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The use of asymmetrical flow field-flow fractionation in pharmaceutics and biopharmaceutics
European Journal of Pharmaceutics and Biopharmaceutics, 2004Field-flow fractionation (FFF) is a family of flexible analytical fractionating techniques which have the advantage that the separation of analytes is achieved, solely through the interaction of the sample with an external, perpendicular physical field, rather than by the interaction with a stationary phase.
Wolfgang, Fraunhofer, Gerhard, Winter
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Asymmetrical flow field-flow fractionation for the analysis of PEG-asparaginase
Talanta, 2016Monomethoxypolyethylene glycol L-asparaginase (PEG-ASNASE) is the PEGylated version of the enzyme L-asparaginase (ASNASE). Both are used for remission induction in acute lymphoblastic leukemia (ALL) and non-Hodgkin's lymphoma (NHL). The treatment control is generally carried out by performing activity assays, though methods to determine the actual ...
C, John +4 more
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International Journal of Biological Macromolecules
Natural polysaccharides exhibit a wide range of biological activities, which are closely related to their structural characteristics, including their molecular weight distribution, size, monosaccharide composition, glycosidic bond types and spatial ...
Zehong Chen +5 more
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Natural polysaccharides exhibit a wide range of biological activities, which are closely related to their structural characteristics, including their molecular weight distribution, size, monosaccharide composition, glycosidic bond types and spatial ...
Zehong Chen +5 more
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Food Chemistry
Soybean 7S globulin (7S) is a main allergen in soybean. In this study, 7S was modified with jujube polysaccharide (JP) via the Maillard reaction. The effects of ultrasound-assisted hydrogen peroxide (US/H2O2) treatment on the degradation degree of JP ...
Jiaqi Li +6 more
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Soybean 7S globulin (7S) is a main allergen in soybean. In this study, 7S was modified with jujube polysaccharide (JP) via the Maillard reaction. The effects of ultrasound-assisted hydrogen peroxide (US/H2O2) treatment on the degradation degree of JP ...
Jiaqi Li +6 more
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Analytica Chimica Acta, 2020
Exosomes are extracellular vesicles that mediate intercellular communication, immune response, and tumour metastasis. However, exosome isolation from the blood is complicated because their size and density are similar to those of blood lipoproteins. Here,
Young Beom Kim +3 more
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Exosomes are extracellular vesicles that mediate intercellular communication, immune response, and tumour metastasis. However, exosome isolation from the blood is complicated because their size and density are similar to those of blood lipoproteins. Here,
Young Beom Kim +3 more
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Asymmetric Flow Field-Flow Fractionation Investigation of Magnetopolyplexes
Macromolecular Chemistry and Physics, 2015Asymmetric flow field-flow fractionation (AF4) is an analytical separation technique capable of providing information on different parameters of complex systems in a single measurement. Although it shows promise to be an important tool in the field of gene therapy, AF4 has only occasionally been used to study gene delivery vectors such as polyplexes ...
Haladjova E. +6 more
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Present situation and development trends of asymmetrical flow field-flow fractionation
Chinese Journal of Chromatography, 2017Field-flow fractionation (FFF) is a kind of mature separation technologies in the field of bioanalysis, feasible of separating analytes with the differences of certain physical and chemical properties by the combination effects of two orthogonal force fields (flow field and external force field).
Qihui, Liang +3 more
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Food Chemistry, 2021
Insight into the structure and conformation characteristics of starch that influence its enzyme susceptibility is import for its potential application.
Wen-Hui Zhang +5 more
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Insight into the structure and conformation characteristics of starch that influence its enzyme susceptibility is import for its potential application.
Wen-Hui Zhang +5 more
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Circular Asymmetrical Flow Field-Flow Fractionation for the Semipreparative Separation of Particles
Analytical Chemistry, 2003A new technique for the separation and characterization of particles and polymers based on asymmetrical flow field-flow fractionation was developed. The new circular asymmetrical flow field-flow fractionation instrument (CAFFFE) resembles a quasi-parallel arrangement of 12 individual flow channels.
Michael, Maskos, Wolfgang, Schupp
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Optimization of asymmetrical flow field-flow fractionation
LC GC North America, 2011Asymmetrical flow field-flow fractionation can be used on a routine basis to separate high molecular weight compounds as an alternative to size-exclusion chromatography (SEC), but the principle of separation is very different and a different approach is required for optimization.
Qureshi, R.N., Kok, W.T.
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