Silva +1 more
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The MTT Assay: Utility, Limitations, Pitfalls, and Interpretation in Bulk and Single-Cell Analysis
International Journal of Molecular Sciences, 2021Ivan Kempson +2 more
exaly
2001
Angiogenesis-the formation of a vascular network-is essential for the support of a developing tumor when simple diffusion of nutrients is impossible. The ability of a solid tumor to achieve metabolic needs beyond simple diffusion is dependent on the development of this neovascular network.
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Angiogenesis-the formation of a vascular network-is essential for the support of a developing tumor when simple diffusion of nutrients is impossible. The ability of a solid tumor to achieve metabolic needs beyond simple diffusion is dependent on the development of this neovascular network.
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2003
This chapter describes a quantitative assay for the streptococcal superantigen, streptococcal pyrogenic exotoxin A (SPEA), which can be used in broth, tissue-culture media, and certain sera. The protocol can be adapted to allow measurement of any bacterial superantigen or protein toxin, using different oligonucleotides to amplify the coding sequences ...
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This chapter describes a quantitative assay for the streptococcal superantigen, streptococcal pyrogenic exotoxin A (SPEA), which can be used in broth, tissue-culture media, and certain sera. The protocol can be adapted to allow measurement of any bacterial superantigen or protein toxin, using different oligonucleotides to amplify the coding sequences ...
openaire +2 more sources