Insights into the internal structures of nanogels using a versatile asymmetric-flow field-flow fractionation method. [PDF]
Nanoscale Adv, 2020 Poly(N-isopropylacrylamide) (pNIPAM) nanogels are a highly researched type of colloidal material. In this work, we establish a versatile asymmetric-flow field-flow fractionation (AF4) method that can provide high resolution particle sizing and also ...
Niezabitowska E +7 more
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Asymmetric flow field-flow fractionation coupled to surface plasmon resonance detection for analysis of therapeutic proteins in blood serum. [PDF]
Anal Bioanal Chem, 2021 Coupling of surface plasmon resonance (SPR) detection to asymmetric flow field-flow fractionation (AF4) offers the possibility to study active fractions of bio-separations on real samples, such as serum and saliva, including the assessment of activity of
Leeman M +5 more
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Optimization of hyphenated asymmetric flow field-flow fractionation for the analysis of silver nanoparticles in aqueous solutions. [PDF]
Anal Bioanal Chem, 2021 The extensive use of silver nanoparticles (AgNPs) in consumer products, medicine, and industry leads to their release into the environment. Thus, a characterization of the concentration, size, fate, and toxicity of AgNPs under environmental conditions is
Geißler F +3 more
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Asymmetric flow field flow fractionation methods for virus purification [PDF]
Journal of Chromatography A, 2016 Detailed biochemical and biophysical characterization of viruses requires viral preparations of high quantity and purity. The optimization of virus production and purification is an essential, but laborious and time-consuming process. Asymmetric flow field flow fractionation (AF4) is an attractive alternative method for virus purification because it is
Eskelin, Katri Johanna +5 more
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Measuring Particle Size Distribution by Asymmetric Flow Field Flow Fractionation: A Powerful Method for the Preclinical Characterization of Lipid-Based Nanoparticles. [PDF]
Mol Pharm, 2019 Particle size distribution and stability are key attributes for the evaluation of the safety and efficacy profile of medical nanoparticles (Med-NPs). Measuring particle average size and particle size distribution is a challenging task which requires the ...
Caputo F +7 more
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Asymmetric flow field‐flow fractionation of liposomes: optimization of fractionation variables [PDF]
Journal of Separation Science, 2009 AbstractThe purpose of this study was to investigate the influence of ionic strength of the carrier liquid, cross flow rate, focus flow rate, and sample load on the retention behavior of liposomes in asymmetric flow field‐flow fractionation (AF4). Two differently prepared samples of large unilamellar vesicles (LUV) were used. Experiments were performed
Stefan Hupfeld +2 more
exaly +3 more sources
Determination and Characterization of Gold Nanoparticles in Liquor Using Asymmetric Flow Field-Flow Fractionation Hyphenated with Inductively Coupled Plasma Mass Spectrometry [PDF]
MoleculesThe EU has approved the usage of gold as a food additive (E175) and it has been applied in numerous foods for coloring and decoration purposes. Different from the general assumption that edible gold is mainly present in the form of flakes or external ...
Bin Li +4 more
doaj +2 more sources
Journal of Chromatography AThe present study explores the capability of asymmetrical flow field-flow fractionation (AF4) coupled online with diode array (DAD), fluorescence detectors (FLD), multi-angle light scattering (MALS) and dynamic light scattering (DLS) to characterize ...
Rocco Gasco +3 more
semanticscholar +2 more sources
The Application of Field-Flow Fractionation to the Analysis of Nanomedicines [PDF]
British Journal of Pharmacy, 2022 The combination of field-flow fractionation with powerful leading-edge detectors can be applied to the measurement of nanomaterial physicochemical properties, and the creation of harmonized robust measurement protocols.
Karim Daramy +4 more
doaj +2 more sources
Asymmetric-flow field-flow fractionation technology for exomere and small extracellular vesicle separation and characterization. [PDF]
Nat Protoc, 2019 Zhang H, Lyden D.
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