Results 251 to 260 of about 3,875,152 (312)
Some of the next articles are maybe not open access.
Chem. Commun., 2008
Enzyme assays are analytical tools to visualize enzyme activities. In recent years a large variety of enzyme assays have been developed to assist the discovery and optimization of industrial enzymes, in particular for "white biotechnology" where selective enzymes are used with great success for economically viable, mild and environmentally benign ...
Reymond, Jean-Louis +2 more
openaire +4 more sources
Enzyme assays are analytical tools to visualize enzyme activities. In recent years a large variety of enzyme assays have been developed to assist the discovery and optimization of industrial enzymes, in particular for "white biotechnology" where selective enzymes are used with great success for economically viable, mild and environmentally benign ...
Reymond, Jean-Louis +2 more
openaire +4 more sources
Expert Opinion on Drug Discovery, 2010
By selecting the most appropriate bioluminescence assay, the researcher can study the underlying molecular mechanisms of a physiological system and the effects of a drug throughout the body.This review covers three luciferase assay systems: the multicolor luciferase assay, secreted luciferase assay and imaging luciferase assay. These assays are applied
Yoshihiro, Nakajima, Yoshihiro, Ohmiya
openaire +2 more sources
By selecting the most appropriate bioluminescence assay, the researcher can study the underlying molecular mechanisms of a physiological system and the effects of a drug throughout the body.This review covers three luciferase assay systems: the multicolor luciferase assay, secreted luciferase assay and imaging luciferase assay. These assays are applied
Yoshihiro, Nakajima, Yoshihiro, Ohmiya
openaire +2 more sources
Analytical Biochemistry, 1979
Abstract Two methods are described for the assay of sulfotransferases which are active with sulfate acceptors bearing the hydroxyl functional group. Assays were developed for enzymes which transfer sulfate from 3′-phosphoadenosine–5′-phosphosulfate (PAPS) to sterols, phenols, and simple alcohols thereby forming the corresponding sulfate esters.
R D, Sekura +3 more
openaire +2 more sources
Abstract Two methods are described for the assay of sulfotransferases which are active with sulfate acceptors bearing the hydroxyl functional group. Assays were developed for enzymes which transfer sulfate from 3′-phosphoadenosine–5′-phosphosulfate (PAPS) to sterols, phenols, and simple alcohols thereby forming the corresponding sulfate esters.
R D, Sekura +3 more
openaire +2 more sources
Cell Sensitivity Assays: Clonogenic Assay
2003The use of cell culture systems to assess the toxicity of anticancer agents began over 50 years ago following the observation of the antineoplastic effects of nitrogen mustard (1). There are a wide variety of assays designed to evaluate cellular drug sensitivity described in the literature.
openaire +3 more sources
Cell Sensitivity Assays: The MTT Assay
2003The clonogenic assay described in Chapter 2 is not suitable for all cell lines. Many adherent cell lines do not form colonies and clearly it is not applicable to non-adherent cell lines. Furthermore, it is slow and time consuming. This chapter describes an alternative cytotoxicity assay that has a number of advantages when compared with a clonogenic ...
openaire +3 more sources
Cold Spring Harbor Protocols, 2010
INTRODUCTION When a transient or stable transfection assay is developed for a promoter, a primary objective is to quantify promoter strength. Because transfection efficiency in such assays can be low, promoters are commonly fused to heterologous reporter genes that encode enzymes that can be quantified using ...
openaire +2 more sources
INTRODUCTION When a transient or stable transfection assay is developed for a promoter, a primary objective is to quantify promoter strength. Because transfection efficiency in such assays can be low, promoters are commonly fused to heterologous reporter genes that encode enzymes that can be quantified using ...
openaire +2 more sources
2009
Genomic representations using ligation-mediated PCR have been used successfully as the foundation for a number of high-throughput assays. HpaII tiny fragment enrichment by ligation-mediated PCR (HELP) is an example of the use of such representations to study cytosine methylation in the genome.
Mayumi, Oda, John M, Greally
openaire +2 more sources
Genomic representations using ligation-mediated PCR have been used successfully as the foundation for a number of high-throughput assays. HpaII tiny fragment enrichment by ligation-mediated PCR (HELP) is an example of the use of such representations to study cytosine methylation in the genome.
Mayumi, Oda, John M, Greally
openaire +2 more sources
Current Opinion in Biotechnology, 1991
The past year or so has seen the development of new enzyme assays, as well as the improvement of existing ones. Assays are becoming more rapid and sensitive as a result of modifications such as amplification of the enzyme product(s). Recombinant DNA technology is now being recognized as a particularly useful tool in the search for improved assay ...
openaire +2 more sources
The past year or so has seen the development of new enzyme assays, as well as the improvement of existing ones. Assays are becoming more rapid and sensitive as a result of modifications such as amplification of the enzyme product(s). Recombinant DNA technology is now being recognized as a particularly useful tool in the search for improved assay ...
openaire +2 more sources
1978
Many investigators have attempted to determine whether a relationship exists between excessive activity of the hemostatic mechanism and the atherosclerotic process. A major difficulty encountered in unraveling this puzzle has been the lack of reliable techniques for quantifying pertinent changes in blood coagulability.
R D, Rosenberg +3 more
openaire +2 more sources
Many investigators have attempted to determine whether a relationship exists between excessive activity of the hemostatic mechanism and the atherosclerotic process. A major difficulty encountered in unraveling this puzzle has been the lack of reliable techniques for quantifying pertinent changes in blood coagulability.
R D, Rosenberg +3 more
openaire +2 more sources
2006
A large number of methods devoted to the identification of apoptotic cells and the analysis of the morphological, biochemical, and molecular changes that take place during this universal biological process have been developed. Apoptotic cells are recognized on the basis of their reduced DNA content and morphological changes that include nuclear ...
Marcela, Oancea +3 more
openaire +2 more sources
A large number of methods devoted to the identification of apoptotic cells and the analysis of the morphological, biochemical, and molecular changes that take place during this universal biological process have been developed. Apoptotic cells are recognized on the basis of their reduced DNA content and morphological changes that include nuclear ...
Marcela, Oancea +3 more
openaire +2 more sources