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Asymmetric Flow Field-Flow Fractionation in the Field of Nanomedicine

Analytical Chemistry, 2014
Asymmetric flow field-flow fractionation (AF4) is a widely used and versatile technique in the family of field-flow fractionations, indicated by a rapidly increasing number of publications. It represents a gentle separation and characterization method, where nonspecific interactions are reduced to a minimum, allows a broad separation range from several
Michael, Wagner   +4 more
openaire   +2 more sources

Membrane-organic solute interactions in asymmetric flow field flow fractionation: Interplay of hydrodynamic and electrostatic forces.

Science of the Total Environment, 2022
The structure and size characterization of organic matter (OM) using flow field-flow fractionation (FFFF) is interesting due to the numerous interactions of OM in aquatic systems and water treatment processes.
Akhil Gopalakrishnan   +2 more
semanticscholar   +1 more source

Field and flow programming in frit-inlet asymmetrical flow field-flow fractionation

Journal of Chromatography A, 2002
The separation of wide molecular mass (Mr) ranges of macromolecules using frit inlet asymmetrical flow field-flow fractionation (FI-AFlFFF) has been improved by implementing a combination of field and flow programming. In this first implementation, field strength (governed by the cross flow-rate through the membrane-covered accumulation wall) is ...
Myeong Hee, Moon   +3 more
openaire   +2 more sources

Selective quantification of nanoplastics in environmental matrices by asymmetric flow field-flow fractionation with total organic carbon detection.

Chemical Communications, 2021
Nanoplastics are of rapidly emerging concern as ubiquitous environmental pollutants. However, fate and transport assessments are currently hindered by a need for new analytical methods that can selectively quantify nanoplastics in environmental matrices.
Marfua Mowla   +2 more
semanticscholar   +1 more source

Release, transfer and partition of fluorescent dyes from polymeric nanocarriers to serum proteins monitored by asymmetric flow field-flow fractionation.

Journal of Chromatography A, 2021
Fluorescent probes are used in drug nanocarrier pre-clinical studies or as active compounds in theranostics and photodynamic therapy. In the biological medium, nanoparticles interact with proteins, which can result in the off-target release of their ...
M. A. de Oliveira   +7 more
semanticscholar   +1 more source

Fractionation of Humic Substances by Asymmetrical Flow Field-Flow Fractionation

Chromatographia, 2005
Asymmetrical flow field-flow fractionation was used to investigate the effects of pH and ionic strength of the buffer, and the binding of polyaromatic hydrocarbons (PAHs) on the particle sizes of humic substances (HSs). Particle sizes were greater when HSs were present in acidic medium with phosphate buffer than when they were present in alkaline ...
G. Yohannes   +3 more
openaire   +1 more source

Analysis of plant ribosomes with asymmetric flow field-flow fractionation

Analytical and Bioanalytical Chemistry, 2013
Ribosome profiling is a technique used to separate ribosomal subunits, 80S ribosomes (monosomes), and polyribosomes (polysomes) from other RNA-protein complexes. It is traditionally performed in sucrose gradients. In this study, we used asymmetric flow field-flow fractionation (AsFlFFF) to characterize ribosome profiles of Nicotiana benthamiana plants.
Eskelin Katri   +2 more
openaire   +2 more sources

The use of asymmetrical flow field-flow fractionation in pharmaceutics and biopharmaceutics

European Journal of Pharmaceutics and Biopharmaceutics, 2004
Field-flow fractionation (FFF) is a family of flexible analytical fractionating techniques which have the advantage that the separation of analytes is achieved, solely through the interaction of the sample with an external, perpendicular physical field, rather than by the interaction with a stationary phase.
Wolfgang, Fraunhofer, Gerhard, Winter
openaire   +2 more sources

Miniaturization of Frit Inlet Asymmetrical Flow Field-Flow Fractionation

Analytical Chemistry, 2004
A miniaturized frit inlet asymmetrical flow field-flow fractionation (mFI-AFlFFF) channel has been constructed and tested for the separation of proteins. By scaling down the geometrical channel dimension of a conventional FI-AFlFFF system, flow rate ranges that can be manipulated were decreased to 20-30 microL/min, which reduces the injection amount of
Dukjin, Kang, Myeong Hee, Moon
openaire   +2 more sources

Characterization of the molecular properties and allergenicity (IgE-binding capacity) of β-lactoglobulin by heat treatment using asymmetric-flow field-flow fractionation and ultra-performance liquid chromatography quadrupole time of flight mass chromatography.

Food Chemistry, 2021
In this study, the heat product (90 °C, 10 min) of β-lactoglobulin (β-LG) was analyzed by asymmetric-flow field-flow fractionation (AF4) to observe the effect of heat treatment.
Yang Li   +5 more
semanticscholar   +1 more source

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