Results 1 to 10 of about 37,178 (323)

Monitoring Protein Complexation with Polyphosphazene Polyelectrolyte Using Automated Dynamic Light Scattering Titration and Asymmetric Flow Field Flow Fractionation and Protein Recognition Immunoassay [PDF]

ACS Polymers Au, 2023
Polyphosphazenes represent a class of intrinsically flexible polyelectrolytes with potent immunoadjuvant activity, which is enabled through non-covalent self-assembly with antigenic proteins by charge complexation.
Michael Lueckheide, Alexander Marin, Harichandra D. Tagad, Nicholas D. Posey, Vivek M. Prabhu, Alexander K. Andrianov   +5 more
doaj   +3 more sources

Revisiting the dynamics of proteins during milk powder hydration using asymmetric flow field-flow fractionation (AF4) [PDF]

Current Research in Food Science, 2021
The dynamics of β-casein and casein micelles in the reconstitution of skim milk were revisited in this study. β-casein migrates into casein micelles upon an increase in temperatures due to an increase in the hydrophobic effect and lower calcium-phosphate
Anouk Lie-Piang, Mats Leeman, Alejandra Castro, Erik Börjesson, Lars Nilsson   +4 more
doaj   +3 more sources

Adeno-associated Virus Virus-like Particle Characterization via Orthogonal Methods: Nanoelectrospray Differential Mobility Analysis, Asymmetric Flow Field-Flow Fractionation, and Atomic Force Microscopy [PDF]

ACS Omega, 2021
Adeno-associated virus (AAV)-based virus-like particles (VLPs) are thriving vectors of choice in the biopharmaceutical field of gene therapy. Here, a method to investigate purified AAV serotype 8 (AAV8) batches via a nanoelectrospray gas-phase mobility ...
Samuele Zoratto, Victor U. Weiss, Gernot Friedbacher, Carsten Buengener, Robert Pletzenauer, Alexandra Foettinger-Vacha, Michael Graninger, Guenter Allmaier   +7 more
doaj   +3 more sources

Asymmetric-flow field-flow fractionation of prions reveals a strain-specific continuum of quaternary structures with protease resistance developing at a hydrodynamic radius of 15 nm. [PDF]

PLoS Pathogens, 2021
Prion diseases are transmissible neurodegenerative disorders that affect mammals, including humans. The central molecular event is the conversion of cellular prion glycoprotein, PrPC, into a plethora of assemblies, PrPSc, associated with disease ...
Leonardo M Cortez, Satish K Nemani, Camilo Duque Velásquez, Aishwarya Sriraman, YongLiang Wang, Holger Wille, Debbie McKenzie, Valerie L Sim   +7 more
doaj   +3 more sources

Advanced Characterization of Lignin Nanoparticles by Asymmetric Flow-Field Flow Fractionation. [PDF]

ChemSusChem
The unique properties of lignin nanoparticles (LNPs) – uniform shape, surface charge and nanoscale – carry great potential for the desired material utilization of technical lignins.
Kohlhuber N, Sulaeva I, Zou T, Musl O, Mildner R, Renneckar S, Österberg M, Rosenau T, Potthast A.   +8 more
europepmc   +5 more sources

Identification of distinct nanoparticles and subsets of extracellular vesicles by asymmetric flow field-flow fractionation. [PDF]

Nat Cell Biol, 2018
The heterogeneity of exosomal populations has hindered our understanding of their biogenesis, molecular composition, biodistribution and functions. By employing asymmetric flow field-flow fractionation (AF4), we identified two exosome subpopulations ...
Zhang H, Freitas D, Kim HS, Fabijanic K, Li Z, Chen H, Mark MT, Molina H, Martin AB, Bojmar L, Fang J, Rampersaud S, Hoshino A, Matei I, Kenific CM, Nakajima M, Mutvei AP, Sansone P, Buehring W, Wang H, Jimenez JP, Cohen-Gould L, Paknejad N, Brendel M, Manova-Todorova K, Magalhães A, Ferreira JA, Osório H, Silva AM, Massey A, Cubillos-Ruiz JR, Galletti G, Giannakakou P, Cuervo AM, Blenis J, Schwartz R, Brady MS, Peinado H, Bromberg J, Matsui H, Reis CA, Lyden D.   +41 more
europepmc   +4 more sources

Core lipid, surface lipid and apolipoprotein composition analysis of lipoprotein particles as a function of particle size in one workflow integrating asymmetric flow field-flow fractionation and liquid chromatography-tandem mass spectrometry. [PDF]

PLoS ONE, 2018
Lipoproteins are complex molecular assemblies that are key participants in the intricate cascade of extracellular lipid metabolism with important consequences in the formation of atherosclerotic lesions and the development of cardiovascular disease ...
Zsuzsanna Kuklenyik, Jeffery I Jones, Michael S Gardner, David M Schieltz, Bryan A Parks, Christopher A Toth, Jon C Rees, Michael L Andrews, Kayla Carter, Antony K Lehtikoski, Lisa G McWilliams, Yulanda M Williamson, Kevin P Bierbaum, James L Pirkle, John R Barr   +14 more
doaj   +3 more sources

Investigating the Impact of Hydrophobic Polymer Segments on the Self-Assembly Behavior of Supramolecular Cyclic Peptide Systems via Asymmetric-Flow Field Flow Fractionation. [PDF]

Macromolecules, 2023
The present study examines the behavior of cyclic peptide polymer conjugates that have been designed to combine their self-assembling ability via H-bonding with the properties of amphiphilic diblock copolymers.
Kariuki M, Rho JY, Hall SCL, Perrier S.
europepmc   +2 more sources

Asymmetric flow field-flow fractionation as a multifunctional technique for the characterization of polymeric nanocarriers. [PDF]

Drug Deliv Transl Res, 2021
The importance of polymeric nanocarriers in the field of drug delivery is ever-increasing, and the accurate characterization of their properties is paramount to understand and predict their behavior.
Quattrini F, Berrecoso G, Crecente-Campo J, Alonso MJ.   +3 more
europepmc   +2 more sources

Characterization and purification of pentameric chimeric protein particles using asymmetric flow field-flow fractionation coupled with multiple detectors. [PDF]

Anal Bioanal Chem, 2021
Porcine circovirus causes the post-weaning multi-systemic wasting syndrome. Despite the existence of commercial vaccines, the development of more effective and cheaper vaccines is expected.
Kotoucek J, Hezova R, Vrablikova A, Hubatka F, Kulich P, Macaulay S, Roessner D, Raska M, Psikal I, Turanek J.   +9 more
europepmc   +2 more sources