Recovery, overloading, and protein interactions in asymmetrical flow field-flow fractionation. [PDF]
Anal Bioanal Chem, 2019 In asymmetrical flow field-flow fractionation (AF4), similar to other separation techniques, mass recovery and overloading require special attention in order to obtain quantitative results.
Marioli M, Kok WT.
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Asymmetrical Flow Field-Flow Fractionation on Virus and Virus-Like Particle Applications. [PDF]
Microorganisms, 2019 Asymmetrical flow field-flow fractionation (AF4) separates sample components based on their sizes in the absence of a stationary phase. It is well suited for high molecular weight samples such as virus-sized particles.
Eskelin K, Poranen MM, Oksanen HM.
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Nanoparticle separation with a miniaturized asymmetrical flow field-flow fractionation cartridge [PDF]
Frontiers in Chemistry, 2015 Asymmetrical Flow Field-Flow Fractionation (AF4) is a separation technique applicable to particles over a wide size range. Despite the many advantages of AF4, its adoption in routine particle analysis is somewhat limited by the large footprint of ...
David eMüller +5 more
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Electrical asymmetrical flow field-flow fractionation: Fundamentals, evolution, applications, and prospects [PDF]
Journal of Chromatography AAmong the plethora of techniques that conforms the Field-Flow Fractionation (FFF) family, electrical field-flow fractionation (ElFFF) was designed to separate different analytes based on their size and electrophoretic mobility.
Guzmán Bernardo, Francisco Javier +5 more
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Offline Coupling of Asymmetrical Flow Field-Flow Fractionation and Capillary Electrophoresis for Separation of Extracellular Vesicles. [PDF]
Anal Chem, 2022 Extracellular vesicles (EVs) play important roles in cell-to-cell communications and carry high potential as markers targeted in disease diagnosis, prognosis, and therapeutic development.
Gao Z, Hutchins Z, Li Z, Zhong W.
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Enhancing Extracellular Vesicle Analysis by Integration of Large-Volume Sample Stacking in Capillary Electrophoresis with Asymmetrical Flow Field-Flow Fractionation. [PDF]
Anal Chem, 2023 Extracellular vesicles (EVs) play important roles in cell-cell communication and pathological development. Cargo profiling for the EVs present in clinical specimens can provide valuable insights into their functions and help discover effective EV-based ...
Gao Z +4 more
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Analysis of Silicon Quantum Dots and Serum Proteins Interactions Using Asymmetrical Flow Field-Flow Fractionation. [PDF]
Langmuir, 2023 Semiconductor nanocrystals or quantum dots (QDs) have gained significant attention in biomedical research as versatile probes for imaging, sensing, and therapies.
Nagarajan U +4 more
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Characterizing Non-covalent Protein Complexes Using Asymmetrical Flow Field-Flow Fractionation On-Line Coupled to Native Mass Spectrometry. [PDF]
Anal Chem, 2023 We report an online analytical platform based on the coupling of asymmetrical flow field-flow fractionation (AF4) and native mass spectrometry (nMS) in parallel with UV-absorbance, multi-angle light scattering (MALS), and differential-refractive-index ...
Ventouri IK +9 more
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Reliable assessment of carbon black nanomaterial of a variety of cell culture media for in vitro toxicity assays by asymmetrical flow field-flow fractionation. [PDF]
Anal Bioanal Chem, 2023 Carbon black nanomaterial (CB-NM), as an industrial product with a large number of applications, poses a high risk of exposure, and its impact on health needs to be assessed.
Boughbina-Portolés A +6 more
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Investigation of native and aggregated therapeutic proteins in human plasma with asymmetrical flow field-flow fractionation and mass spectrometry. [PDF]
Anal Bioanal Chem, 2022 Physiochemical degradation of therapeutic proteins in vivo during plasma circulation after administration can have a detrimental effect on their efficacy and safety profile. During drug product development, in vivo animal studies are necessary to explore
Ramm I +5 more
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