Reconstructing enzyme evolution by protein engineering
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler +2 more
wiley +1 more source
Dynamic in situ analysis of modern and ancient flax yarns: Impact of microstructure and aging
Flax yarns are widely used in composites and textiles, yet their mechanical performance is limited by factors like microstructure and durability, necessitating in-depth investigation of their tensile behavior.
Vasuki Rajakumaran +5 more
doaj +1 more source
Conserved binding mode but diverse interfaces of MreC‐PBP2 interactions
The crystal structure of abMreC reveals a conserved two β‐barrel architecture and provides structural insights into its role within the bacterial elongasome. The abMreC–abPBP2 complex model identifies the molecular basis of MreC‐mediated PBP2 recognition, contributing to the regulation of peptidoglycan synthesis.
Hyunseok Jang +4 more
wiley +1 more source
Notes on some statistical aspects of pottery quantification [PDF]
The quantification of finds assemblages from excavations, as an aid to the comparative study of assemblages and sites, is a developing and increasingly important aspect of post-excavation analysis.
Cool, HEM, Baxter, MJ
core
Reef fishes of Saba Bank, Netherlands Antilles : Assemblage structure across a gradient of habitat types [PDF]
Saba Bank is a 2,200 km2 submerged carbonate platform in the northeastern Caribbean Sea off Saba Island, Netherlands Antilles. The presence of reef-like geomorphic features and significant shelf edge coral development on Saba Bank have led to the ...
Paul C Hoetjes +12 more
core +3 more sources
Seasonal dynamics of soft-bottom polychaetes in Izmir Bay (Aegean Sea, Eastern Mediterranean)
Seasonal dynamics of soft-bottom polychaetes were investigated at 29 stations (depths: 8-77 m) in Izmir Bay (Aegean Sea, eastern Mediterranean) between 1997 and 2002.
Zeki Ergen +3 more
doaj +1 more source
Synergistic perspectives—How single‐molecule biophysics complement biochemical understanding
In this review, we discuss how ensemble biochemistry and single‐molecule approaches are complementary, outline commonly used single‐molecule techniques, and illustrate their relevance through two representative case studies: chromatin organization by SMC complexes and pathway choice during DNA double‐strand break repair.
Sara De Bragança +2 more
wiley +1 more source
Encapsulins are protein nanocompartments that play an important role in iron storage. In the Myxococcus xanthus encapsulin system, two cargo proteins called EncB and EncC contribute to iron mineralization. Here, we show that EncB and EncC generate iron‐containing minerals with distinct chemical compositions, suggesting that the composition of stored ...
Harry B. McDowell +2 more
wiley +1 more source
Hniezdne zoskupenia vtákov v ochranných dubových lesoch sa môžu podobať zoskupeniam v prírodných dubinách / Breeding bird assemblages in protection oak forests can be similar to those in natural oak forests [PDF]
Protection oak forests, preserving soils from erosion on steep rocky slopes, ridges and screes, could be important habitats for breeding bird assemblages similar to those in natural oak forests.
Štefan PAVLÍK
doaj
‘Guide and Prejudice’— How Argonautes recognize targets across domains of life
Argonaute proteins use short nucleic‐acid guides to locate and regulate specific targets across all domains of life. Despite striking diversity—from human gene silencing to bacterial immune defence—all Argonautes share a conserved three‐stage recognition logic: guide‐directed sampling, progressive target pairing with a conformational checkpoint and ...
Jack P. K. Bravo
wiley +1 more source

