Results 1 to 10 of about 5,568,459 (279)

Application of a Spacer-nick Gene-targeting Approach to Repair Disease-causing Mutations with Increased Safety

open access: yesBio-Protocol, 2023
The CRISPR/Cas9 system is a powerful tool for gene repair that holds great potential for gene therapy to cure monogenic diseases. Despite intensive improvement, the safety of this system remains a major clinical concern.
Ngoc Tung Tran   +5 more
doaj   +1 more source

Median Distance Model for Likert-Type Items in Contingency Table Analysis

open access: yesRevstat Statistical Journal, 2023
Likert-type items (questions) are a widely used scale in questionnaire design. The “neutral” or “undecided” option may lead to misinterpretation and confusion about the results.
Serpil Aktas Altunay   +1 more
doaj   +1 more source

Water for all [PDF]

open access: yesARQ, 2023
Using passive water collection strategies, La Maraña and ARECMA, together with the community of Mariana, design and construct an autonomous emergency water supply system, crystallizing decades of struggle for the right to water in the face of a fragile ...
Francisco Susmel, La Maraña, ARECMA
doaj   +1 more source

A CRISPR/Cas9-mediated screen identifies determinants of early plasma cell differentiation

open access: yesFrontiers in Immunology, 2023
IntroductionThe differentiation of B cells into antibody-secreting plasma cells depends on cell division-coupled, epigenetic and other cellular processes that are incompletely understood.MethodsWe have developed a CRISPR/Cas9-based screen that models an ...
Ermeng Xiong   +8 more
doaj   +1 more source

Affective Neuroscience: The Suitability of a Web App to Monitor Affective States at Work

open access: yesFrontiers in Psychology, 2021
This work describes in detail the use of a new tool, a web-app, based on the conceptual framework of affective neuroscience, in particular on Panksepp’s 7 basic emotional systems.
Paola Manfredi   +2 more
doaj   +1 more source

Generation of a NES-mScarlet Red Fluorescent Reporter Human iPSC Line for Live Cell Imaging and Flow Cytometric Analysis and Sorting Using CRISPR-Cas9-Mediated Gene Editing

open access: yesCells, 2022
Advances in the regenerative stem cell field have propelled the generation of tissue-specific cells in the culture dish for subsequent transplantation, drug screening purposes, or the elucidation of disease mechanisms.
Parivash Nouri   +5 more
doaj   +1 more source

Production and Bioassay of a Diffusible Factor that Induces Gametophyte-to-Sporophyte Developmental Reprogramming in the Brown Alga Ectocarpus

open access: yesBio-Protocol, 2020
The brown alga Ectocarpus has a haploid-diploid life cycle that involves alternation between two multicellular generations, the sporophyte and the gametophyte.
Haiqin Yao   +8 more
doaj   +1 more source

Association Between Platelet-to-Lymphocyte Ratio and Preterm Necrotizing Enterocolitis

open access: yesFrontiers in Pediatrics, 2021
Introduction: Necrotizing enterocolitis (NEC) is a fatal condition for very-low-birth-weight infants. Necrotizing enterocolitis is a multi-factor phenomenon that results in intestinal mucosal damage and leads to intestinal necrosis.
Juan Zheng   +4 more
doaj   +1 more source

Two-stage two-locus models in genome-wide association. [PDF]

open access: yesPLoS Genetics, 2006
Studies in model organisms suggest that epistasis may play an important role in the etiology of complex diseases and traits in humans. With the era of large-scale genome-wide association studies fast approaching, it is important to quantify whether it ...
David M Evans   +3 more
doaj   +1 more source

Stochastic models (cooperative and non-cooperative) for NMR analysis of the hetero-association of aromatic molecules in aqueous solution [PDF]

open access: yes, 2006
Stochastic cooperative (STOCH-C) and non-cooperative (STOCH-NC) models have been developed for NMR analysis of the hetero-association of aromatic compounds in solution, in order to take into account all physically meaningful association reactions of ...
Davies, David B.   +2 more
core   +1 more source

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