Results 211 to 220 of about 34,866 (268)

Development of Two-Dimensional Offline Coupling of Asymmetrical Flow Field-Flow Fractionation and Capillary Electrophoresis for the Separation of a Five-Component Submicrometer Particle Mixture.

Analytical Chemistry, 2023
Submicrometer colloidal particles are widely applied in a variety of industrial products. While precise size and surface charge control is crucial to the stability and functionality of these materials, a tool to determine these properties with sufficient
Meng Jing, Wei Gao, Paul Hutchins
semanticscholar   +1 more source

Analysis of complex particle mixtures by asymmetrical flow field-flow fractionation coupled to inductively coupled plasma time-of-flight mass spectrometry.

Journal of Chromatography A, 2021
Asymmetrical flow field-flow fractionation (AF4) hyphenated with inductively coupled plasma-mass spectrometry (ICP-MS) has been widely used to characterize metal containing particles.
Olga Meili-Borovinskaya, F. Meier, R. Drexel, Mohammed Baalousha, L. Flamigni, A. Hegetschweiler, T. Kraus   +6 more
semanticscholar   +1 more source

Study on effects of preparation method on the structure and antioxidant activity of protein-Tremella fuciformis polysaccharide complexes by asymmetrical flow field-flow fractionation.

Food Chemistry, 2022
Oxidation is an essential biological process for human life. In this study, low density lipoprotein-Tremella fuciformis polysaccharide (LDL-TFP) complexes were prepared by electrostatic and covalent methods.
Xue Chen, Yi-Ling Tian, J. Zhang, Yue-Qiu Li, Wen-Hui Zhang, J. Zhang, Yuwei Dou, Hai-Yang Dou   +7 more
semanticscholar   +1 more source

Asymmetric Flow Field-Flow Fractionation in the Field of Nanomedicine

Analytical Chemistry, 2014
Asymmetric flow field-flow fractionation (AF4) is a widely used and versatile technique in the family of field-flow fractionations, indicated by a rapidly increasing number of publications. It represents a gentle separation and characterization method, where nonspecific interactions are reduced to a minimum, allows a broad separation range from several
Michael, Wagner, Stephan, Holzschuh, Anja, Traeger, Alfred, Fahr, Ulrich S, Schubert   +4 more
openaire   +2 more sources

Separation and characterization of extracellular vesicles from human plasma by asymmetrical flow field-flow fractionation.

Analytica Chimica Acta, 2020
It is a big challenge to isolate extracellular vesicles (EVs) from human plasma because of the contamination from high abundant lipoproteins, such as high density lipoprotein (HDL) and low density lipoprotein particles (LDL).
Bowen Wu, Xiulan Chen, Ji-Feng Wang, Xiao-Qing Qing, Zhipeng Wang, Xiang Ding, Zhen-Sheng Xie, Lili Niu, Xiao-Jing Guo, Tan-Xi Cai, Xiang-Qian Guo, Fuquan Yang   +11 more
semanticscholar   +1 more source

Analysis of Peptides using Asymmetrical Flow Field-flow Fractionation (AF4)

Journal of Pharmaceutical Sciences, 2021
While asymmetrical flow field-flow fractionation (AF4) has been widely used for separation of high molecular weight species and even particles, its ability to resolve lower molecular weight species has rarely been explored. Over the course of many projects, we have discovered that AF4 can be an effective analytical method for separating peptides from ...
Ryan R, Manning, Ryan E, Holcomb, Derrick S, Katayama, Robert W, Payne, Joshua M, Stillahn, Charles S, Henry, Mark Cornell, Manning   +6 more
openaire   +2 more sources

Analysis of plant ribosomes with asymmetric flow field-flow fractionation

Analytical and Bioanalytical Chemistry, 2013
Ribosome profiling is a technique used to separate ribosomal subunits, 80S ribosomes (monosomes), and polyribosomes (polysomes) from other RNA-protein complexes. It is traditionally performed in sucrose gradients. In this study, we used asymmetric flow field-flow fractionation (AsFlFFF) to characterize ribosome profiles of Nicotiana benthamiana plants.
Eskelin Katri, Pitkanen Leena, Tuomainen Paivi   +2 more
openaire   +2 more sources

Fractionation of Humic Substances by Asymmetrical Flow Field-Flow Fractionation

Chromatographia, 2005
Asymmetrical flow field-flow fractionation was used to investigate the effects of pH and ionic strength of the buffer, and the binding of polyaromatic hydrocarbons (PAHs) on the particle sizes of humic substances (HSs). Particle sizes were greater when HSs were present in acidic medium with phosphate buffer than when they were present in alkaline ...
G. Yohannes, S. K. Wiedmer, M. Jussila, M.-L. Riekkola   +3 more
openaire   +1 more source

Miniaturization of Frit Inlet Asymmetrical Flow Field-Flow Fractionation

Analytical Chemistry, 2004
A miniaturized frit inlet asymmetrical flow field-flow fractionation (mFI-AFlFFF) channel has been constructed and tested for the separation of proteins. By scaling down the geometrical channel dimension of a conventional FI-AFlFFF system, flow rate ranges that can be manipulated were decreased to 20-30 microL/min, which reduces the injection amount of
Dukjin, Kang, Myeong Hee, Moon
openaire   +2 more sources

Fractionation of Prion Protein Aggregates by Asymmetrical Flow Field‐Flow Fractionation

2006
Achieving the successful separation and analysis of amyloid and other large protein aggregates can be a difficult proposition. Field-flow fractionation (FFF) is a flow-based separation method like chromatography; however, FFF is capable of high-resolution separations in the absence of a stationary matrix.
Jay R, Silveira, Andrew G, Hughson, Byron, Caughey   +2 more
openaire   +2 more sources