Characterization of Silver Nanoparticles under Environmentally Relevant Conditions Using Asymmetrical Flow Field-Flow Fractionation (AF4). [PDF]
PLoS ONE, 2015 The development of methods to monitor manufactured nanomaterials in the environment is one of the crucial areas for the assessment of their risk. More specifically, particle size analysis is a key element, because many properties of nanomaterial are size
Min-Hee Jang, Seungho Lee, Yu Sik Hwang
doaj +2 more sources
Characterization of binding between model protein GA-Z and human serum albumin using asymmetrical flow field-flow fractionation and small angle X-ray scattering. [PDF]
PLoS ONE, 2020 Protein-based drugs often require targeted drug delivery for optimal therapy. A successful strategy to increase the circulation time of the protein in the blood is to link the therapeutic protein with an albumin-binding domain.
Jaeyeong Choi +7 more
doaj +2 more sources
Asymmetrical flow field-flow fractionation and multi-angle laser light scattering: A new analytical approach for the characterisation of insect protein aggregation/polymerisation after heat treatment of Tenebrio molitor larvae [PDF]
Current Research in Food ScienceUnderstanding the structural modifications of insect proteins during the transformation processes used for extract preparation is essential for optimising their functionalities and obtaining high added-value proteins.
Ariel Anouma +3 more
doaj +2 more sources
Asymmetrical flow field-flow-fractionation in pharmaceutical analytics [PDF]
, 2003 Due to enormous progress in recombinant DNA techniques and methodology, a multitude of biosynthetic, pharmaceutically relevant polypeptides and proteins became available in the past decade and have been employed in numerous pharmaceutical products ...
Fraunhofer, Wolfgang
core +2 more sources
AF4-to-SAXS: expanded characterization of nanoparticles and proteins at the P12 BioSAXS beamline [PDF]
Journal of Synchrotron RadiationBiological small-angle X-ray scattering (SAXS) is a versatile and powerful technique for investigating the structural and biophysical properties of biologically and pharmaceutically relevant macromolecules and nanoparticles. SAXS offers detailed insights
Stefano Da Vela +12 more
doaj +2 more sources
Asymmetrical flow field-flow fractionation in purification of an enveloped bacteriophage φ 6 [PDF]
Journal of Chromatography B, 2018 Basic and applied virus research requires specimens that are purified to high homogeneity. Thus, there is much interest in the efficient production and purification of viruses and their subassemblies.
Bamford, Dennis H. +6 more
core +4 more sources
Fractionation of Nanoparticle Matter in Red Wines Using Asymmetrical Flow Field-Flow Fractionation. [PDF]
J Agric Food Chem, 2020 The particle matter of wine is mainly composed of wine colloids and macromolecules. The present work develops a methodology using asymmetrical flow field-flow fractionation coupled with multi-angle light scattering, differential refractive index detector, and ultraviolet detector (AsFlFFF-MALS-dRI-UV) for the fractionation and determination of the ...
Osorio-Macías DE +8 more
europepmc +4 more sources
Asymmetric flow field‐flow fractionation of liposomes: optimization of fractionation variables [PDF]
Journal of Separation Science, 2009 AbstractThe purpose of this study was to investigate the influence of ionic strength of the carrier liquid, cross flow rate, focus flow rate, and sample load on the retention behavior of liposomes in asymmetric flow field‐flow fractionation (AF4). Two differently prepared samples of large unilamellar vesicles (LUV) were used. Experiments were performed
Stefan, Hupfeld +2 more
openaire +3 more sources
Current Research in Food Science, 2021 The dynamics of β-casein and casein micelles in the reconstitution of skim milk were revisited in this study. β-casein migrates into casein micelles upon an increase in temperatures due to an increase in the hydrophobic effect and lower calcium-phosphate
Anouk Lie-Piang +4 more
doaj +1 more source
Asymmetric flow field flow fractionation methods for virus purification [PDF]
Journal of Chromatography A, 2016 Detailed biochemical and biophysical characterization of viruses requires viral preparations of high quantity and purity. The optimization of virus production and purification is an essential, but laborious and time-consuming process. Asymmetric flow field flow fractionation (AF4) is an attractive alternative method for virus purification because it is
Eskelin, Katri Johanna +5 more
openaire +3 more sources